Abstract
Investment in green chemistry to convert agro-industrial residues into value-added products could be economically feasible. The present study proved the utility of sugarcane bagasse as an inexpensive and easily available raw material for large-scale production of extracellular laccase from a newfound bacterial strain Lysinibacillus macroides LSO isolated from Alexandria paper and pulp industry effluents. To maximize the efficiency of laccases, a sequential optimization strategy focused on computational experimental designs accompanied by a bench-scale bioreactor batch cultivation strategy was adopted. Among the twelve variables analysed, sugarcane bagasse, NH4Cl, CuSO4·5H2O, and MnSO4·H2O were chosen based on their high positive significant impact on laccase productivity via the 2-level Plackett–Burman design (PBD). Rotatable Central Composite Design (RCCD) was exploited to create a polynomial quadratic model correlating the relationship between the four variables and the productivity of laccase. The highest extracellular laccase productivity and specific growth rate (µ) were achieved at the early period of the incubation time through cultivation Lysinibacillus macroides LSO in a 10-L stirred tank bioreactor under batch operation conditions. Laccase yield 7653.2 U L−1 min−1 and µ = 0.024 h−1, respectively, were obtained after 28 h that increased more than 70.861-, 4.181-, and 1.934-fold compared to basal media, PBD, and RCCD, respectively.
Graphic abstract
Utilization of bio-waste for scaling up the production of extracellular bacterial laccase from Lysinibacillus macroides strain LSO via bioreactor scale
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdel-Fattah R, Hesham S, Gohar Y, El-Baz A (2005) Improved production of Pseudomonas aeruginosa uricase by optimization of process parameters through statistical experimental designs. Process Biochem 40:1707–1714. https://doi.org/10.1016/j.procbio.20-04.06.048
Abdel-Fattah YR, El-Enshasy HA, Soliman NA, El-Gendi H (2009) Bioprocess development for production of alkaline protease by Bacillus pseudofirmus Mn6 through statistical experimental designs. J Microbiol Biotechnol 19(4):378–386
Abdelgalil AS, Morsi A, Reyed M, Soliman A (2018) Application of experimental designs for optimization the production of Alcaligenes faecalis nyso laccase. J Sci Ind Res 77:713–722
Al Fattah Amara A (2011) Experimental design for simultaneous production of PHB, mesophilic proteases and lipases. IIUM Eng J 12:155–184. https://doi.org/10.31436/iium-ej.v1-2i2.137
Arora S, Gill P (2000) Laccase production by some white-rot fungi under different nutritional conditions. Bioresour Technol 73:283–285. https://doi.org/10.1016/S0960-8524(99)00141-8
Boulton A, Large J (1977) Synthesis of certain assimilatory and dissimilatory enzymes during bacterial adaptation to growth on trimethylamine. Microbiology 101:151–156. https://doi.org/10.1099/00221287-101-1-151
Box E, Behnken W (1960) Some new three-level designs for the study of quantitative variables. Technometrics 2:455–475. https://doi.org/10.1080/00401706.1960.10489912
Chauhan S, Goradia B, Saxena A (2017) Bacterial laccase: recent update on production, properties and industrial applications. 3 Biotech 7:323–343. https://doi.org/10.1007/-s13205-017-0955-7
Chauhan S, Goradia B, Jha B (2018) Optimization and up-scaling of ionic liquid tolerant and thermo-alkali stable laccase from a marine Staphylococcus arlettae S1–20 using tea waste. J Taiwan Inst Chem E 86:1–8. https://doi.org/10.1016/j.jtice.2018.02.032
Chen Y, Huang C, Wei M, Meng M, Liu H, Yang H (2013) Properties of the newly isolated extracellular thermo-alkali-stable laccase from thermophilic actinomycetes, Thermobifida fusca and its application in dye intermediates oxidation. AMB Express 3:49–58. https://doi.org/10.1186/2191-0855-3-49
Chhaya R, Modi A (2013) Statistical optimization of laccase producing Streptomyces chartreusis by solid-state fermentation. CIBTech J Microbiol 3:8–17
El-Sersy A, Ebrahim A, Abou-Elela M (2010) Response surface methodology as a tool for optimizing the production of antimicrobial agents from Bacillus licheniformis SN 2. Curr Res Bacteriol 3:1–14. https://doi.org/10.3923/crb.2010.1.14
Ferreira F, Dall’Antonia B, Shiga A, Alvim J, Pessoni B (2018) Sugarcane bagasse as a source of carbon for enzyme production by filamentous fungi. Hoehnea 45:134–142. https://doi.org/10.1590/2236-8906-40/2017
Hussein I, Haider H, Aziz M, Hussein A (2017) Determination the optimum conditions of laccase production by a local isolate of Pseudomonas aeruginosa SR3 using lab-scale fermenter. Int J Sci Nat 8:1–10
Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev-/msw054
