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The effects of two preservatives on peroxidase activity and total protein content in Strelitzia reginae during cold storage

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An Erratum to this article was published on 01 June 2016

Abstract

This study aimed to assess changes in peroxidase activity and total protein content of cut flowers of Strelitzia reginae that were treated with the chemical preservatives 8-hydroxyquinoline citrate (8-HQC) or chlorine during cold storage. The bases of the cut flowers were immersed in 1.5 L of tap water with 48 h pulsing treatments (a control with no chemical preservatives (0 mg·L-1, 100 mg·L-1 chlorine, 250 mg·L-1 8-HQC or 500 mg·L-1 8-HQC) during cold storage at 10.5°C and 90% relative humidity. The solutions were then replaced with tap water and the cut flowers remained in cold storage for 12 days. For biochemical characterization of peroxidase activity and total protein content, tissue samples (sepals, petals, stems, and bracts) were collected and analyzed at 0, 4, 8, and 12 days after treatment. There was an increase in peroxidase activity in petals, stems, and bracts and a decrease in total protein content, regardless of the treatment used. In this experiment, therefore, differences in peroxidase activity and total protein content due to treatments with 8-HQC or chlorine were not identified.

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Literature Cited

  • Abidallatif SA, Jasm SA (2009) Effect of preservative solutions during wet storage in vase life of zinnia flowers. Iraqi J Agric Sci 40:9–71

    Google Scholar 

  • Ahmad SS, Tahir I, Shahril W (2013) Effect of Different Storage Treatments on Physiology and Postharvest Performance in Cut Scapes of Three Iris Species. J Agric Sci Technol 15:323–331

    CAS  Google Scholar 

  • Azeez A, Sane AP, Bhatnagar D, Nath P (2007) Enhanced expression of serine proteases during floral senescence in Gladiolus. Phytochemistry 68:1352–1357

    Article  CAS  PubMed  Google Scholar 

  • Baker JE, Lieberman M, Anderson JD (1978) Inhibition of ethylene production in fruit slices by rhizobitoxine analogs and free radical scavengers. Plant Physiol 61:886–888

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Battelli R, Lombardi L, Rogers HJ, Picciarelli P, Lorenzi R, Ceccarelli N (2011) Changes in ultrastructure, protease and caspase-like activities during flower senescence in Lilium longiflorum. Plant Sci 180:716–725

    Article  CAS  PubMed  Google Scholar 

  • Barrachina AC, Valero GD, Martínez Serrano RD, Burló CF, Martínez SF, Riquelme BF (2000) Polyamines: biosynthesis, metabolism, and their role in ripening and postharvest handling of fruits. Food Sci Technol Int 6:85–95

    Article  CAS  Google Scholar 

  • Bartoli GC, Guaiamet JJ, Montaldi ER (1997) Ethylene Production and Responses to Exogenous Ethylene in Senescing Petals of Chrysanthemum morifolium RAM cv. Unsei. Plant Sci 124:15–21

    Article  CAS  Google Scholar 

  • Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation stress: A review. Ann Bot 91: 179–194

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bouchereau A, Aziz A, Larher F, Martin TJ (1999) Polyamines and environmental challenges: recent development. Plant Sci 140:103–125

    Article  CAS  Google Scholar 

  • Capell T, Bassie L, Christou P (2004) Modulation of the polyamines biosynthetic pathway in transgenic rice confers tolerance to drought stress. Proc Natl Acad Sci 101:9909–9914

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Çelikel FG, Joyce DC, Faragher JD (2011) Inhibitors of oxidative enzymes affect water uptake and vase life of cut Acacia holosericea and Chamelaucium uncinatum stems. Postharvest Biol Technol 60:149–157

    Article  Google Scholar 

  • Cervelli M, Tavladoraki PDI, Adostino S, Angelini R, Deferico R, Mariottini P (2000) Isolation and caracterization of three polyamine oxidase genes from Zeamays. Plant Physiol Biochem 38:667–677

    Article  CAS  Google Scholar 

  • Chattopadhayay MK, Tiwari BS, Chattopadhayay G, Bose A, Sengupta DN, Ghosh B (2002) Protective role of exogenous polyamines on salinity stressed rice (Oryza sativa) plants. Physiol Plant 116: 192–199

    Article  CAS  PubMed  Google Scholar 

  • Cona A, Rea G, Angelini R, Federico R, Tavladoraki P (2006) Functions of amine oxidases in plant development and defence. Trends Plant Sci 11:80–88

    Article  CAS  PubMed  Google Scholar 

  • Costa LM, Civello MP, Chaves RA (2002) Effect of ethephon and 6-benzylaminopurine on chlorophyll degrading enzymes and a peroxidase-linked chlorophyll bleaching during post-harvest senescence of broccoli (Brassica oleracea L.) at 20°C. Ciência Hoje 183:40–47

