Abstract
The crude ethanolic extract of the bark of Ceriops decandra (Griff.) Ding Hou (Family: Rhizophoraceae) was evaluated for its possible anti-inflammatory and antioxidant activities in different methods. At the dose of 400 mg/kg body weight, the extract showed a significant anti-inflammatory activity in the carrageenan-induced oedema test in rats showing 67.72% reduction in the paw volume (P < 0.01) comparable to that produced by the standard drug indomethacin (69.29%) at 24 h. The percentage inhibition of the oedema paw volume by the 400 mg/kg body weight of the extract was also statistically significant (* P < 0.05 and ** p < 0.01) compared favorably with the indomethacin treated animals at 1, 2, 4, 6 and 24 h. Three complementary test systems for determining the antioxidant activities of the ethanolic extract of C. decandra, namely DPPH free radical scavenging, reducing power, total phenolic contents determination were used for the analysis. In DPPH free radical scavenging test, IC50 value for the ethanolic crude extract was found fairly significant (12.90 μg/ml) while compared to the IC50 value of the reference standards ascorbic acid and butylated hydroxy anisole (2.9372 and 4.81 μg/ml) respectively. In reducing power test the absorbance for ethanolic extract was found to be 1.0900 at 100 μg/ml concentration while compared to 2.8111 and 1.0314 for standard ascorbic acid and BHA respectively at the same concentration. The amount of total phenolic content was also determined and calculated as quite high in the ethanolic extract (309.86 mg/g of gallic acid equivalent). Therefore, the obtained results tend to suggest the probable anti-inflammatory and antioxidant activities of the ethanolic extract of C. decandra bark and justify its use in folkloric remedies.
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References
Anjaneyulu AS, Rao VL (2003) Ceriopsins F and G, diterpenoids from Ceriops decandra. Phytochemistry 62(8):1207–1211
Anjaneyulu AS, Rao VL, Lobkovsky E, Clardy J (2002) Ceriopsin E: a new epoxy ent-kaurene diterpenoid from Ceriops decandra. J Nat Prod 65(4):592–594
Aruoma OI (1994) Nutrition and health aspects of free radicals and antioxidants. Food Chem Toxicol 62:671–683
Atoui AK, Mansouri A, Boskou G, Kefalas P (2005) Tea and herbal infusions: their antioxidant activity and phenolic profile. Food Chem 89:27–36
Cook NC, Samman S (1996) Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary sources. Nutr Biochem 7:66–76
Dehpour AA, Ebrahimzadeh MA, Nabavi SF, Nabavi SM (2009) Antioxidant activity of methanol extract of Ferula assafoetida and its essential oil composition. Grasas Aceites 60(4):405–412
Duh PD, Tu YY, Yen GC (1999) Antioxidant activity of the aqueous extract of harng Jyur (Chrysanthemum morifolium Ramat). Lebensmittel-Wissenschaft und Technol 32:269–277
Duke JA, Wain KK (1981) Medicinal Plants of the World. Longman Group, UK
Evans WC (1989) Trease and Evan’s Pharmacognosy, 3rd edn. University Press, Cambridge, pp 546–547
Garcialeme J, Nakamura L, Leite MP, Rochae SM (1973) Pharmacological analysis of the acute inflammatory process induced in rat’s paw by local injection of carrgeenan and byheating. Br J Pharmacol 48:88–96
Gupta M, Mazumder UK, Sivahkumar T, Vamis MlM, Karki S, Sambathkumar R, Manikadan I (2003) Antioxidant and anti-inflammatory activities of Acalypha fruticosa. Nig J Prod Med 7:25–29
Gutteridge JMC (1993) Free radicals in disease processes: a compilation of cause and consequence. Free Radic Res Commum 19:141–1583
Halliwell B, Cross CE, Gutteridge JMC (1992) Free radicals, antioxidants and human disease: where are we now? J Lab Clin Med 119:598–620
Hou WC, Lin RD, Cheng KT, Hung YT, Cho CH, Chen CH, Hwang SY, Lee MH (2003) Free radical scavenging activity of Taiwanese native plants. Phytomedicine 10:170–175
Kathiresan K, Ramanathan T (1997) Medicinal Plants of Parangipettai Coast. Annamalai University, Chennai
Kulisic T, Radonic A, Katalinic V, Milos M (2004) Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem 85:633–640
Kumara, NKHMR (2001) Identification of strategies to improve research on medicinal plants used in Sri Lanka. In: WHO Symposium. University of Ruhuna, Sri Lanka.
