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Effects of nutritional enrichment on acid production from degenerated (non-solventogenic) Clostridium acetobutylicum strain M5

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Abstract

Clostridium acetobutylicum has been used as a microbial platform for the production of butanol, acetone, and butyrate from biomass. This study examined the effect of nutritional enrichment on the production of acetate and butyrate by C. acetobutylicum in culture, and tested whether this nutritional change could shift metabolic flux in these microbial cells. The degenerated (non-solventogenic) C. acetobutylicum M5 strain, which lacks the pSOL1 plasmid that contains genes responsible for solvent production, was cultured in the rich medium, C. acetobutylicum medium 1 (CAM1). As a control, M5 strain was also cultured in clostridial growth medium (CGM). Batch fermentation of M5 strain in CAM1 achieved a cell density of 23.7 (OD600), which was 2.55 times that obtained when these cells were cultured in CGM. Fermentation in CAM1 yielded volumetric acetate and butyrate productivities of 0.42 g/L/h and 1.06 g/L/h, respectively, which were 2.33 and 1.33 times the values obtained in CGM. Nutritional enrichment also increased the acetate-to-butyrate ratio, which was 0.39 g/g for M5 cells grown in CAM1 and 0.25 g/g for those grown in CGM. These findings demonstrate that the tested nutritional enrichment triggers a metabolic shift in the acid production of a degenerated C. acetobutylicum in culture.

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References

  1. Lee SY, Park JH, Jang SH, Nielsen LK, Kim J, Jung KS (2008) Fermentative butanol production by Clostridia. Biotechnol Bioeng 101:209–228

    Article  CAS  PubMed  Google Scholar 

  2. Jones DT, Woods DR (1986) Acetone-butanol fermentation revisited. Microbiol Rev 50:484

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Park S, Kim K, Han S-I, Kim EJ, Choi Y-E (2017) Organic solvent-free lipid extraction from wet Aurantiochytrium sp. biomass for co-production of biodiesel and value-added products. Appl Biol Chem 60:101–108

    Article  CAS  Google Scholar 

  4. Durre P (2007) Biobutanol: an attractive biofuel. Biotechnol J 2:1525–1534

    Article  CAS  PubMed  Google Scholar 

  5. Nolling J, Breton G, Omelchenko MV, Makarova KS, Zeng Q, Gibson R, Lee HM, Dubois J, Qiu D, Hitti J, Wolf YI, Tatusov RL, Sabathe F, Doucette-Stamm L, Soucaille P, Daly MJ, Bennett GN, Koonin EV, Smith DR (2001) Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. J Bacteriol 183:4823–4838

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Grimmler C, Janssen H, Krausse D, Fischer RJ, Bahl H, Durre P, Liebl W, Ehrenreich A (2011) Genome-wide gene expression analysis of the switch between acidogenesis and solventogenesis in continuous cultures of Clostridium acetobutylicum. J Mol Microb Biotechnol 20:1–15

    Article  CAS  Google Scholar 

  7. Jang YS, Lee JY, Lee J, Park JH, Im JA, Eom MH, Lee J, Lee SH, Song H, Cho JH, Seung do Y, Lee SY (2012) Enhanced butanol production obtained by reinforcing the direct butanol-forming route in Clostridium acetobutylicum. mBio 3:e00314-12

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Green EM, Bennett GN (1996) Inactivation of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824. Appl Biochem Biotechnol 57:213

    Article  PubMed  Google Scholar 

  9. Cooksley CM, Zhang Y, Wang H, Redl S, Winzer K, Minton NP (2012) Targeted mutagenesis of the Clostridium acetobutylicum acetone-butanol-ethanol fermentation pathway. Metab Eng 14:630–641

    Article  CAS  PubMed  Google Scholar 

  10. Cornillot E, Nair RV, Papoutsakis ET, Soucaille P (1997) The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain. J Bacteriol 179:5442–5447

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Tomas CA, Alsaker KV, Bonarius HP, Hendriksen WT, Yang H, Beamish JA, Paredes CJ, Papoutsakis ET (2003) DNA array-based transcriptional analysis of asporogenous, nonsolventogenic Clostridium acetobutylicum strains SKO1 and M5. J Bacteriol 185:4539–4547

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Lee JY, Jang YS, Lee J, Papoutsakis ET, Lee SY (2009) Metabolic engineering of Clostridium acetobutylicum M5 for highly selective butanol production. Biotechnol J 4:1432–1440

    Article  CAS  PubMed  Google Scholar 

  13. Choi SJ, Lee J, Jang YS, Park JH, Lee SY, Kim IH (2012) Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum. J Microbiol 50:1063–1066

    Article  CAS  PubMed  Google Scholar 

  14. Duc HD (2016) Biodegradation of 3-chloroaniline by suspended cells and biofilm of Acinetobacter baumannii GFJ1. Appl Biol Chem 59:703–709

    Article  CAS  Google Scholar 

  15. Kim M, Kim N, Han J (2016) Deglycosylation of flavonoid O-glucosides by human intestinal bacteria Enterococcus sp. MRG-2 and Lactococcus sp. MRG-IF-4. Appl Biol Chem 59:443–449

    Article  CAS  Google Scholar 

  16. Lee J, Lee MH, Cho EJ, Lee S (2016) High-yield methods for purification of α-linolenic acid from Perilla frutescens var. japonica oil. Appl Biol Chem 59:89–94

    Article  CAS  Google Scholar 

  17. An DG, Cha MN, Nadarajan SP, Kim BG, Ahn J-H (2016) Bacterial synthesis of four hydroxycinnamic acids. Appl Biol Chem 59:173–179

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We would like to thank E.T. Papoutsakis and G. Bennett for providing strain M5. This work was supported by grants from the Ministry of Science and ICT (MSIT) through the NRF of Korea (NRF-2016R1D1A3B04933184, NRF-2012M1A2A2026556, and NRF-2012M1A2A2026557).

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Authors and Affiliations

  1. Institute of Agriculture and Life Science (IALS), Department of Agricultural Chemistry and Food Science Technology, Division of Applied Life Science (BK21 Plus Program), Gyeongsang National University, Jinju, 52828, Republic of Korea

    Ji Eun Woo & Yu-Sin Jang

  2. Department of Chemical and Biomolecular Engineering (BK21 Plus Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea

    Sang Yup Lee

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  1. Ji Eun Woo
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  2. Sang Yup Lee
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Correspondence to Sang Yup Lee or Yu-Sin Jang.

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Woo, J.E., Lee, S.Y. & Jang, YS. Effects of nutritional enrichment on acid production from degenerated (non-solventogenic) Clostridium acetobutylicum strain M5. Appl Biol Chem 61, 469–472 (2018). https://doi.org/10.1007/s13765-018-0372-6

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