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Hepatoprotective effects of Meconopsis aculeata extract against CCl4 induced oxidative damage in rats

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Abstract

The objective of the present study was to investigate the hepatoprotective effects of methanol extract of Meconopsis aculeata, an important medicinal plant of Kashmir Himalayas, against CCl4 induced oxidative damage in rats. Acute hepatotoxicity was induced in Wistar albino rats by single intraperitoneal injection of CCl4 at 1 ml/kg body weight dose. Methanol extract of M. aculeata was orally administered at the doses of 100, 200 and 300 mg/kg body weight/day for 14 days. The results revealed that administration of CCl4 caused a significant increase in serum AST, ALT and LDH levels as compared to the control group (p < 0.001). Additionally, there was a significant decrease in the level of hepatic GSH, GPx, GST, SOD and CAT activities associated with a significant increase of MDA content in CCl4 treated group compared to those of the control group. However, the treatment with methanol extract of M. aculeata prevented these alterations and maintained the antioxidant status. The hepatoprotective property of the extract was further confirmed by histopathological analysis, wherein the extract was shown to prevent neutrophil infiltration and tissue necrosis in the liver samples of treated animals. The results of the present investigation indicate that methanol extract of M. aculeata possesses potent hepatoprotective activity, possibly due to its antioxidant properties.

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Acknowledgements

The authors would like to acknowledge Department of Biochemistry (DST-FIST assisted), University of Kashmir for providing all the necessary facilities throughout this study. This study was not supported by any funding agency.

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Authors and Affiliations

  1. Department of Biochemistry, University of Kashmir, Srinagar, 190006, India

    Sumaya Hassan, Muzaffar Ahmad Bhat, Nasreena Sajjad, Rohaya Ali & Rabia Hamid

  2. Clinical Biochemistry, University of Kashmir, Srinagar, 190006, India

    Showkat Ahmad Ganie

  3. Department of Nanotechnology, University of Kashmir, Srinagar, 190006, India

    Rabia Hamid

Authors
  1. Sumaya Hassan
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  2. Muzaffar Ahmad Bhat
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  3. Nasreena Sajjad
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  4. Rohaya Ali
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  5. Showkat Ahmad Ganie
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  6. Rabia Hamid
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Correspondence to Showkat Ahmad Ganie or Rabia Hamid.

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Ethical statement

The procedures performed in this study were carried in accordance with the ethical standards of the Animal Ethics Committee, University of Kashmir, Srinagar and approved under registration No. KU/2015/07, dated 21-08-2015.

Conflict of interest

Sumaya Hassan has no conflict of interest. Muzaffar Ahmad Bhat has no conflict of interest. Nasreena Sajjad has no conflict of interest. Rohaya Ali has no conflict of interest. Showkat Ahmad Ganie has no conflict of interest. Rabia Hamid has no conflict of interest.

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Hassan, S., Bhat, M.A., Sajjad, N. et al. Hepatoprotective effects of Meconopsis aculeata extract against CCl4 induced oxidative damage in rats. ADV TRADIT MED (ADTM) 20, 545–554 (2020). https://doi.org/10.1007/s13596-020-00490-2

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