Lanhers MC, Fleurentin J, Dorfman P, Motrier F, Pelt JM (1991) Analgesic, antipyretic and anti-inflammatory properties of Euphorbia hirta. Planta Medica 57:225–231
Mani Senthil Kumar, Goran KT, Roy B, Zothanpuia SDK, Pal SK, Biswas M, Roy P, Adhikari AD, Karmakar S, Sen T (2008) Anti-inflammatory activity of Acanthus ilicifolius. J Ethnopharmacol 120:7–12
Mannalamkunnath AN, Kandasamy K, Subramanian M (2010) Antidiabetic activity of the mangrove species Ceriops decandra in alloxan-induced diabetic rats. J Diabetes 2(2):97–103
Miliauskas G, Venskutonis PR, Beek TA (2004) Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 85:231–237
Mossai JS, Rafatullah S, Gala AM, Al-Yahya MA (1995) Pharmacological studies of Rhus retinorrhaea. Int J Pharmacognosy 33:242–246
Ozaki Y (1990) Anti-inflammatory effects of Curcuma xanthorrhiza Roxb, and its active principle. Chem Pharmaceut Bull 38:1045–1048
Sawadogo WR, Boly R, Lompo M, Some N (2006) Anti-inflammatory, analgesic and antipyretic activities of Dicliptera verticillata. Int J Pharmacol 2:267–273
Sies H (1991) Oxidative stress: introduction. Academic Press, London, pp 650
Silbernagl S, Lang F (2000) Atlas de Poche de Physiopathologie. Medecine-Sciences, 1st edn. Flammarion, pp 320–321
Tanaka M, Kuie CW, Nagashima Y, Taguchi T (1988) Applications of antioxidative Maillard reaction products from histidine and glucose to sardine products. Nippon Suisan Gakkaishi 54:1409–1414
Uddin SJ, Shilpi JA, Barua J, Rouf R (2005) Antinociceptive activity of Ceriops decandra leaf and pneumatophore. Fitoterapia 76(2):261–263
Vinson JA, Dabbagh YA, Serry MM, Jang J (1995) Plant flavonoids, especially tea flavonoid, are powerful antioxidants using an in vitro oxidation model for heart diseases. J Agric and Food Chem 43:2800–2802
Watt JM, Breyer-Brandwijk MG (1962) The Medicinal and Poisonous Plants of Southern and Eastern Africa, E & S Livingstone Ltd, London
Wolfe K, Wu X, Liu RH (2003) Antioxidant activity of apple peels. J Agr Food Chem 51:609–614
Yen GC, Duh PD (1994) Scavenging effect of methanolic extracts of peanut hulls on free-radical and active oxygen species. J Agric Food Chem 42:629–632
Acknowledgements
We are grateful to Mr. Jasim Uddin Chowdhury, The Director of BCSIR Laboratories Dhaka and Ms. Jarifa Begum, CSO & the Director in-charge of BCSIR Laboratories Chittagong for their support and inspirations during this research work.
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Hossain, H., Moniruzzaman, S., Nimmi, I. et al. Anti-inflammatory and antioxidant activities of the ethanolic extract of Ceriops decandra (Griff.) Ding Hou bark. Orient Pharm Exp Med 11, 215–220 (2011). https://doi.org/10.1007/s13596-011-0037-z
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DOI: https://doi.org/10.1007/s13596-011-0037-z