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Chlorogenic acid accumulation and related gene expression in peach fruit

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Abstract

To reveal the molecular mechanism in the accumulation of chlorogenic acids (CGAs) in peach (Prunus persica) fruit during growth and development, CGA contents in the flesh of the three peach cultivars ‘Ruiguang 18’, ‘Heiyoutao’, and ‘Beijingyixianhong’ were determined. The expression levels of CGA metabolism-related genes were analyzed based on transcriptome data (RNA-seq). These candidate genes were then screened and real-time fluorescent quantitative PCR (qRT-PCR) was performed to verify their expression. The results showed that the content of total CGAs, 5-O-caffeoylquinic acid and 3-O-caffeoylquinic acid, in the flesh of ‘Ruiguang 18’ exhibited a decreasing trend during fruit development, and there was a great drop at maturity stage (P < 0.05). The three contents in ‘Heiyoutao’ increased first at stage S2 (P < 0.05) and then decreased significantly (P < 0.05). In ‘Beijingyixianhong’, they stayed stable in the early stages, then total CGAs and 3-O-caffeoylquinic acid decreased significantly at the maturity stage (P < 0.05). RNA-seq transcriptome data analysis and qRT-PCR expression analysis showed that the accumulation of CGAs in fruit flesh was mainly affected by the expression of Prupe.3G100800 (PpHCT) and Prupe.3G107300 (Pp4CL), and their expression levels were highly consistent with total CGA content. Thus, we concluded that Prupe.3G100800 (PpHCT) and Prupe.3G107300 (Pp4CL) are the key genes for CGAs synthesis in peach flesh.

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Acknowledgements

This work was supported by funds of Natural Science Foundation of Jiangsu Province (BK20200278); China Agriculture Research System (CARS-30); Species Conservation Project of Ministry of Agriculture and Rural Affair (19190156); National Crop Germplasm Resources Infrastructure in China (NHGRC2020-NH16).

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Author notes

  1. Juan Yan and Ziwen Su have contributed equally to this work.

Authors and Affiliations

  1. Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, 210014, China

    Juan Yan, Ziwen Su, Shaolei Guo, Binbin Zhang, Zhixiang Cai, Zhijun Shen, Ruijuan Ma & Mingliang Yu

  2. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Ziwen Su

  3. Forest and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China

    Minghao Zhang

Authors
  1. Juan Yan
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  2. Ziwen Su
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  3. Shaolei Guo
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  4. Minghao Zhang
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  5. Binbin Zhang
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  8. Ruijuan Ma
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  9. Mingliang Yu
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Contributions

JY provided the experimental ideas and designed the research; SG, MZ, BZ, and ZC performed the experiments and the analyzed data. JY and ZS drafted the manuscript. ZS, RM, and MY revised the paper. All authors have read and approved the final manuscript.

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Correspondence to Mingliang Yu.

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Communicated by Heakeun Yun.

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Yan, J., Su, Z., Guo, S. et al. Chlorogenic acid accumulation and related gene expression in peach fruit. Hortic. Environ. Biotechnol. 63, 403–411 (2022). https://doi.org/10.1007/s13580-021-00399-y

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