Globalization has facilitated the spread of emerging pests such as the Varroa destructor mite, resulting in the near global distribution of the pest. In South African and Brazilian honey bees, mite-resistant colonies appeared within a decade; in Europe, mite-resistant colonies are rare, but several of these exhibited high levels of “re-capping” behavior. We studied re-capping in Varroa-naïve (UK/Australia) and Varroa-resistant (South Africa and Brazil) populations and found very low and very high levels, respectively, with the resistant populations targeting mite-infested cells. Furthermore, 54% of artificially infested A. m. capensis worker cells were removed after 10 days and 83% of the remaining infested cells were re-capped. Such targeted re-capping of drone cells did not occur. We propose that cell opening is a fundamental trait in mite-resistant populations and that re-capping is an accurate proxy for this behavior.
During the past 70 years, the ectoparasitic “Varroa” mite (Varroa destructor) has spread worldwide and has become the greatest threat for apiculture, killing large numbers of managed Apis mellifera honey bee colonies (Rosenkranz et al. 48), while decimating feral and wild populations (Wenner et al. 56). Many beekeepers originally advocated breeding from stock that survived, but in the vast majority of cases, their colonies ultimately died, since any preexisting defense adaptations were either not sufficiently developed or were overwhelmed by the massive number of mites initially circulating in the population. As such, countries across the Northern Hemisphere, and those (e.g., Argentina, New Zealand) which had imported Northern Hemisphere honey bees that subsequently became infested by Varroa, were forced to use miticides to control mite numbers and to protect their bee populations.
By contrast, and although the evolution of defense mechanisms can occur rapidly (< 100 years) but is rarely seen occurring simultaneously in allopatric populations (Thompson 52), in South Africa and Brazil, their honey bees quickly became resistant to Varroa (Rosenkranz 46). That is, they did not receive nor require the administering of any mite-control methods to ensure their long-term survival, and no population-wide loss of colonies occurred.
The Western honey bee A. mellifera, which consists of approximately 30 geographical subspecies, originated in Africa and appears to have expanded twice into Eurasia, followed by a more recent anthropogenic expansion into the Americas (including Brazil, see below), Asia, and Australasia (Whitfield et al. 57). African honey bees are resilient to many of the pathogens and parasites that often plague (and need to be controlled) in other parts of the world, as evidenced by the limited pest management practiced in Africa (Pirk et al. 42). The Varroa mite arrived in Africa in 1997 to the Cape Region of South Africa (Allsopp 1). This was initially followed by some colony losses; however, these were short-lived, with mite resistance appearing after 3–5 years in the Cape honey bee (A. m. capensis) and 6–7 years in the Savanna honey bee (A. m. scutellata) (Allsopp 1). This pattern of short-lived colony loss prior to the appearance of mite resistance is frequently mentioned in other mite-resistant populations (e.g., Fries et al. 12; Mordecai et al. 33; Oddie et al. 37).
The Africanized honey bee (AHB) is a hybrid between A. m. scutellata from South Africa and East Africa, and various European races, e.g., A. m. iberiensis and A. m. ligustica. In 1957, 26 swarms of A. m. scutellata spread northwards throughout Brazil from Rio Claro, hybridizing with European races to form the AHB which reached the USA in 1990 (Winston 58). In 1971, during this expansion, the Varroa mite arrived in Brazil (Moretto et al. 34) and spread rapidly throughout both the AHB and European honey bees. The subsequent establishment of AHB throughout the tropical and subtropical regions of South America was due, in part, to AHBs’ natural resistance to Varroa (Rosenkranz 46). In both AHB (Camazine 8; Medina et al. 27; Mondragon et al. 32) and A. m. scutellata (Martin and Kryger 23; Nganso et al. 36), poor mite reproduction limits their population growth, although the mechanism(s) by which this occurs has remained unknown.
