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Targeted metabolite profiling to evaluate unintended metabolic changes of genetic modification in resveratrol-enriched rice (Oryza sativa L.)

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Abstract

Resveratrol-enriched rice (RR) includes the stilbene synthase gene for resveratrol synthesis and the phosphinothricin-N-acetyltransferase gene for glufosinate tolerance. To investigate unintended effects resulting from RR’s genetically modified chemical composition, 56 polar and nonpolar secondary metabolites were analyzed with gas chromatography–mass spectrometry in RR and conventional non-transgenic rice. Rice was cultivated during two seasons along three representative climatic regions in the Republic of Korea. Principal components analysis was used to visualize chemical composition differences among rice samples. The results showed that chemical composition was more influenced by growing year and location than by whether or not the rice was transgenic. Pearson’s correlations and hierarchical clustering analysis also indicated no difference in the biochemical structures of RR versus non-transgenic rice. In addition, the glufosinate-ammonium treatment did not significantly change RR chemical composition.

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Acknowledgment

This work was supported by a Grant from the Incheon National University Research Grant in 2014, Republic of Korea.

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Authors and Affiliations

  1. Division of Life Sciences and Convergence Research Center for Insect Vectors, Incheon National University, Incheon, 22012, Republic of Korea

    Min Sung Kim, Kyung-Hoan Im & Jae Kwang Kim

  2. Department of Well-being Resources, Sunchon National University, Suncheon, Jeonnam, 57922, Republic of Korea

    So-Hyeon Baek

  3. Department of Crop Science, Chungnam National University, 99 Daehak-Ro, Yuseong-gu, Daejeon, 34134, Republic of Korea

    Sang Un Park

Authors
  1. Min Sung Kim
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  2. So-Hyeon Baek
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  3. Sang Un Park
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  4. Kyung-Hoan Im
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  5. Jae Kwang Kim
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Correspondence to Kyung-Hoan Im or Jae Kwang Kim.

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Kim, M.S., Baek, SH., Park, S.U. et al. Targeted metabolite profiling to evaluate unintended metabolic changes of genetic modification in resveratrol-enriched rice (Oryza sativa L.). Appl Biol Chem 60, 205–214 (2017). https://doi.org/10.1007/s13765-017-0265-0

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