Abstract
Salt-tolerant aerobic denitrifiers can remove nitrate nitrogen (NO3−-N) efficiently from polluted seawater under aerobic conditions. Three salt-tolerant and aerobic denitrifiers, Zobellella sp. MAD-44, Halomonas alkaliphila HRL-9 and Vibrio spp. AD2 were combined for preparing a compound agent named AHM M3. The influence factors (composition proportion, temperature, inoculation amount, shaking speed, and C/N ratio) of aerobic denitrification and the conversion pathway of NO3−-N were investigated. The results showed that there was no antagonism between AD2, HRL-9 and MAD-44, and their addition ratio with 2:2:3 (v/v/v ratio) had the highest denitrification efficiency among all the treatments. Under high-salinity (30‰) conditions, the optimized grow conditions of 30 °C, inoculation amount of 2% (v/v), shaking speed 120 rpm, and C/N ratio 10 were obtained for AHM M3, which significantly removed more NO3−-N than any single strain (i.e., AD2, HRL-9 or MAD-44) (P < 0.05). The substrate NO3−-N (100 mg/L) was completely removed by AHM M3 in 24 h without nitrite nitrogen accumulation. Under the optimized conditions, 17.07, 63.13, and 19.80% of NO3−-N was converted into intracellular nitrogen, gaseous nitrogen, and other products by AHM M3, respectively. These results revealed that AHM M3 had an excellent ability of NO3−-N reduction in high-salinity and aerobic conditions.
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This research was funded by the National Natural Science Fund of China (32273186), the Science & Technology Program of Liaoning Province (2021JH2/10200012, 2021JH2/10200011), the Opening Foundation of Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University) Ministry of Education, China (202211), the Scientific Research Fund Project of the Liaoning Provincial Department of Education (LJKMZ20221099; LJKMZ20221103), and the Innovation Support Program for High-level Talents of Dalian City (2020RQ111).
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Ma, H., Liu, Y., Mu, X. et al. Efficient denitrification by a salt-tolerant aerobic compound bacteria agent AHM M3: optimizing composition and reduction conditions. Int. J. Environ. Sci. Technol. 20, 9865–9880 (2023). https://doi.org/10.1007/s13762-023-04979-6
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DOI: https://doi.org/10.1007/s13762-023-04979-6