Targeted selective bee-breeding programs to combat Varroa have been ongoing for decades in both America (Rinderer et al. 45) and Europe (Büchler et al. 7). Selection for traits such as hygienic behavior (based on the removal of killed sealed brood) is being used by beekeepers to help reduce their mite treatment regime, and the Varroa Sensitive Hygiene (VSH) line (developed from Suppression of Mite Reproduction lines) that targets the removal of living mite infested brood (e.g., Harris 16) is undergoing further selection in Hawaii to make it suitable for use in beekeeper operations. Meanwhile, naturally selected mite-resistant populations are being maintained without any mite control measures across a vast range of environments, i.e., that exists across Africa and South and Central America.
Recently, low rates of mite reproduction similar to those found in African and AHB were reported in four European mite-resistant populations (Oddie et al. 37), which raised the possibility that a similar mechanism had arisen in these geographically distinct populations. Oddie et al. (38) then linked the low mite reproduction in these European populations with a high incidence of “re-capping” behavior, when a cell containing a developing pupa has its silk/wax cap partially removed by the worker bees and then resealed with wax, without the removal of the pupa. Although re-capping (even of mite-infested cells) is not a new phenomenon, e.g., both hygienic and non-hygienic colonies re-capped around 90% of artificially created holes in the cell caps (Spivak and Gilliam 51), its importance may have previously been overlooked.
The aim of this study was to investigate mite-naïve populations from Scotland, Isle of Man and Australia, and well-established mite-resistant populations from Brazil (AHB) and South Africa (A. m. scutellata and A. m. capensis), to identify whether re-capping is a reliable proxy for mite-resistance, and whether it is associated with reduced mite reproduction. We then focused on A. m. capensis, which was found to have the highest targeted re-capping of mite-infested cells.
Varroa-mite naïve colonies
During 2018, Varroa-naïve brood samples were obtained from three colonies, each from a different apiary, from across the Island of Colonsay, Scotland, UK, and three colonies from a single apiary belonging to Western Sydney University, Hawkesbury Campus, NSW, Australia. Four additional Varroa-naïve colonies were sampled in 2019 from the Isle of Man, UK.
Mite-resistant AHB colonies, Brazil
The AHB were located at Cruz das Almas, Bahia State, NE Brazil. Re-capping and mite reproduction were studied in February 2018, using six colonies (minimum of 300 worker cells per colony). Re-capping rates and mite infestation data were collected from an additional ten colonies (150–200 worker cells per colony) which were used in a freeze-kill brood removal test.
Mite-resistant South African colonies
Four A. m. scutellata colonies were studied in July 2018 and again in March 2019, while 20 A. m. capensis colonies were studied in July 2018 (n = 3) and in March 2019 (n = 17) (Tables I and S1). As only one A. m. scutellata and two A. m. capensis colonies contained drone brood, in addition, ten A. m. capensis colonies with drone brood were sampled. All colonies are maintained within 20 km of Stellenbosch, Western Cape, South Africa, with the four A. m. scutellata colonies having been moved 800 km from their natural distribution into the area for research purposes. As no mite-susceptible colonies are present in either Brazil or South Africa, no direct comparisons with treated colonies from the same region are possible, although as African bees are the ancestral population both resistant and susceptible populations all originated from Africa (Whitfield et al. 57).
From each colony, a single frame containing mainly purple eyed pupae (e.g., 180–190 hpc) or older worker or drone brood was removed, and on average, 300 cells per colony (Table S1) were examined for re-capping and mite reproduction. To determine whether a cell was re-capped, fine forceps were used to carefully cut around the edge of the cap, which was then inverted to allow the underside to be inspected under a binocular microscope (× 16). If the silk cocoon spun during the first 30 h of the sealed stage (Martin 19) had been partially removed and replaced by wax, it was classified as re-capped. The diameter of the re-capped area for worker brood was estimated to the nearest mm.
