Abstract
A simple and rapid reverse-phase high-performance liquid chromatographic method was used for the determination of propineb and tricyclazole by pre-column derivatization with ethyl iodide. The factors affecting the derivatization reaction and separation conditions were carefully evaluated and optimized. The optimum derivatization reaction conditions were as follows: reaction time = 15 min, reaction temperature = 40 °C, concentration of ethylenediaminetetraacetic acid solution = 30 mM, pH of ethylenediaminetetraacetic acid solution = 10 and volume of ethyl iodide = 0.5 mL. The method was validated as per the Directorate-General Health and Consumer Protection and International Conference on Harmonization guidelines concerning system suitability, specificity, linearity, precision, accuracy, robustness, limit of detection and limit of quantification. The validated method was successfully applied to the commercially available pesticide formulations, yielding excellent and precise results.
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The data are available upon request from the corresponding author.
Abbreviations
- RP-HPLC:
-
Reverse-phase high-performance liquid chromatography
- PDA:
-
Photodiode array detector
- EDTA:
-
Ethylenediaminetetraacetic acid
- mM:
-
Millimolar
- WP:
-
Wettable powder
- STD. DEV.:
-
Standard deviation
- % RSD:
-
Percentage relative standard deviation
- LOD:
-
Limit of detection
- LOQ:
-
Limit of quantification
- W:
-
Number of weights
- RT:
-
Retention time
- A. I.:
-
Active ingredient
- SANCO:
-
Directorate-General Health and Consumer Protection
- ICH:
-
International Conference on Harmonization
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Acknowledgements
The authors are sincerely thankful and grateful to Indofil Industries Limited, Thane, School of Chemical Sciences, KBCNMU, Jalgaon, and Smt. P. K. Kotecha Mahila Mahavidyalaya, Bhusawal, for providing the research facilities and technical support and for granting the necessary resources to carry out this research work with keen interest, help and enthusiastic environment.
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Patil, V.K., Chandorkar, J.G., Seshadri, D.T. et al. A simple and rapid reverse-phase high-performance liquid chromatographic method for the determination of propineb and tricyclazole pesticides from the wettable powder formulation. J IRAN CHEM SOC 20, 2587–2594 (2023). https://doi.org/10.1007/s13738-023-02857-z
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DOI: https://doi.org/10.1007/s13738-023-02857-z