Abstract
The textile industry produces high amounts of effluents containing synthetic dyes that are recalcitrant and potentially hazardous to the environment, being azo dyes one of the most commonly used classes. Biological methods for dye degradation are promising, but knowledge of the toxicity of the resulting products is key for assessing their safety discharge on the environment. The present study investigated the potential toxicity of simulated effluents with dyes commonly used in the textile industry (reactive, direct, and basic groups). These effluents were treated by yeasts Candida parapsilosis (HOMOGS20B), Candida pseudoglaebosa (LIIIS36B), and Yarrowia lipolytica (HOMOGST27AB) after which their toxicity was assessed using three different assays in order to represent different trophic levels. Mutagenicity was also evaluated. C. pseudoglaebosa (LIIIS36B) was the most effective, completely decolorizing four of the five simulated effluents. Degradation products resulting from the yeast treatment were detected in the supernatant of the treated effluents and could be aromatic amines. Although the toxicity of the original simulated effluents was high, treated effluents demonstrated a decrease in toxicity and none exhibit mutagenicity. This work shows that the three selected yeasts could have a high potential for dye removal from textile wastewaters without generating toxic end-products, thus being an environmentally safe biological method.
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References
Abe FR, Machado AL, Soares AMVM et al (2019) Life history and behavior effects of synthetic and natural dyes on Daphnia magna. Chemosphere. 236. https://doi.org/10.1016/j.chemosphere.2019.124390
Ali H (2010) Biodegradation of synthetic dyes—a review. Water Air Soil Pollut 213:251–273. https://doi.org/10.1007/s11270-010-0382-4
Ali SS, Al-Tohamy R, Koutra E et al (2021) Valorizing lignin-like dyes and textile dyeing wastewater by a newly constructed lipid-producing and lignin modifying oleaginous yeast consortium valued for biodiesel and bioremediation. J Hazard Mater 403:123575. https://doi.org/10.1016/j.jhazmat.2020.123575
Al-Tohamy R, Kenawy ER, Sun J, Ali SS (2020) Performance of a newly isolated salt-tolerant yeast strain sterigmatomyces halophilus SSA-1575 for azo dye decolorization and detoxification. Front Microbiol 11:1–19. https://doi.org/10.3389/fmicb.2020.01163
Aracagök YD, Cihangir N (2013) Decolorization of reactive black 5 by Yarrowia lipolytica NBRC 1658. Am J Microbiol Res 1:16–20. https://doi.org/10.12691/ajmr-1-2-1
Ayed L, Mahdhi A, Cheref A, Bakhrouf A (2011) Decolorization and degradation of azo dye Methyl Red by an isolated Sphingomonas paucimobilis: biotoxicity and metabolites characterization. Desalination 274:272–277. https://doi.org/10.1016/j.desal.2011.02.024
Bafana A, Chakrabarti T, Muthal P, Kanade G (2009) Detoxification of benzidine-based azo dye by E. gallinarum: time-course study. Ecotoxicol Environ Saf 72:960–964. https://doi.org/10.1016/j.ecoenv.2007.11.013
Ben Mansour H, Houas I, Montassar F et al (2012) Alteration of in vitro and acute in vivo toxicity of textile dyeing wastewater after chemical and biological remediation. Environ Sci Pollut Res 19:2634–2643. https://doi.org/10.1007/s11356-012-0802-7
Croce R, Cinà F, Lombardo A et al (2017) Aquatic toxicity of several textile dye formulations: acute and chronic assays with Daphnia magna and Raphidocelis subcapitata. Ecotoxicol Environ Saf 144:79–87. https://doi.org/10.1016/j.ecoenv.2017.05.046
Danouche M, EL Arroussi H, EL Ghachtouli N (2021) Mycoremediation of synthetic dyes by yeast cells: a sustainable biodegradation approach. Environ Sustain 4:5–22. https://doi.org/10.1007/s42398-020-00150-w
Dawkar VV, Jadhav UU, Jadhav MU et al (2010) Decolorization and detoxification of sulphonated azo dye Red HE7B by Bacillus sp. VUS. World J Microbiol Biotechnol 26:909–916. https://doi.org/10.1007/s11274-009-0252-7
de Almeida EJR, de Andrade AR, Corso CR (2019) Evaluation of the Acid Blue 161 dye degradation through electrochemical oxidation combined with microbiological systems. Int J Environ Sci Technol 16:8185–8196. https://doi.org/10.1007/s13762-019-02377-5
Dellamatrice PM, Silva-Stenico ME, de Moraes LAB et al (2017) Degradation of textile dyes by cyanobacteria. Braz J Microbiol 48:25–31. https://doi.org/10.1016/j.bjm.2016.09.012