Mite reproduction in worker brood
After the re-capping status of the cell had been determined, the pupa was carefully removed and aged according to standard methods (Dietemann et al. 11). If the cell was infested, then all mites, offspring, and shed skins (exuviate) were removed and the mite family reconstructed using the method and developmental chart of Martin (19). Only the 497 infested cells containing yellow-thorax pupae or older, i.e., over 190 h since the cell had been capped, were used in the reproductive calculations (Table S2). The number of mated adult female offspring were counted, that is the cell must also contain a living adult male (evidenced by the exuviate), accompanied by daughters at the correct developmental stage. Only the number of foundresses per cell was determined in the drone brood. Two methods were used to calculate the average number of mated female offspring produced during one reproductive cycle in each of the three study populations, since not all samples are at the same developmental stage; therefore, any mite mortality from the sampling point to bee emergence will not be accounted for. Therefore, firstly, we counted the total number of mated female offspring and divided it by the number of invading foundresses. Secondly, we counted the number of females that would be mated and were assumed to mature prior to bee emergence, but accounted for a further in-cell mortality in cells younger than grey pads, by multiplying the first daughter by 0.94, the second daughter by 0.38, and third daughter by 0.13. These mortality values were based on a study of over 1000 mite families (Martin 19). The results of both calculations are provided (Table S2) and are similar.
Artificial infestation of A. m. capensis cells with Varroa
Since the pattern of Varroa infestation within and among frames is non-random (Fuchs 13), we studied the bees’ ability to detect and remove mite-infested pupae by artificially infesting cells with mites (Boecking 5; Rosenkranz et al. 47) rather than comparing relative changes in infestation levels over a period of time (Harris 15). Therefore, a total of 390 mother mites harvested from A. m. capensis drone brood cells containing a stretched larva were inserted into A. m. capensis worker cells that were less than 1 day post-capping, as indicated by the lack of a completed cocoon (Martin 19). Of these mites, 325 were alive and 65 had died since been collected the previous day. Dead mites were also used for artificial infestation experiments, as comparing rates of detection using living vs dead mites may help indicate cues used to detect infested cells, e.g., movement. A frame containing cells capped within the past 24 h were as evidence by the larvae spinning their cocoons, were removed from each of 11 A. m. capensis colonies over a period of several days. Freshly sealed cells were opened, and a mother mite inserted using a fine paint brush before re-sealing the cell and recording its position on an acetate sheet. After returning the frames to their colonies, an inspection after 24 h revealed any cells removed by the bees as a result of the manipulation. Five days later (6 days post-capping), the number of pupae removed from the artificially infested cells were recorded. After 10 days (11 days post-capping), each frame was removed and any remaining artificially infested cells were inspected for mites, and the state of the cell cap (i.e., re-capped or not) was recorded (Table S3). We also inspected the cap condition of a similar number of neighboring non-infested control cells. Insufficient mites were available to conduct artificial infestation experiments in AHB.
Test for hygienic behavior using freeze-killed brood.
In ten AHB and 11 A. m. capensis colonies, their ability to remove freeze-killed pupae (classic hygienic behavior) was studied to compare with their re-capping rates. An area of purple-eyed pupae or older (> 7 days post-capping) was freeze-killed using liquid nitrogen, and then the number of removed pupae determined after 24 h and 48 h (Table S3). The 48 h data was not analyzed since several of the colonies in both populations had removed 100% of the killed brood by 48 h.
All datasets were non-normally distributed, thus statistical tests included chi-squared, Kolmogorov-Simirnov, Mann-Whitley U, Wilcoxon signed-rank, and Spearman’s rank correlation. The test statistic indicates which test was used if not mentioned in the text.
