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Non-Faradaic aptasensor based on NH2-GO/PPy for the detection of 17β-estradiol

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Abstract

17ß-Estradiol (E2) is an endocrine-disrupting chemical that has become a matter of concern across the globe for more than a decade due to potential health risks. This study reports facile methods to fabricate label-free aptasensor based on the non-Faradaic impedance mode using amine-functionalized graphene oxide polypyrrole (NH2-GO/PPy)-modified gold interdigitated electrode for direct capacitive detection of 17β-estradiol. The as-prepared NH2-GO/PPy nanocomposites have been successfully characterized by field-emission scanning electron microscopy ( FESEM), atomic force microscopy, Fourier transform infrared spectroscopy, and electrochemical impedance spectroscopy. The results show that NH2-GO/PPy/IDE was able to detect E2 binding through aptamer conformational change by simply observing the capacitance change before and after immobilization. In addition, the biosensor showed a dynamic range of detection from 100 nM to 1 pM with a low detection limit of about 0.13 pM. The recoveries result also presented in this paper was validated using HPLC exhibited high sensitivity for bisphenol-A (BPA) detection and was successfully applied to detect E2 in real water samples with good recoveries.

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Acknowledgements

The authors gratefully acknowledge the financial support from Ministry of Higher Education under Fundamental Research Grant Scheme with the grant code FRGS /1/2020 /STG02/UKM/02/1. The authors acknowledge financial support from University Kebangsaan Malaysia through Modal Insan fund phase 1/2021.

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Authors and Affiliations

  1. Institute of Microengineering and Nanoelectronics, Research Complex, Universiti Kebangsaan Malaysia, Bangi, Malaysia

    Anjam Waheed, Mohd H. Mat Zaid & M. F. Mohd Razip Wee

  2. Department of Chemical Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia

    Mohd H. Mat Zaid

  3. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Seri Kembangan, Malaysia

    Suhainie Ismail

  4. Pusat Pengajian Citra Universiti, Universiti Kebangsaan Malaysia, Bangi, Malaysia

    Afifuddin H. Hussain

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  1. Anjam Waheed
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  2. Mohd H. Mat Zaid
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  4. Afifuddin H. Hussain
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  5. M. F. Mohd Razip Wee
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Correspondence to M. F. Mohd Razip Wee.

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Waheed, A., Mat Zaid, M.H., Ismail, S. et al. Non-Faradaic aptasensor based on NH2-GO/PPy for the detection of 17β-estradiol. J IRAN CHEM SOC 21, 1213–1223 (2024). https://doi.org/10.1007/s13738-024-02987-y

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