Applied Biological Chemistry

, Volume 60, Issue 2, pp 205–214 | Cite as

Targeted metabolite profiling to evaluate unintended metabolic changes of genetic modification in resveratrol-enriched rice (Oryza sativa L.)

  • Min Sung Kim
  • So-Hyeon Baek
  • Sang Un Park
  • Kyung-Hoan ImEmail author
  • Jae Kwang KimEmail author


Resveratrol-enriched rice (RR) includes the stilbene synthase gene for resveratrol synthesis and the phosphinothricin-N-acetyltransferase gene for glufosinate tolerance. To investigate unintended effects resulting from RR’s genetically modified chemical composition, 56 polar and nonpolar secondary metabolites were analyzed with gas chromatography–mass spectrometry in RR and conventional non-transgenic rice. Rice was cultivated during two seasons along three representative climatic regions in the Republic of Korea. Principal components analysis was used to visualize chemical composition differences among rice samples. The results showed that chemical composition was more influenced by growing year and location than by whether or not the rice was transgenic. Pearson’s correlations and hierarchical clustering analysis also indicated no difference in the biochemical structures of RR versus non-transgenic rice. In addition, the glufosinate-ammonium treatment did not significantly change RR chemical composition.


Gas chromatography–mass spectrometry Metabolomics Multivariate analysis Resveratrol Safety assessment 



This work was supported by a Grant from the Incheon National University Research Grant in 2014, Republic of Korea.

Supplementary material

13765_2017_265_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 15 kb)


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Copyright information

© The Korean Society for Applied Biological Chemistry 2017

Authors and Affiliations

  1. 1.Division of Life Sciences and Convergence Research Center for Insect VectorsIncheon National UniversityIncheonRepublic of Korea
  2. 2.Department of Well-being ResourcesSunchon National UniversitySuncheonRepublic of Korea
  3. 3.Department of Crop ScienceChungnam National UniversityDaejeonRepublic of Korea

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