Cell re-capping and mite reproductive behavior in worker cells
Based on 497 infested cells, we confirmed that the average number of mated female offspring produced per mother foundress during a single reproductive cycle (Wr) was between 0.8 and 0.9 in the three mite-resistant populations (Table I, Table S2), with only 54–55% of the invading mothers reproducing, and producing between 1 and 3 mated female offspring each. Across the ten Varroa-naïve colonies from three different populations, only 0.5% (median) of the worker sealed brood cells were re-capped (Table I, Table S1, Figure S1). In contrast, the median re-capping rates in the Varroa-resistant AHB, A. m. scutellata and A. m. capensis were 35%, 20% and 27%, respectively, although the average infestation rate was < 10% in every mite-resistant population. The re-capping rate of infested cells was always significantly higher than for non-infested cells (AHB U = 17, z = 2.46, p = 0.014; A. m. scutellata U = 0, z = 3.31, p < 0.001; and A. m. capensis U = 10.5, z = 4.41, p < 0.0001) (Table S1, Figure S1). Combining the data from the three populations, we found that the estimated number of viable offspring in re-capped (0.82) and undisturbed (0.76) cells were similar (Table S2). The frequency distribution of the size of the re-capped area (diameter of the opening which had been resealed) of non-infested cells in all four populations followed the same negative trend (Figure 1a–d). In contrast, infested cells all had significantly different size distributions (AHB, D = 0.2051, p = 0.002; A. m. scutellata D = 0.3406, p < 0.0001 and A. m. capensis, D = 1, p = 0.037) due to the re-capped area being larger, i.e., larger than 3 mm, if the cell was infested in all three populations (Figure 1e, f). Furthermore, all re-capped cells in the Varroa-naïve populations were small ranging from 1 to 3 mm. Re-capped cells appeared from the early stages of pupal development and their proportion increased steadily as the pupae developed (Figure 1h). We noticed that some infested cells had been re-capped more than once, as they contained two or three distinct holes cut out of the silk cap.
Cell re-capping and mite infestation in A.m. capensis drone cells
The average infestation level of drone brood was 31 ± 27% (Table I; S1), with cells typically containing multiple foundresses. A total of 330 infested cells (single and multiple infested cells) were invaded by 569 foundress mites. This varied considerably by site; only 5 ± 9% of drone cells were re-capped in nine colonies in the Stellenbosch area (Table S1-R* colonies), whereas three colonies from the Pniel (Table S1-PA* colonies) region in the next valley re-capped 65% of all drone cells. However, the proportion of infested (66%) and non-infested (65%) cells re-capped were similar. Therefore, a pattern of targeting mite-infested drone cells was not seen (Table I; Figure S1). The single drone frame from A. m. scutellata showed the same pattern, of high infestation (57%), low re-capping (2%) and multiple foundresses in cells (Tables I and S1).
Detection and removal of artificially mite infested cells in A. m. capensis
Of the 392 A. m. capensis worker pupae artificially infested with mites (326 alive and 66 dead), only 3% were removed within 24 h, most likely due to the experimental opening and resealing of the cell (manipulation). After 10 days, we found 21 (5%) cells containing no mites or evidence of mites, i.e., mite fecal droppings on the cell wall, which must have escaped during the uncapping/re-capping process, and a further 30 (8%) mites had become sealed into the cell wall and died during the spinning of the pupal cocoon. The mites lost due to manipulation, the re-capping process or being sealed into the cell wall were removed prior to the analysis of removal behavior. Across the 11 colonies, 32% of the infested cells had been removed after six days, and this increased to 54% after 10 days (Table S3). The percentage of dead (47%) and alive (46%) mites removed after 10 days were not significantly different (U = 49.5, p = 0.75). Of the remaining 152 artificially infested cells, 83% had been re-capped, while only 27% of mite free cells ‘control’ cells were recapped (Table S3). Again, typically larger re-capped areas were found in infested cells relative to neighboring non-infested control cells (Figure 2); however, there were no significant differences in sizes of the re-capped area both between non-infested controls and cells containing dead mites trapped in the walls, and between infested cells that contained living or dead mites (Figure 2).