    Google Scholar 

  • Costa SA (2009) Postharvest conservation, and physiological symptoms of senescence responses and chilling injury in flower stalks of Heliconia bihai (L). 2009. 83 f. (Doctoral program in Biology) - Universidade Federal Rural de Pernambuco, Recife

    Google Scholar 

  • De Dios P, Matilla AJ, Gallardo M (2006) Flower fertilization and fruit development prompt changes in free polyamines and ethylene in damson plum (Prunus insititia L.). J Plant Physiol 163:86–97

    Article  CAS  PubMed  Google Scholar 

  • Dias-Tagliacozzo GM, Finger FL, Barbosa JG (2005) Postharvest physiology of cut flowers. Rev Bras Hortic Ornamental 11:89–99

    Google Scholar 

  • Eason, JR, Vré L (1995) Ethylene-insensitive floral senescence in Sandersonia aurantiaca (Hook.). N Z J Crop Hortic Sci 23:447–454

    Article  Google Scholar 

  • Eason JR, Ryan JD, Pinkney TT, O’donoghue, EM (2002) Programmed cell death during flowers senescence: isolation and characterization of cysteine proteinases from Sandersonia aurantiaca. Funct Plant Biol 29:1055–1064

    Article  CAS  Google Scholar 

  • El-Hilari F, Ail-Oubahou A, Remah A, Akhayat O (2003) Chilling injury and peroxidase activity change in “Fortune” mandarin fruit during low temperature storage. Bulg J Plant Physiol 29:44–54

    Google Scholar 

  • Heredia JB, Cisneros-Zevallos L (2009) The effects of exogenous ethylene and methyl jasmonate on the accumulation of phenolic antioxidants in selected whole and wounded fresh produce. Food Chem 115:1500–1508

    Article  CAS  Google Scholar 

  • Hossain Z, Mandal AKA, Datta SK, Biswas AK (2006) Decline in ascorbato peroxidase activity-a prerequisite factor for tepal senescence in gladiolus. J Plant Physiol 163:186–194

    Article  CAS  PubMed  Google Scholar 

  • Ferguson, JM, Tekrony DM, Egli DB (1990) Changes during early soybean seed and axes deterioration: II. Lipids. Crop Sci 30:179–182

    Article  CAS  Google Scholar 

  • Flores HE, Galston AW (1982) Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol 69:701–706

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Galston AW, Kaur-Sawhney R (1987) Polyamines and senescence in plants. In W Thompson, E Nothangel, R Huffacker, eds, Plant senescence: Its biochemistry and physiology. Rockville, American Society Plant Physiologist, pp 176–181

    Google Scholar 

  • Garcia-Jimenez P, Just PM, Delgado AM, Robaina RR (2007) Transglutaminase activity decrease during acclimation to hyposaline conditions in marine seaweed Grateloupia doryphora (Rhodophyta, Halymeniaceae). J Plant Physiol 164:367–370

    Article  CAS  PubMed  Google Scholar 

  • Gerailoo S, Ghasemnezhad M (2011) Effect of salicylic acid on antioxidant enzyme activity and petal senescence in ‘yellow island’ cut rose flowers. J Fruit Ornamental Plant Res 19:183–193

    CAS  Google Scholar 

  • Gietl C, Schmid M (2001) Ricinosmes: an organelle for development regulated cell death in senescence in plant tissues. Naturwis 88: 49–58

    Article  CAS  Google Scholar 

  • Guerrero C, Callee M, Reid MS, Valpueta V (1998) Analysis of the expression of two thioproteinase genes from daylily (Hemerocallis spp) during flower senescence. Plant Mol Biol 36:565–571

    Article  CAS  PubMed  Google Scholar 

  • Hörtnsteiner S (2006) Chlorophyll degradation during senescence. Annu Rev Plant Biol 57:55–77

    Article  Google Scholar 

  • Hossain Z, Mandal AKA, Datta SK, Biswas AK (2006) Decline in ascorbate peroxidase activity-a prerequisite factor for tepal senescence in gladiolus. J Plant Physiol 163:186–194

    Article  CAS  PubMed  Google Scholar 

  • Jeevitha S, Sreeramu BS, Raj AVV (2013) Evaluation of the best stage of harvest and pulsing solution for enhancing the vase life of Bird-of-Paradise. Asian J Hortic 8:150–153

    Google Scholar 

  • Jin J, Shan N, Ma N, Bai J, Gao J (2007) Regulation of ascorbate peroxidase at the transcript level is involved in tolerance to postharvest water deficit stress in the cut rose (Rosa hybrida L.) cv. Samantha. Postharvest Biol Technol 40:236–243