Elisangela F, Andrea Z, Fabio DG et al (2009) Biodegradation of textile azo dyes by a facultative Staphylococcus arlettae strain VN-11 using a sequential microaerophilic/aerobic process. Int Biodeterior Biodegrad 63:280–288. https://doi.org/10.1016/j.ibiod.2008.10.003
Franciscon E, Zille A, Fantinatti-Garboggini F et al (2009) Microaerophilic-aerobic sequential decolourization/biodegradation of textile azo dyes by a facultative Klebsiella sp. strain VN-31. Process Biochem 44:446–452. https://doi.org/10.1016/j.procbio.2008.12.009
Ghazy MME, Habashy MM, Mohammady EY (2011) Effects of pH on survival, growth and reproduction rates of the crustacean, daphnia magna. Aust J Basic Appl Sci 5:1–10
Guo G, Tian F, Zhao Y et al (2019) Aerobic decolorization and detoxification of Acid Scarlet GR by a newly isolated salt-tolerant yeast strain Galactomyces geotrichum GG. Int Biodeterior Biodegrad 145:104818. https://doi.org/10.1016/j.ibiod.2019.104818
Guo G, Li X, Tian F, et al (2020) Azo dye decolorization by a halotolerant consortium under microaerophilic conditions. Chemosphere 244:125510. https://doi.org/10.1016/j.chemosphere.2019.125510
Imran M, Crowley DE, Khalid A et al (2015) Microbial biotechnology for decolorization of textile wastewaters. Rev Environ Sci Biotechnol 14:73–92. https://doi.org/10.1007/s11157-014-9344-4
Jadhav SU, Kalme SD, Govindwar SP (2008) Biodegradation of Methyl red by Galactomyces geotrichum MTCC 1360. Int Biodeterior Biodegrad 62:135–142. https://doi.org/10.1016/j.ibiod.2007.12.010
Jadhav JP, Kalyani DC, Telke AA et al (2010) Evaluation of the efficacy of a bacterial consortium for the removal of color, reduction of heavy metals, and toxicity from textile dye effluent. Bioresour Technol 101:165–173. https://doi.org/10.1016/j.biortech.2009.08.027
Khan R, Bhawana P, Fulekar MH (2013) Microbial decolorization and degradation of synthetic dyes: a review. Rev Environ Sci Biotechnol 12:75–97. https://doi.org/10.1007/s11157-012-9287-6
Kristanti RA, Fikri Ahmad Zubir MM, Hadibarata T (2016) Biotransformation studies of cresol red by Absidia spinosa M15. J Environ Manag 172:107–111. https://doi.org/10.1016/J.JENVMAN.2015.11.017
Kurade MB, Waghmode TR, Kagalkar AN, Govindwar SP (2012) Decolorization of textile industry effluent containing disperse dye Scarlet RR by a newly developed bacterial-yeast consortium BL-GG. Chem Eng J 184:33–41. https://doi.org/10.1016/j.cej.2011.12.058
Kurade MB, Waghmode TR, Jadhav MU et al (2015) Bacterial-yeast consortium as an effective biocatalyst for biodegradation of sulphonated azo dye reactive red 198. RSC Adv 5:23046–23056. https://doi.org/10.1039/c4ra15834b
Kurade MB, Waghmode TR, Patil SM et al (2017) Monitoring the gradual biodegradation of dyes in a simulated textile effluent and development of a novel triple layered fixed bed reactor using a bacterium-yeast consortium. Chem Eng J 307:1026–1036. https://doi.org/10.1016/j.cej.2016.09.028
Lade H, Kadam A, Paul D, Govindwar S (2015) Biodegradation and detoxification of textile azo dyes by bacterial consortium under sequential microaerophilic/aerobic processes. EXCLI J 14:158–174. https://doi.org/10.17179/excli2014-642
Mahmoud MS (2016) Decolorization of certain reactive dye from aqueous solution using Baker’s Yeast (Saccharomyces cerevisiae) strain. Hous Build Natl Res Cent J 12:88–98. https://doi.org/10.1016/j.hbrcj.2014.07.005
Martorell MM, Pajot HF, Rovati JI, Figueroa LIC (2012) Optimization of culture medium composition for manganese peroxidase and tyrosinase production during Reactive Black 5 decolourization by the yeast Trichosporon akiyoshidainum. Yeast 29:137–144. https://doi.org/10.1002/yea
Martorell MM, Pajot HF, Ahmed PM, de Figueroa LIC (2017) Biodecoloration of reactive black 5 by the methylotrophic yeast Candida boidinii MM 4035. J Environ Sci (china) 53:78–87. https://doi.org/10.1016/j.jes.2016.01.033
Mata AMT, Pinheiro HM, Lourenço ND (2015) Effect of sequencing batch cycle strategy on the treatment of a simulated textile wastewater with aerobic granular sludge. Biochem Eng J 104:106–114. https://doi.org/10.1016/j.bej.2015.04.005
Mendes M, Cassoni AC, Alves S et al (2022) Screening for a more sustainable solution for decolorization of dyes and textile effluents using Candida and Yarrowia spp. J Environ Manag 307:114421. https://doi.org/10.1016/j.jenvman.2021.114421
OECD (2004) Test No. 202: Daphnia sp. Acute Immobilisation Test. OECD
Pandey A, Singh P, Iyengar L (2007) Bacterial decolorization and degradation of azo dyes. Int Biodeterior Biodegrad 59:73–84. https://doi.org/10.1016/j.ibiod.2006.08.006