Test for hygienic behavior using freeze-killed brood
Among ten AHB colonies tested for both classic hygienic and re-capping behavior, a Spearman’s rank correlation found no significant correlation ( rs = 0.03, p = 0.93) between the two behaviors after 24 h (Table S3), with 19–98% of the dead brood having been removed in 24 h while the re-capping rates ranged from 4-50%. A similar result (rs = 0.356, p = 0.282) was also found across the 11 A. m. capensis colonies (Table S3), with 48–100% of freeze-killed brood removed while the range of re-capping rates was 12–66%. However, there was a weakly significant positive correlation (rs = 0.67, p = 0.024) between the removal rate of freeze-killed brood and the proportion of artificially infested cells removed (Table S3).
Although we observed the re-capping of brood cells in all colonies, the re-capping rates were lowest in Varroa-naïve, and the highest were consistently found in worker brood of mite-resistant populations from Brazil (AHB) and South Africa (A. m. scutellata and A. m. capensis), as well as Europe (Oddie et al. 38). The key behavior in all these mite-resistant populations appears to be the bees’ ability to detect mite-infested cells, as indicated by consistently higher re-capping rates of infested cells relative to non-infested cells, particularly since infestation rates are typically below 10% (Figure 3; Figure S1). The initial detection of a possible infested cell leads to the opening of a small hole in the cell cap that could allow better access to any volatile or non-volatile cues, i.e., on the pupae, within the sealed cell (see below). If a non-infested cell is opened in error, the hole is re-capped and the disturbed area remains small (1–2 mm), but if infested, the hole is enlarged to 3–4 mm to gain better access (Figure 1). A second trigger, or lack thereof, causes the infested cell to either be re-capped or the pupa to be cannibalized (Figure 4). This idea is in line with previous studies (Gramacho 14; Arathi et al. 3) that found the initial step of detecting diseased brood does not necessarily lead to brood removal, with repeated uncapping and recapping prior to brood removal. The removal of pupae artificially infested with mites was 54% in A. m. capensis (this study), 33% in A. m. scutellata (Cheruiyot et al. 9), 10–25% in AHB (Aumeier et al. 4), and up to 40% in a single mite-resistant population in the Netherlands (Panziera et al. 40). All values are well below the 99% removal of artificially infested worker cells in the mite’s original host, A. cerana (Rath and Drescher 44).
Two recent studies have assumed that genetically derived host factors within the brood prevent the initiation of mite oogenesis, which accounts for the increase in non-reproduction of mites in resistant colonies. For example, Broeckx et al. (6) suggested brood pheromones fall to a level that prevents the mites reproducing, whereas Conlon et al. (10) suggested an ecdysone gene was linked to mite-resistance, since low ecdysone levels may prevent mite oogenesis, hence increasing non-reproduction in mites. However, the greater proportion of non-reproducing mites found in mite-resistant colonies (Martin et al. 25; Broeckx et al. 6) can simply be explained by the behavioral trait of increased disruption of mite reproduction. That is the removal of infested pupae reduces the number of successful mite reproductive cycles, increasing the proportion of non-reproductive mites in subsequent reproductive attempts. The latter may in part account for the lower reproductive values (Wr) found in mite-resistant colonies, since a consistent 50% removal rate will result in 12.5–25% of mites never reproducing due to having been disturbed by the removal of pupae before mating. Since the number of mite reproductive cycles is estimated between 2 and 3 (Martin and Kemp 22), these mites may still invade cells and attempt to reproduce but produce either no offspring or only males (Martin et al. 25), both categories common in African (Martin and Kryger 23), AHB (Medina et al. 27) and this study. This would also explain why reproducing and non-reproducing mites could not be distinguished using DNA micro-satellites (Broeckx et al. 6), since they do not represent two distinct genotypes, just that non-reproducing mites have either run out of sperm or eggs.
