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In vitro and in silico inhibition of α-amylase, α-glucosidase, and aldose reductase by the leaf and callus extracts of Vernonia anthelmintica (L.) Willd.

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Abstract

In the present study, the leaf and callus extracts of Vernonia anthelmintica prepared in three different solvents (methanol, ethyl acetate, and chloroform) were assessed for inhibition of α-amylase (EC 3.2.1.1), α-glucosidase (EC 3.2.1.20) and aldose reductase (EC 1.1.1.21). Both the leaf and callus extracts showed remarkable inhibition against these enzymes. Alpha-amylase inhibition was the highest in ethyl acetate extract of calli and methanolic extract of leaves. Ethyl acetate extract of both leaf and calli showed significant alpha-glucosidase and aldose reductase inhibition. HR-LCMS analysis of ethyl acetate fraction of leaf extract showed the presence of the flavonol rhamnetin. Also, the HPLC chromatogram revealed the presence of rhamnetin in callus extract. Further, in silico docking studies of rhamnetin was done against the above-mentioned enzymes. The in silico analysis revealed that compound rhamnetin showed good enzyme inhibition.

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Acknowledgements

The research work was carried out at the School of Biosciences (DST-PURSE supported), Mahatma Gandhi University, Kottayam, Kerala, India, with the financial support of Kerala State Biodiversity Board. Instrumentation support for analysis of the sample was provided by the Department of Biotechnology, India/MSUB programme, KSCSTE-SARD programme and Inter-University Centre for instrumentation (DST-PURSE) Mahatma Gandhi University. The Grant Number is 838/A1/2016/KSBB.

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  1. School of Biosciences, Mahatma Gandhi University, Priyadarshini Hills P.O, Kottayam, Kerala, 686560, India

    Maya Rajan, Vinaya Chandran, S. Shahena, Y. Anie & Linu Mathew

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Maya Rajan has no conflict of interest. Vinaya Chandran has no conflict of interest. S. Shahena has no conflict of interest. Y. Anie has no conflict of interest. Linu Mathew has no conflict of interest.

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Rajan, M., Chandran, V., Shahena, S. et al. In vitro and in silico inhibition of α-amylase, α-glucosidase, and aldose reductase by the leaf and callus extracts of Vernonia anthelmintica (L.) Willd.. ADV TRADIT MED (ADTM) 22, 125–139 (2022). https://doi.org/10.1007/s13596-020-00533-8

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