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Aldose reductase inhibition of Rosa hybrida petals and its active component, kaempferol

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Abstract

The aldose reductase inhibitory activity of extracts from Rosa hybrida petals was evaluated, and a bioactive flavonoid, kaempferol, was isolated from petals via bioassay-guided isolation. Kaempferol was isolated from the ethyl acetate fraction of R. hybrida and was shown to exhibit strong inhibition against aldose reductase (IC50 = 0.02 µM). Quantitative analyses of kaempferol in R. hybrida petals, different flower parts, and different Rosa species were also performed using high-performance liquid chromatography. This study is the first to establish that R. hybrida contains kaempferol and that the light-pink petals of this edible rose species exhibit the highest content of the naturally occurring aldose reductase inhibitor. The results of this study suggested the potential role of R. hybrida petals in the treatment and management of diabetic complications via aldose reductase inhibition.

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Acknowledgements

This research was supported by the Chung-Ang University Research Scholarship Grants in 2017, Republic of Korea and by the National Research Foundation of Korea (NRF) funded by the Korea government (MSIP, No. 2017R1A2B4009384).

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Authors and Affiliations

  1. Department of Plant Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea

    Norman G. Quilantang, Carlo A. Limbo, Ju Sung Lee & Sanghyun Lee

  2. Institute of Biology, University of the Philippines, Diliman, 1101, Quezon, Philippines

    Sonia D. Jacinto

  3. Department of Food and Nutrition, Chung-Ang University, Anseong, 17546, Republic of Korea

    Sung-Kwon Moon

Authors
  1. Norman G. Quilantang
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  2. Carlo A. Limbo
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  3. Ju Sung Lee
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  4. Sonia D. Jacinto
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  5. Sung-Kwon Moon
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  6. Sanghyun Lee
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Contributions

NGQ: Kaempferol isolation and identification with AR activity check, CAL: sample extraction and fractionation, JSL: HPLC analysis, SDJ: AR reading and English writing, SKM: sample preparation and HPLC reading, SL: experiment design and manuscript writing.

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Correspondence to Sanghyun Lee.

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Communicated by Jeong Hee Choi.

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Quilantang, N.G., Limbo, C.A., Lee, J.S. et al. Aldose reductase inhibition of Rosa hybrida petals and its active component, kaempferol. Hortic. Environ. Biotechnol. 61, 601–607 (2020). https://doi.org/10.1007/s13580-020-00232-y

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  • DOI: https://doi.org/10.1007/s13580-020-00232-y

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