Mite reproductivity in re-capped and undisturbed infested cells was similar in this study (Table S2), which was also found in previous studies (Harris et al. 17; Oddie et al. 38). Mondet et al. (30) and Oddie et al. (38) have suggested that the bees are more likely to ignore cells containing non-reproducing mites (selection bias), while preferentially removing cells containing reproducing mites or with more offspring. However, we found no evidence for this in A. m. capensis since the removal rate of cells containing live (reproducing) and dead (non-reproducing) mites were similar, and Panziera et al. (40) also found no relationship between mite reproductive success and brood removal.
In both AHB (Mondragon et al. 32) and African honey bees (A. m. capensis and A. m. scutellata) (Tables I and S1), drone brood frequently becomes heavily infested, which may impact on the honey bee colony and population reproductive success. This can lead to density dependent control of the mite population via the drone brood (Martin and Medina 24), which occurs in A. cerana, either via a reduction in mite offspring survivorship in multiple infested brood (Martin 20) or increased failure for the bee to emerge (Rath 43). Recent studies have found that mite feeding causes A. cerana worker brood to die (Page et al. 39) via injection of a toxic mite salivary protein into the pupae during feeding (Zhang and Han 59), which could explain the high removal rates previously seen (Rath 43). However, this salivary protein has no effect on A. mellifera pupa (Zhang and Han 59).
As only around 27% (Figure 3) of non-infested worker cells are re-capped, this suggests that initial detection of the mite is made without disturbing the cap. While the ability to detect mite infested cells is high in almost all mite resistant colonies (Figure S1 and Oddie et al. 38), it is the trigger to remove the infested pupa that remains error prone since only around 50% of infested cells that are opened are subsequently removed in A. mellifera, and a high proportion of those cells are re-capped. The triggers for the initial detection and subsequent decision to cannibalize the pupa are both currently unknown (Figure 4), although this study indicates that olfaction could be a key factor for the initial detection as several previous studies have proposed (e.g., Rosenkranz et al. 47; Mondet et al. 29; Scannapieco et al. 49), since dead mites elicited a similar re-capping behavior as live mites, as also reported in A. cerana (Rath and Drescher 44). This suggests that motion or associated changes in the pupa (odour or temperature) may not be important as previously suggested by Aumeier and Rosenkranz (3) and Wagoner et al. (54). Nor would the level of oleic acid, which is known to trigger hygienic behavior (McAfee et al. 26), be important, unless also produced by the living mites. Furthermore, as mites sealed into the cell wall by the pupal cocoon did not elicit any increased re-capping response relative to non-infested cells (Figure 2), a volatile odour is a likely candidate. For example, Nazzi et al. (35) found pentadecane (C15H30) was present only in the air of infested cells and the application of Z-(6)-pentadecene increased hygienic behavior, whereas Z-(7)-pentadecene, Z-(8)-heptadecene and pentadecane had no effect. Longer cuticular hydrocarbons are unlikely to be the odour cue due to their lack of volatility, and the pupa’s profile is mimicked precisely by the mite (Kather et al. 18). Rath and Drescher (44) also found that dead mites washed in ethanol were still removed at a high rate in A. cerana. However, Wagoner et al. (55) suggested that two long cuticular hydrocarbons (heptacosene [C27H54] and tritriacontane [C33H66]) removed from the surface of the pupa were associated with the uncapping of infested worker brood. In addition, Mondet et al. (30) suggested changes in the brood pheromone that consists of ten ethyl and methyl esters can be detected between infested and non-infested brood, although this was found using discriminate analysis that is error prone if the sample to variable ratio is not high (Martin and Drijfhout 21; Mitteroecker and Bookstein 28 [Figure 5]).
