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Hepatoprotective effect of Zataria Multiflora Boiss against malathion-induced oxidative stress in male rats

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Abstract

This study was aimed to evaluate the protective effects of methanolic extract of Zataria Multiflora Boiss (Z. multiflora) against oxidative stress induced by malathion (MT) in male Wistar rats in comparison to N-acetylcysteine (NAC). Rats were randomly divided into 6 groups (n: 8) and treated daily for 28 days. They received MT (150 mg/kg), Z. multiflora methanolic extract (50, 100, and 200 mg/kg), and NAC (200 mg/kg) alone or in combination. This study included a histopathological examination of liver tissue in parallel with measurement of serum aminotransferases. Activities of antioxidant enzymes, total glutathione (GSH), and lipid peroxidation end products in plasma and tissue were also measured. Subacute exposure of rats to MT resulted in a significant morphological change in tissue, an increase in plasma aminotransferases, and oxidative damage. Both NAC and Z. multiflora at the dose of 200 mg/kg significantly reduced MT-induced oxidative stress by inhibiting lipid peroxidation, increasing the content of glutathion, and restoring the activities of superoxide dismutase and catalase. Biochemical and histological observations also showed a dose dependent hepatoprotective effect of Z. multiflora extract against subacute exposure to MT.

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Acknowledgments

The authors would like to thank Mehdi Mehdipour from Toxicology and Pharmacology Deprtmant for his kind assistance.

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Authors and Affiliations

  1. Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman, Iran

    Ahmad Ahmadipour, Mostafa Pournamdari, Ali Mandegari Bamkan, Azam Hosseini, Fateme Moradi Afrapoli & Somayyeh Karami-Mohajeri

  2. Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran

    Fariba Sharififar

  3. Department of Medicinal Chemistry, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

    Mostafa Pournamdari

  4. Department of Pharmacology &Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

    Ali Mandegari Bamkan & Somayyeh Karami-Mohajeri

Authors
  1. Ahmad Ahmadipour
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  2. Fariba Sharififar
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  3. Mostafa Pournamdari
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  4. Ali Mandegari Bamkan
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  5. Azam Hosseini
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  6. Fateme Moradi Afrapoli
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  7. Somayyeh Karami-Mohajeri
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Corresponding author

Correspondence to Somayyeh Karami-Mohajeri.

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Ethical Statement

The research was carried out according to the rules governing the use of laboratory animals as acceptable internationally and the experimental protocol was approved by the Animal Ethics Committee, the Kerman Neuroscience Research Center (Registration Number: (EC/KNRC/92–30).

Funding

This study was funded by Kerman University of Medical Science (grant number: 94/10/60/51,456).

Conflict of Interest

The authors declare there is no conflict of interest.

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Ahmadipour, A., Sharififar, F., Pournamdari, M. et al. Hepatoprotective effect of Zataria Multiflora Boiss against malathion-induced oxidative stress in male rats. Orient Pharm Exp Med 16, 287–293 (2016). https://doi.org/10.1007/s13596-016-0238-6

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