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Effect of cultured medium of human umbilical cord blood-derived mesenchymal stem cells on melanogenic enzyme activity in mouse B16 melanoma cells

  • Original Article
  • Regenerative Medicine
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

The cultured medium of mesenchymal stem cell is a valuable resource for a variety of stem cell-derived cytokines and growth factors with a potential therapeutic effect on skin diseases. Here, we investigated the effect of the cultured medium of human umbilical cord blood-derived mesenchymal stem cell (CM-hCBSC) on melanogenesis in mouse B16 melanoma cells stimulated by α-melanocyte stimulating hormone (α-MSH). Our results show that CM-hCBSC inhibits melanin synthesis due to the decrease in cellular tyrosinase activity in B16 melanoma cells without cytotoxicity. The activity of melanogenic enzymes such as tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2 were evaluated by western blot and semi-quantitative RT-PCR. The CM-hCBSC down-regulated the protein and mRNA expression levels of tyrosinase and TRP-1 in a dose-dependent manner. In addition, TRP-2 protein and mRNA expression levels were found to be down-regulated to a lesser extent. We also measured the level of cytokines in CM-hCBSC using the Luminex total system. It was found that the top enriched cytokine in the CMhCBSC was interukine-6 (IL-6), known as a potent regulator of melanin synthesis together with IL-1α and tumor necrosis factor α (TNF- α). These results indicate that secretary factors including IL-6 in the CM-hCBSC may inhibit melanin synthesis by down-regulating the expression of tyrosinase and TRP1. This study suggests that CMhCBSC has a therapeutic effect as an anti-melanogenic agent and may be effective against hyperpigmentation disorders.

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Authors and Affiliations

  1. Department of Biomedical Sciences, College of Medicine, The Catholic University of Korea, 222 Banpo-daero,Seocho-gu, Seoul, 137-701, Republic of Korea

    Soo-Han Hwang & Heung Jae Chun

  2. Department of Applied Biochemistry, Konkuk University, Chungju, Korea

    Eun-Suk Son, Young-Jin Park & Chang-Soo Lee

  3. Institute of Cell & Tissue Engineering, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 137-701, Republic of Korea

    Heung Jae Chun

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  1. Soo-Han Hwang
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  2. Eun-Suk Son
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  4. Chang-Soo Lee
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Correspondence to Heung Jae Chun.

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Hwang, SH., Son, ES., Park, YJ. et al. Effect of cultured medium of human umbilical cord blood-derived mesenchymal stem cells on melanogenic enzyme activity in mouse B16 melanoma cells. Tissue Eng Regen Med 11, 414–422 (2014). https://doi.org/10.1007/s13770-014-4042-4

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  • DOI: https://doi.org/10.1007/s13770-014-4042-4

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