    Article  Google Scholar 

  • Jones RB, Hill M (1993) The effect of germicides on the longevity of cut flowers. J Am Soc Hortic Sci 118:350–354

    CAS  Google Scholar 

  • Jones ML, Chaffin GS, Eason JR, Clark DG (2005) Ethylene sensitivity regulates proteolytic activity and cysteine protease gene expression in petunia corollas. J Exp Bot 56:2733–2744

    Article  CAS  PubMed  Google Scholar 

  • Khan MAS, Younis A, Riaz A (2015) Postharvest Treatments for Extending the Vase Life of Cut Stock (Matthiola incana L.) cv. ‘Gold Cut Series’. Agric Commun 3:37–42

    Google Scholar 

  • Kramer GF Wang Y (1989) Correlation of reduced chilling injury with increased spermine and spermidine levels in zucchini squash. J Plant Physiol 76:479–484

    Article  CAS  Google Scholar 

  • Kumar PN, Singh B, Voleti SR (1999) Effect of chemicals on the vase life of standard carnations. J Ornamental Hortic 2:139–140

    Google Scholar 

  • Kuzano T, Berberich T, Tateda C, Takahashi Y (2008) Polyamine: essential factors for growth and survival. Planta 228:367–381

    Article  Google Scholar 

  • Kuznetsov VV Shevyakova NI (2007) Polyamines and stress tolerance of plants. Plant Stress 1:50–71

    Google Scholar 

  • Kuznetsov V, Shorinaa M, Aronovaa E, Stetsenkoa L, Rakitina V, Shevyakovaa N (2006) NaCl-and ethylene-dependent cadaverine accumulation and its possible protective role in the adaptation of the common ice plant to salt stress. Plant Sci. 172:363–370

    Article  Google Scholar 

  • Larcher W (2000) Ecophysiology Vegetable. RiMa. pp 531

    Google Scholar 

  • Lerslerwong L, Ketsa S, van Doorn WG (2009) Protein degradation and peptidase activity petal senescence in Dendrobium cv. Khao Sanan. Postharvest Biol Technol 52:84–90

    Article  CAS  Google Scholar 

  • Li C, Jiao J, Wang G (2004) The important roles of reactive oxygen species in the relationship between ethylene and polyamines in leaves of spring wheat seedlings under root osmotic stress. Plant Sci 166:303–315

    Article  CAS  Google Scholar 

  • Lima GPP, Broetto F, Brasil OG (1998) Salinity effect on the protein content and activities of peroxidase and nitrate reductase in rice callus. Acta Biol Leop 20:357–363

    Google Scholar 

  • Lima GPP, da Rocha SA, Takaki M, Ramos PRR, Ono EO (2008) Comparison of polyamine, phenol and flavonoid contents in plants grown under conventional and organic methods. Internat. J Food Sci Technol 43:1838–1843

    Article  CAS  Google Scholar 

  • Liu K, Fu HH, Bei QX, Luan S (2000) Inward potassium channel in guard cells as a target for polyamine regulation of stomatal movements. Plant Physiol 124:1315–1325

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Liu JH, Kitashiba H, Wang J, Ban Y, Moriguchi T (2007) Polyamines and their ability to provide environmental stress tolerance to plants. Plant Biotechnol 24:117–126

    Article  CAS  Google Scholar 

  • Macnish AJ, Reid MS, Marrero A, Jiang CZ (2009) Improving the postharvest performance of bird-of-paradise flowers. Acta Hortic 3:1763–1769

    Google Scholar 

  • Martínez-Romero D, Serrano M, Valero D (2003) Physiological changes in pepino (Solanum muricatum Ait.) fruit stored at chilling and nonchilling temperatures. Postharvest Biol Technol 30:177–186

    Article  Google Scholar 

  • Mirdehghan SH, Rahemic M, Martínez-Romero AD, Guilléna F, Valverdea JM, Zapata PJ, Serrano M, Valero D (2007) Reduction of pomegranate chilling injury during storage after heat treatment: Role of polyamines. Postharvest Biol Technol 44:19–25

    Article  CAS  Google Scholar 

  • Mukherjee D, Rao KUM (1993) Alternation pattern of Hill activity, peroxidase activity and sugars of pigeonpea during maturation and senescence. Plant Physiol Biochem 20:45–48

    Google Scholar 

  • Nowak J, Rudnicki RM, Duncan AA (1990) Postharvest handling and storage of cut flowers, florist greens and potted plant. Portland: Timber Press 20:45–48

    Google Scholar 

  • Pak C, van Doorn WG (2005). Delay of Iris flower senescence by protease inhibitors. New Phytol 165:473–480

    Article  CAS  PubMed  Google Scholar 

  • Passardi F, Penel C, Dunand C (2004) Performing the paradoxical: how plant peroxidases modify the cell wall. Trends Plant Sci 65:1879–1893

    CAS  Google Scholar 

  • Purvis AC, Shewfelt RL (1993) Does the alternative pathway ameliorate chilling injury in the sensitive plant tissues. Plant Physiol 88:712–718

    Article  CAS  Google Scholar 

  • Rani P, Singh N (2014) Senescence and Postharvest Studies of Cut Flowers: A Critical Review Pooja. Pert J Trop Agric Sci 37:59–201.

