Abstract
Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in degradation of the extracellular matrix under physiologic and pathogenic conditions. In a previous study of the roles of MMPs in dental caries, MMP-13 was found to be expressed in both normal and caries pulp, implying its involvement in the pathogenesis of dental caries. Little is known about functions of MMP-13 related to caries progression. Therefore, the objective of this study was to analyze MMP-13 expression in crown and root of normal and caries dentin by western blot analysis. Seven extracted teeth were used. They were categorized into three grades. (Grade 1: Caries limited to enamel, Grade 2: A wide range of caries that invaded dentin, Grade 3: A wide range of caries that invaded pulp). Teeth were divided into crown and root. MMP-13 expression in extracted protein from separated coronal and radicular dentin was analyzed by western blotting. Western blotting revealed that MMP-13 was present in radicular dentin, and was differently expressed as caries progressed. MMP-13 was not expressed in the coronal dentin groups. MMP-13 expression was increased with caries progression in root dentin. This is the first study to determine the presence of MMP-13 in the root of the dentin. We found that it was distinctly increased in the root dentin of widely progressed caries. Future studies with larger sample sizes may be needed to clearly elucidate changes in MMP-13 expression with progression of caries.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
R Visse, H Nagase, Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry, Circ Res, 92, 827 (2003).
TH Vu, Z Werb, Matrix metalloproteinases: effectors of development and normal physiology, Genes Dev, 14, 2123 (2000).
MD Sternlicht, Z Werb, How matrix metalloproteinases regulate cell behavior, Annu Rev Cell Dev Biol, 17, 463 (2001).
H Birkedal-Hansen, Role of matrix metalloproteinases in human periodontal diseases, J Periodontol, 64, 474 (1993).
J Ma, U Kitti, O Teronen, et al., Collagenases in different categories of peri-implant vertical bone loss, J Dent Res, 79, 1870 (2000).
L Tjäderhane, H Larjava, T Sorsa, et al., The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions, J Dent Res, 77, 1622 (1998).
L Tjäderhane, H Palosaari, J Wahlgren, et al., Human odontoblast culture method: the expression of collagen and matrix metalloproteinases (MMPs), Adv Dent Res, 15, 55 (2001).
DH Pashley, FR Tay, C Yiu, et al., Collagen degradation by host-derived enzymes during aging, J Dent Res, 83, 216 (2004).
C Chaussain-Miller, F Fioretti, M Goldberg, et al., The role of matrix metalloproteinases (MMPs) in human caries, J Dent Res, 85, 22 (2006).
AP de Souza, RF Gerlach, SR Line, et al., Inhibition of human gingival gelatinases (MMP-2 and MMP-9) by metal salts, Dent Mater, 16, 103 (2000).
MJ Belmar, C Pabst, B Martínez, et al., Gelatinolytic activity in gingival crevicular fluid from teeth with periapical lesions, Oral Surq Oral Med Oral Pathol Oral Radiol Endod, 105, 801 (2008).
M Sulkala, V Pääkkönen, M Larmas, et al., Matrix metalloproteinase-13 (MMP-13, collagenase-3) is highly expressed in human tooth pulp, Connect Tissue Res, 45, 231 (2004).
PW Caufield, AL Griffen, Dental caries. An infectious and transmissible disease, Pediatr Clin North Am, 47, 1001 (2000).
M Sulkala, J Wahlgren, M Larmas, et al., The effects of MMP inhibitors on human salivary MMP activity and caries progression in rats, J Dent Res, 80, 1545 (2001).
A Linde, Dentin matrix proteins: composition and possible functions in calcification, Anat Rec, 224, 154 (1989).
G Embery, R Hall, R Waddington, et al., Proteoglycans in dentinogenesis, Crit Rev Oral Biol Med, 12, 331 (2001).
KD Moses, WT Butler, C Qin, et al., Immunohistochemical study of small integrin-binding ligand, N-linked glycoproteins in reactionary dentin of rat molars at different ages, Eur J Oral Sci, 114, 216 (2006).
JM Freije, I Diez-Itza, M Balbin, et al., Molecular cloning and expression of collagenase-3, a novel human matrix metalloproteinase produced by breast carcinomas, J Biol Chem, 269, 16766 (1994).
MF Leeman, S Curran, GI Murray, et al., The structure, regulation, and function of human matrix metalloproteinase-13, Crit Rev Biochem Mol Biol, 37, 149 (2002).
L Ravanti, L Häkkinen, H Larjava, et al., Transforming growth factor-beta induces collagenase-3 expression by human gingival fibroblasts via p38 mitogen-activated protein kinase, J Biol Chem, 274, 37292 (1999).
N Johansson, U Saarialho-Kere, K Airola, et al., Collagenase-3 (MMP-13) is expressed by hypertrophic chondrocytes, periosteal cells, and osteoblasts during human fetal bone development, Dev Dyn, 208, 387 (1997).
CE Brinckerhoff, JL Rutter, U Benbow, et al., Interstitial collagenases as markers of tumor progression, Clin Cancer Res, 6, 4823 (2000).
V Knauper, C Lopez-Otin, B Smith, et al., Biochemical characterization of human collagenase-3, J Biol Chem, 271, 1544 (1996).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lee, T.Y., Jin, E.J. & Choi, B. MMP-13 expression in coronal and radicular dentin according to caries progression -a pilot study. Tissue Eng Regen Med 10, 317–321 (2013). https://doi.org/10.1007/s13770-013-1095-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13770-013-1095-8