Why re-capping behavior exists even in the Varroa-naïve populations is unknown, but when non-infested brood are not removed, any cost to colony fitness is minimal. We observed in African honey bee brood invaded by the lesser wax moth (Achroia grisella), cells were frequently re-capped rather than the pupae being removed. Likewise, 57% of the uncapped cells in a colony heavily infested with the greater wax moth (Galleria mellonella) were re-capped within 24 h of uncapping (Villegas and Villa 53). Interestingly, the three A. m. capensis colonies at Pniel were unique in re-capping high numbers of drone cells. These were all survivor colonies from an American Foulbrood (Paenibacillus larvae) outbreak. Therefore, making a small hole in the cell cap may be a general response to allow more detailed investigation of the developing pupa (which may account for the low-level presence of this trait in Varroa-naïve populations). After the arrival of the mites, this behavior appears to have been co-opted and selected for as part of a defense mechanism against Varroa; hence, the re-capping rate is elevated in all infested colonies (Figures 3 and S1), reaching the highest levels in mite-resistant colonies. Throughout Brazil and Africa, beekeeping pest management is minimal and so selective pressures for such traits have always been high. The constant management of a wide range of brood pest and pathogens throughout the Northern Hemisphere removes much of this selective pressure. In this and in previous studies (Oddie et al. 38), the ability to detect mites (Figure S1) and remove infested brood (Table S3) is highly variable. No doubt colony composition plays a role since recapping occurred most in mixed colonies rather than in highly hygienic or highly non-hygienic colonies (Arathi et al. 2).
Mondragon et al. (31) suggested hygienic behavior towards freeze-killed brood may not correlate closely with hygienic behavior towards Varroa mites. We found no correlation between re-capping levels and removal of freeze-killed brood. We did, however, find a weak positive correlation between the ability of a colony to remove freeze-killed brood and the removal of artificial mite-infested cells, which is similar to data from Spivak (50), where colonies selected for their ability to remove freeze-killed brood removed significantly more artificially mite-infested cells than “non-hygienic” colonies in 1 year but not another. Perez and Johnson (41) indicate that task specialization, e.g., hygienic behavior can be used to predict specialization in other related tasks, which may help explain the weak link, between response to freeze-killed brood and removal of living mite infested brood.
It appears that resistance towards Varroa mites in both A. cerana and A. mellifera is following a similar path, that of targeting mites invading worker cells and not drone cells, which will eventually lead to the combined effect of lower mite reproductive success in worker brood and density-dependent control in drone brood. As the ability of the bees to detect mites within worker cells, evidenced by increased re-capping, has arisen naturally in A. mellifera in five different countries, this may prove to be an excellent proxy for mite resistance. The challenge will be selecting for these traits (the abilities to initially detect and subsequently remove infested pupae) while moving away from a regime using insecticides, especially in large commercial beekeeping operations.
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We would like to thank Michael Duncan the technical officer of Western Sydney University for help with providing the honey bee frames. Catherine Taylor and Lauran Agnew of Salford University for assistance with the Varroa-naïve frames. In Brazil, thanks go to Jossimara Neiva de Jesus and Carlos Alfredo Lopes de Carvalho of UFRB for help with and access to the bees, and Christiaan Fransman for field assistance in South Africa. Finally, the British Beekeeping Association for supporting Natasha Reece who worked on the African bees and Hort Frontiers Pollination Fund for supporting L. E. Brettell.
Conflict of interest
The authors declare that they have no conflict of interest.
Manuscript editor: Peter Rosenkranz
Reproduction de Varroa destructor et réoperculation dans les populations d' Apis mellifera résistantes aux acariens.
réoperculation / hygiène / tolérance / résistance / Varroa.
Varroa destructor Reproduktion und Brutzellen-"Recapping" bei varroaresistenten Apis mellifera Populationen.
Recapping / Hygiene/ Toleranz / Resistenz / Varroa
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Martin, S.J., Hawkins, G.P., Brettell, L.E. et al. Varroa destructor reproduction and cell re-capping in mite-resistant Apis mellifera populations. Apidologie 51, 369–381 (2020). https://doi.org/10.1007/s13592-019-00721-9