    Google Scholar 

  • Siegel BZ (1993) Plant peroxidase -an organismic perspective. Plant Growth Regul 12:303–312

    Article  CAS  Google Scholar 

  • Skutnik E, Lukaszewskaa A, Serekb M, Rabiza L (2001) Effect of growth regulators on postharvest characteristics of Zantedeschia aethiopica. Postharvest Biol Technol 21:241–246

    Article  CAS  Google Scholar 

  • Sood S, Vyas D, Nagar PK (2006) Physiological and biochemical studies during flower development in two rose species. Sci Hortic 108:390–396

    Article  CAS  Google Scholar 

  • Sugawara H, Shibuya K, Yoshioka T, Hashiba T, Satoh S (2002) Is a cysteine proteinases inhibitor involved in the regulation of petal wilting in senescing carnation (Dianthus caryophyllus L.) flowers? J Exp Bot 53:407–413

    Article  CAS  PubMed  Google Scholar 

  • Thomashow MF (2001) So what’s new in the field of plant cold acclimation. Plant Physiol 125:89–93. doi:10.1104/pp.125.1.9

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Van Doorn WG, Vaslier N (2002) Wounding-induced xylem occlusion in stems of cut chrysanthemum flowers: roles of peroxidase and catechol oxidase. Postharvest Biol Technol 26:275–284

    Article  CAS  Google Scholar 

  • Vaslier N, van Doorn WG (2003) Xylem occlusion in bouvardia flowers: evidence for a role of peroxidase and catechol oxidase. Postharvest Biol Technol 28:231–237

    Article  CAS  Google Scholar 

  • Vieira MRS, Teixeira Da Silva JA, Lima GPP, Vianello F (2010) Changes in polyamine, total protein and total carbohydrate content and peroxidase activity during the lifetime of chrysanthemum ‘Faroe’. Floric Ornam Biotechnol 4:48–52

    Google Scholar 

  • Vieira MRS, Lima GPP, Vianello F, Souza AV, Oliveira NG, Simões AN (2014) Influence of storage temperature on quality of inflorescences strelitzia. Biosci J 30:783–790

    Google Scholar 

  • Vieira MRS, Lima GPP, Vianello F, Souza AV, Simões AN, Moura FB (2015) Polyamine, peroxidase activity and total protein content during storage of bird-of-paradise. Aust J Crop Sci 9:313–320

    Google Scholar 

  • Wagstaff C, Leverentz MK, Griffiths G, Thomas B, Chanasut U, Stead AD, Rogers HJ (2002) Cysteine protease gene expression and proteolytic activity during senescence of Alstroemeria petals. J Exp Bot 53:233–240

    Article  CAS  PubMed  Google Scholar 

  • Woodson WR, Handa AK (1987) Changes in protein patterns and in vivo proteins synthesis during presenescence and senescence of hibiscus petals. J Plant Physiol 128:67–77

    Article  CAS  Google Scholar 

  • Yun DL (2007) Effects of different pretreatment preservatives and cold storage on fresh preservation of cut common callalily. Dissertation, Sichuan Agricultural University

    Google Scholar 

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Authors and Affiliations

  1. Department of Production Vegetables, Unidade Acadêmica de Serra Talhada, Universidade Federal Rural de Pernambuco, Serra Talhada-PE, Brazil

    Marcos R. da S. Vieira, Luiz G. M. Pessoa, Walter S. E. Júnior, Ygor H. Leal, Luzia F. da Silva, Rafael C. Silva, Eduardo S. dos Santos, Thialla L. Amorim & Damina C. de Medeiros

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  1. Marcos R. da S. Vieira
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  2. Luiz G. M. Pessoa
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  3. Walter S. E. Júnior
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Correspondence to Marcos R. da S. Vieira.

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Vieira, M.R.d.S., Pessoa, L.G.M., Júnior, W.S.E. et al. The effects of two preservatives on peroxidase activity and total protein content in Strelitzia reginae during cold storage. Hortic. Environ. Biotechnol. 57, 148–155 (2016). https://doi.org/10.1007/s13580-016-0109-6

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