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Interaction of the synthesized anticancer compound of the methyl-glycine 1,10-phenanthroline platinum nitrate with human serum albumin and human hemoglobin proteins by spectroscopy methods and molecular docking

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Abstract

In the present study, the interaction of the novel synthesized platinum complex named methyl-glycine 1,10-phenanthroline platinum nitrate product with two most crucial blood carrier proteins of hemoglobin (Hb) and human serum albumin (HSA) has been investigated. Notably, structural alternation ability of the above anticancer compound was assessed at various temperatures through multispectroscopic techniques such as far-UV circular dichroism (CD) and fluorescence with the participation of the molecular docking targeting Hb and HSA in the systems of the medicine delivery. Fluctuations in the inherent fluorescence intensities of the proteins in consequence of the Pt(II) complex binding represented statics of the quenching system. It should be mentioned that thermodynamic and binding parameters have been calculated through the analysis of the outcomes of the quenching and outcomes of van’t Hoff equation for the two proteins. Theoretical and experimental findings pointed out that the leading force for the Pt(II) complex interaction was electrostatic for HSA and hydrophobic interactions for Hb. Moreover, fluorescence experiments discovered that one binding site would be available because of the Pt(II) complex binding in the two proteins accompanied by a negative Gibbs free energy value. Additionally, the far-UV CD outputs suggested that the Pt(II) complex would cause some variations in the regular secondary and tertiary structures of the HSA as the remarkable going down in the α helical contents of proteins structures at different temperatures equal to 25 °C and 37 °C; however, any noteworthy modifications have not been seen in the respective structures of Hb. However, the interaction between the newly made-up medicine (Pt(II) complex) and the two most crucial blood carrier proteins of the Hb and HSA made a notable shifting in the structures and conformations of the proteins via many fluctuations in the secondary and tertiary structures of the two proteins. Hence, such researches of designing the novel metal anticancer medicines may gather useful data for exploring the more suitable path in the face-off with severe disorders like various cancers through inventing and developing metal medical compounds that are more advantageous and have fewer consequences.

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Acknowledgements

Notably, the Research Council of Kharazmi University would be greatly acknowledged for their financial support.

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Author notes

  1. Omid Abazari, Zahra Shafaei and Adele Divsalar have contributed equally to this work.

Authors and Affiliations

  1. Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

    Omid Abazari, Zahra Shafaei & Adele Divsalar

  2. Department of Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran

    Omid Abazari & Ali Moradi

  3. Chemistry and Engineer Chemistry Research Center, Tehran, Iran

    Mahboubeh Eslami-Moghadam

  4. State Key Laboratory of Oncogenes and Related Genes, Institute for Personalized Medicine, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China

    Behafarid Ghalandari

  5. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Ali Akbar Saboury

  6. Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran

    Ali Akbar Saboury

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  1. Omid Abazari
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Correspondence to Adele Divsalar.

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Abazari, O., Shafaei, Z., Divsalar, A. et al. Interaction of the synthesized anticancer compound of the methyl-glycine 1,10-phenanthroline platinum nitrate with human serum albumin and human hemoglobin proteins by spectroscopy methods and molecular docking. J IRAN CHEM SOC 17, 1601–1614 (2020). https://doi.org/10.1007/s13738-020-01879-1

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