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The antioxidant and anti-apoptotic properties of vitamins A, C and E in heart tissue of rats exposed to zinc oxide nanoparticles

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Abstract

Background

The rapidly increasing applications of zinc oxide nanoparticles (ZnO NPs) in various industries have led to growing concerns about their damaging influence on human health. The present research was designed to determine the protective action of vitamins (Vits) A, C and E on the heart toxicity induced by ZnO NPs.

Methods

Fifty-four male Wistar rats were allocated into 9 groups of 6 and then exposed to ZnO NPs (200 mg/kg), water (Control1), olive oil (Control2), Vit A (1000 IU/kg), Vit C (200 mg/kg), Vit E (100 IU/kg) and three groups were co-treated with ZnO and one of the Vits A, C or E. The oxidative stress situation was evaluated by measuring oxidative stress markers and the tissue antioxidant enzyme activity. Besides, the mRNA expression of Bcl-2 and Bax and caspase 3,7 activity were assessed. A histopathological examination was also performed to determine the rate of cardiac injury.

Results

The results indicated that co-administration of ZnO NPs and the aforementioned Vits significantly reduced the total oxidant status and lipid peroxidation relative to the ZnO group (P < 0.05). Furthermore, the supplementation of vitamins, notably Vit E, decreased the ZnO NPs-induced oxidative damage by enhancing the activity of antioxidant enzymes compared to the ZnO NPs-fed rats (P < 0.05). Data also showed the mitigating effects of Vits against ZnO NPs-mediated apoptosis by suppressing the ratio of Bax/Bcl-2 expression and caspase 3,7 activity.

Conclusion

This study highlights the protective role of Vits A, C and E against ZnO NPs cardiotoxicity, though at different levels of effectiveness.

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Funding

This study has been derived from a MSc thesis at Bandar Abbas University of Medical Sciences, Iran. The study was funded by Vice-chancellor for Research and Technology, Hormozgan University of Medical Sciences (No. 980343).

Author information

Authors and Affiliations

  1. Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

    Alireza Ekhlasian

  2. Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

    Ebrahim Eftekhar

  3. Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

    Sajedeh Daei, Roghayeh Abbasalipourkabir & Nasrin Ziamajidi

  4. Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran

    Alireza Nourian

  5. Molecular Medicine Research Center, Hamadan University of Medical Science, Hamadan, Iran

    Nasrin Ziamajidi

Authors
  1. Alireza Ekhlasian
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  3. Sajedeh Daei
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  4. Roghayeh Abbasalipourkabir
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Correspondence to Nasrin Ziamajidi.

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Ethics approval and consent to participate

The experimental procedure was approved at the Faculty of Medicine, at Hormozgan University of Medical Sciences (HUMS), Bandar Abbas, Iran. The research was conducted according to the guidelines for the care and use of laboratory animals of HUMS IR.HUMS.REC.1398.425.

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Ekhlasian, A., Eftekhar, E., Daei, S. et al. The antioxidant and anti-apoptotic properties of vitamins A, C and E in heart tissue of rats exposed to zinc oxide nanoparticles. Mol Biol Rep 50, 2357–2365 (2023). https://doi.org/10.1007/s11033-022-08103-8

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  • DOI: https://doi.org/10.1007/s11033-022-08103-8

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