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Evaluation of standardized fractions of Basella alba leaves as gastroprotective activity on ulcerated rats

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Abstract

Basella alba L. (Basellaceae) is an Indian spinach (English) which have extensively used in constipation, urticaria, diuretic, demulcent and antiulcers. This study was aimed to evaluate the standardized fractions with kaempferol from ethyl acetate extract of Basella alba leaves as gastroprotective, in vivo antioxidant and antisecretory activities in ulcerated rats. In this work the standardized fractions with kaempferol from ethyl acetate extract i.e. bioactive fraction of Basella alba leaves (BFBA) at a doses of 50, 100 and 200 mg/kg/b.w was administered orally bis in die for the treatment of acute ulcer induced by ethanol, aspirin, cold restraint stress and pylorus ligation. The in vivo antioxidant enzymes estimation were carried out in cold restraint stress induced ulcer whereas, gastric secretion and mucosal parameters were estimated in pylorus ligation induced ulcer models. BFBA produced significant inhibition of the gastric lesions induced by ethanol (22.82–66.30 %), aspirin (33.16–64.79 %), cold restraint stress (14.55–70.89 %) and pylorus ligation (32.89–69.73 %) respectively as compared to ulcerated control groups. The levels of antioxidant enzymes such as lipid peroxidase and superoxide dismutase were decreases, whereas increases in catalase and glutathione peroxidase values were observed. The results of our studied shown that BFBA possess a significant antiulcer and antisecretory activities probably by reducing the secretion of acids and offering antioxidant protection against oxidative stress-induced gastric damage and by enhancing glycoprotein secretion.

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Acknowledgments

The authors are thankful to the Director, R.I.T.M. (Pharmacy), Dr. A.P.J. Abdul Kalam Technical University, Lucknow and Director, N.B.R.I (CSIR), Lucknow, Uttar Pradesh, India for providing all the necessary laboratory facilities.

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  1. R.I.T.M. (Department of Pharmacy), Dr. A.P.J. Abdul Kalam Technical University, Lucknow, Uttar Pradesh, India

    Sunil Kumar Jaiswal

  2. Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute (CSIR), Lucknow, Uttar Pradesh, India

    Ch. V. Rao

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  1. Sunil Kumar Jaiswal
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Correspondence to Sunil Kumar Jaiswal.

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Ethical Statement

The research was carried out according to the rules governing the use of laboratory animals as acceptable internationally and the experimental protocol was approved by the Animal Ethics Committee, (IAEC), CPCSEA, India(Reg. No. 222/2000/205 CPCSEA

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Figure 3

Histopathology of BFBA and Ranitidine treated group in different models of ulcerated rats (Haematoxylin and eosin stain) (DOC 732 kb)

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Jaiswal, S.K., Rao, C.V. Evaluation of standardized fractions of Basella alba leaves as gastroprotective activity on ulcerated rats. Orient Pharm Exp Med 16, 339–347 (2016). https://doi.org/10.1007/s13596-016-0244-8

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