Paz A, Carballo J, Pérez MJ, Domínguez JM (2017) Biological treatment of model dyes and textile wastewaters. Chemosphere 181:168–177. https://doi.org/10.1016/j.chemosphere.2017.04.046
Pinheiro HM, Touraud E, Thomas O (2004) Aromatic amines from azo dye reduction: status review with emphasis on direct UV spectrophotometric detection in textile industry wastewaters. Dye Pigment 61:121–139. https://doi.org/10.1016/j.dyepig.2003.10.009
Priac A, Badot P-M, Crini G (2017) Treated wastewater phytotoxicity assessment using Lactuca sativa: focus on germination and root elongation test parameters. C R Biol 340:188–194. https://doi.org/10.1016/J.CRVI.2017.01.002
Raghukumar C, D’Souza-Ticlo D, Verma AK (2008) Treatment of colored effluents with lignin-degrading enzymes: an emerging role of marine-derived fungi. Crit Rev Microbiol 34:189–206. https://doi.org/10.1080/10408410802526044
Rodrigues de Almeida EJ, Christofoletti Mazzeo DE, Deroldo Sommaggio LR et al (2019) Azo dyes degradation and mutagenicity evaluation with a combination of microbiological and oxidative discoloration treatments. Ecotoxicol Environ Saf 183:109484. https://doi.org/10.1016/j.ecoenv.2019.109484
Saratale RG, Saratale GD, Chang JS, Govindwar SP (2011) Bacterial decolorization and degradation of azo dyes: a review. J Taiwan Inst Chem Eng 42:138–157. https://doi.org/10.1016/j.jtice.2010.06.006
Saravanan P, Kumaran S, Bharathi S et al (2021) Bioremediation of synthetic textile dyes using live yeast Pichia pastoris. Environ Technol Innov 22:101442. https://doi.org/10.1016/j.eti.2021.101442
Singh SN (2015) Microbial degradation of synthetic dyes in wastewaters. Springer, Switzerland
Solís M, Solís A, Pérez HI et al (2012) Microbial decolouration of azo dyes: a review. Process Biochem 47:1723–1748. https://doi.org/10.1016/j.procbio.2012.08.014
Song L, Shao Y, Ning S, Tan L (2017) Performance of a newly isolated salt-tolerant yeast strain Pichia occidentalis G1 for degrading and detoxifying azo dyes. Bioresour Technol 233:21–29. https://doi.org/10.1016/j.biortech.2017.02.065
Sudha M, Saranya A, Selvakumar G, Sivakumar N (2014) Microbial degradation of Azo dyes: a review. Int J Curr Microbiol Appl Sci 3:670–690
Tan L, Xu B, Hao J et al (2019) Biodegradation and detoxification of azo dyes by a newly isolated halotolerant yeast Candida tropicalis SYF-1. Environ Eng Sci 36:999–1010. https://doi.org/10.1089/ees.2018.0485
Vacchi FI, Von der Ohe PC, Alburquerque AF et al (2016) Occurrence and risk assessment of an azo dye—the case of disperse red 1. Chemosphere 156:95–100. https://doi.org/10.1016/j.chemosphere.2016.04.121
Wang Y, Xu B, Ning S et al (2021) Magnetically stimulated azo dye biodegradation by a newly isolated osmo-tolerant Candida tropicalis A1 and transcriptomic responses. Ecotoxicol Environ Saf 209:111791. https://doi.org/10.1016/j.ecoenv.2020.111791
Yang Q, Zhang H, Li X et al (2013) Extracellular enzyme production and phylogenetic distribution of yeasts in wastewater treatment systems. Bioresour Technol 129:264–273. https://doi.org/10.1016/j.biortech.2012.11.101
Zaharia C, Suteu D (2012) Textile organic dyes—characteristics, polluting effects and separation/elimination procedures from industrial effluents—a critical overview. Org Pollut Ten Years After Stock Conv Environ Anal Updat
Acknowledgements
Authors wish to express their acknowledgments to AQUITEX S.A. for providing all commercial dyes, simulated effluents, and dye-related information. Financial support was provided by NORTE-01-0247-FEDER-017819 “EcoTex—Desenvolvimento de soluções mais sustentáveis para coloração têxtil,” co-financed by Fundo Europeu de Desenvolvimento Regional (FEDER) through Programa Operacional Regional do Norte (PONorte). Author Marta Mendes also would like to acknowledge financial support by Fundação para a Ciência e Tecnologia (FCT) PHD Grant SFRH/BD/129334/2017. The authors would also like to thank the scientific collaboration under the FCT project UID/Multi/50016/2019.
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by MM and ACC. The first draft of the manuscript was written by MM, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Mendes, M., Cassoni, A.C., Alves, S. et al. Removing color while lowering toxicity: the case for decolorization of textile dyes and simulated effluents with yeasts. Int. J. Environ. Sci. Technol. 21, 13–24 (2024). https://doi.org/10.1007/s13762-023-04969-8
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DOI: https://doi.org/10.1007/s13762-023-04969-8