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Antioxidants in combination with omega-3 fatty acids protect rat neonatal cardiomyocytes against H2O2-induced oxidative stress

  • Original Research
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Nutrafoods

Abstract

Objective

Oxidative stress is involved in the development of cardiovascular injuries. The aim of the current study was to determine whether pretreatment with a combination of antioxidants and omega-3 fatty acids protects rat neonatal cardiomyocytes against H2O2-induced oxidative stress.

Methods

Rat neonatal cardiomyocytes were cultured for 2 h with either olive oil to serve as a control or a combination of antioxidants and omega-3 fatty acids (astaxanthin, lutein/zeaxanthin, vitamin C, vitamin E, vitamin D and krill oil) before oxidative stress was induced by H2O2 for 24 h. Investigations included analysis of reliable markers for oxidative stress.

Results

Pretreatment of rat neonatal cardiomyocytes with a combination of antioxidants and omega-3 fatty acids resulted in a reduction in induced lipid peroxidation measured by 8-iso-prostaglandin F2alpha (8-iso-PGF2α) and malondialdehyde (MDA) levels. In addition, protein oxidation was reduced after calculating the protein carbonyl level.

Conclusion

These findings suggest that a combination of antioxidants and omega-3 fatty acids may represent potential protection against oxidative stress.

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Authors and Affiliations

  1. NutraQ, Enebakkveien 117 A, 0680, Oslo, Norway

    Katja Svennevig

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  1. Katja Svennevig
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Correspondence to Katja Svennevig.

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Svennevig, K. Antioxidants in combination with omega-3 fatty acids protect rat neonatal cardiomyocytes against H2O2-induced oxidative stress. Nutrafoods 12, 97–102 (2013). https://doi.org/10.1007/s13749-013-0034-6

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  • DOI: https://doi.org/10.1007/s13749-013-0034-6

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