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Induction of defense responses related to scavenging reactive oxygen species in Ampelopsis species inoculated with Rhizobium vitis

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Abstract

Reactive oxygen species (ROS) is an essential factor that mediates various defense mechanisms against foreign stresses in plants. This study evaluated the content of salicylic acid (SA) and hydrogen peroxide (H2O2) and expression of genes related to ROS scavenging and investigated the content of resveratrol and the expression of genes associated with the pathogenesis-related protein and stilbene synthesis in the leaves of Ampelopsis strains inoculated with Rhizobium vitis. An accumulation of resveratrol and the expression of PR genes were induced in YG10042, YG10045, YG10064, YG11030, YG10075, and YG-Songni4 resistant to the pathogens. A correlation was observed between the ROS content and the gene expression patterns of catalase (CAT) in grapevines after pathogen inoculation. The H2O2 contents, expression of CAT and superoxide dismutase (SOD) gene, and SOD-like activity decreased in Ab2 B-8–2 after pathogen inoculation. The H2O2 content increased with increasing SA content and decreasing expression of CAT gene in YG10042 and YG10045. This suggests that H2O2-dependent pathways in the induction of the defense responses in YG10042 and YG10045 inoculated with R. vitis. In contrast, in YG10064, YG11030, YG10075, and YG-Songni4, in which the defense responses are induced via the H2O2 independent pathway, the expression of CAT and SOD genes increased, whereas the H2O2 content was decreased by the high expression of the CAT gene. Elucidating the roles of ROS accumulation and the expression of related genes would provide useful information in further studies on the mechanism of resistance to disease in Ampelopsis species.

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Acknowledgements

This work was supported by the grant No. PJ016284 from the Agricultural R&D, Rural Development Administration, Republic of Korea.

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  1. Department of Horticulture and Life Science, Yeungnam University, Gyeongsan, 38541, Republic of Korea

    Hae In Lee, Zar Le Myint, Soon Young Ahn & Hae Keun Yun

  2. Department of Fruits, Korea National University of Agriculture and Fisheries, Jeonju, 54874, Republic of Korea

    Seung Heui Kim

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HL performed the overall experiment and data analysis and wrote the manuscript. ZM, SA, SH performed the experiment to confirm the results and wrote the manuscript together. HY designed and managed whole experiments and finalized the manuscript.

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Lee, H.I., Le Myint, Z., Ahn, S.Y. et al. Induction of defense responses related to scavenging reactive oxygen species in Ampelopsis species inoculated with Rhizobium vitis. Hortic. Environ. Biotechnol. 64, 655–668 (2023). https://doi.org/10.1007/s13580-022-00509-4

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