Lowry H, Rosebrough J, Farr L, Randall J (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Marahatta B, Punyarit P, Bhudisawasdi V, Paupairoj A, Wongkham S, Petmitr S (2006) Polymorphism of glutathione S-transferase omega gene and risk of cancer. Cancer Lett 236:276–281. https://doi.org/10.1016/j.canlet.2005.05.020
Mongkolthanaruk W, Tongbopit S, Bhoonobtong A (2012) Independent behaviour of bacterial laccases to inducers and metal ions during production and activity. Afri J Biotechnol 11:9391–9398. https://doi.org/10.5897/AJB11.3042
Morales-Álvarez ED et al (2017) Plackett-Burman Design for rGILCC1 laccase activity enhancement in Pichia pastoris: concentrated enzyme kinetic characterization. Enz Res. https://doi.org/10.1155/2017/5947581
Morris L (1948) Quantitative determination of carbohydrates with Dreywood’s anthrone reagent. Science (Washington) 107:254–255. https://doi.org/10.1126/science.107.2775-.254
Nakhla D, Haggar S (2014) A proposal to environmentally balanced sugarcane industry in Egypt. Int J Agric Pol Res 2:321–328. https://doi.org/10.15739/IJAPR.003
Neelkant S, Shankar K, Jayalakshmi K, Sreeramulu K (2019) Optimization of conditions for the production of lignocellulolytic enzymes by Sphingobacterium sp. ksn-11 utilizing agro-wastes under submerged condition. Prep Biochem Biotech 49:927–934. https://doi.org/10.1080/10826068.2019.1643735
Pandey A, Soccol C, Nigam P, Soccol V, Vandenberghe L, Mohan R (2000) Biotechnological potential of agro-industrial residues. II: cassava bagasse. Bioresour Technol 74:81–87. https://doi.org/10.1016/S0960-8524(99)00143-1
Piccinno F, Hischier R, Seeger S, Som C (2016) From laboratory to industrial scale: a scale-up framework for chemical processes in life cycle assessment studies. J Clean Prod 135:1085–1097. https://doi.org/10.1016/j.jclepro.2016.06.164
Plackett L, Burman P (1946) The design of optimum multifactorial experiments. Biometrika 33:305–325
Rajeswari M, Bhuvaneswari V (2016) Production of extracellular laccase from the newly isolated Bacillus sp. PK4. Afr J Biotechnol 15:1813–1826. https://doi.org/10.5897/AJB-2016-.15509
Rajeswari M, Bhuvaneswari V (2017) Optimization of laccase production from Bacillus sp. PK4 through statistical design of experiments. Microbiol Biotechnol Lett 45:330–342. https://doi.org/10.4014/mbl.1709.09003
Reksohadiwinoto BS (2019) Novelties of laccase enzyme from bacteria of sago waste. IOP Conference Series: Earth and Environmental Science 406:012013
Sondhi S, Saini K (2019) Response surface-based optimization of laccase production from Bacillus sp. MSK-01 using fruit juice waste as an effective substrate. Heliyon 5:1–8. https://doi.org/10.1016/j.heliyon.2019.e01718
Sondhi S, Sharma P, George N, Chauhan S, Puri N, Gupta N (2015) An extracellular thermo-alkali-stable laccase from Bacillus tequilensis SN4, with a potential to bio-bleach softwood pulp. 3 Biotech 5:175–185. https://doi.org/10.1007/s13205-014-0207-z
Templeton D, Ehrman T (1995) Determination of acid-insoluble lignin in biomass. In: Laboratory Analytical Procedures No. 003, National Renewable Energy Laboratory, Golden, Co.
Unuofin O, Okoh I, Nwodo U (2019a) Maize Stover as a feedstock for enhanced laccase production by two gammaproteobacteria: a solution to agro-industrial waste stockpiling. Ind Crop Prod 129:611–623. https://doi.org/10.1016/j.indcrop.2018.12.043
Unuofin O, Okoh I, Nwodo U (2019b) Utilization of agro-industrial wastes for the production of laccase by Achromobacter xylosoxidans HWN16 and Bordetella bronchiseptica HSO16. J Environ Manage 231:222–231. https://doi.org/10.1016/j.jenvm-an.2018.10.016
Zhu D, Nian L, Rongxian Z, Fiaz A, Weimin Z, Bin Y, Jian W, Alei G, Murillo G, Jianzhong S (2020) Insight into depolymerization mechanism of bacterial Laccase for lignin. ACS Sustain Chem Eng 8:12920–12933. https://doi.org/10.1021/acssuschemeng-.0c03457
Acknowledgements
The authors gratefully acknowledge the A City of Scientific Research and Technological Applications (SRTA-City), Alexandria, 21934, Egypt, for providing financial support for laboratory measurements and analyses of this paper within the framework of SRTA-City Central Laboratories Services.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that there is no conflict of interest regarding the publication of this paper.
Human and animal rights
This article does not contain any studies with human or animal subjects.
Additional information
Editorial responsibility: Samareh Mirkia.
Rights and permissions
About this article
Cite this article
Abdelgalil, S.A., Soliman, N.A., Abo-Zaid, G.A. et al. Bioprocessing strategies for cost-effective large-scale production of bacterial laccase from Lysinibacillus macroides LSO using bio-waste. Int. J. Environ. Sci. Technol. 19, 1633–1652 (2022). https://doi.org/10.1007/s13762-021-03231-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13762-021-03231-3