Abstract
Chlorophyll (Chl) fluorescence analysis was used to assess stress tolerance in wild and cultivated strawberry species. We found that the parameters, photochemical quenching (1-qP), non-photochemical quenching (qN), and maximum quantum yield (Fv/Fm), can serve as stress indicators because they are sensitive to early responses to stress. The most sensitive region used for measuring Chl fluorescence in strawberry leaves was the upper surfaces of leaflets located in the middle of new leaves. An analysis of the Chl fluorescence characteristics of strawberry species showed that octoploid species had greater stress tolerance than diploid species. The ‘Whiteberry’ maintained high levels of 1-qP and qN through the dissipation of excess excitation energy as heat during early stress treatment. These results suggest that ‘Whiteberry’ has a photoinhibition system that allows it to respond to stress in a more sensitive manner than other cultivars. Therefore, among the Chl fluorescence parameters examined, 1-qP and qN, can serve as good indicators for comparing stress tolerance, and they can be used to simultaneously screen many plants for stress tolerance in strawberry breeding programs.
Similar content being viewed by others
Literature Cited
Anderson, J.M., Y.I. Park, and W.S. Chow. 1997. Photoinactivation and photoprotection of photosystem II in nature. Physiol. Plant. 100:214–223.
Apel, K. and H. Hirt. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. Physiol. 55:373–399.
Baker, N.R. and E. Rosenqvist. 2004. Applications of chlorophyll fluorescence can improve crop production strategies: An examination of future possibilities. J. Expt. Bot. 55:1607–1621.
Calatayud, A., D. Roca, and P.F. Martinez. 2006. Spatial-temporal variations in rose leaves under water stress conditions studied by chlorophyll fluorescence imaging. Plant Physiol. Biochem. 44:564–573.
Choi, J.M., M.H. Nam, and D.Y. Kim. 2012. Characterization of Toxicity symptom and determination of tissue threshold levels of boron for diagnostic criteria in domestically bred strawberries. Kor. J. Hort. Sci. Technol. 30:144–151.
Dziadczyk. P., H. Bolibok, M. Tyrka, and J.A. Hortynski. 2003. In vitro selection of strawberry (Fragaria × ananassa Duch.) clones tolerant to salt stress. Euphytica 132:49–55.
Gorbe, E. and A. Calatayud. 2012. Applications of chlorophyll fluorescence imaging technique in horticultural research: A review. Sci. Hort. 138:24–35.
Klamkowski, K. and W. Treder. 2006. Morphological and physiological responses of strawberry plants to water stress. Agri. Conspec. Sci. 71:159–165.
Krause, H. 1988. Photoinhibition of photosynthesis: An evaluation of damaging and protective mechanisms. Physiol. Plant. 74:566–574.
Lambrev, P.H., Y. Miloslavina, P. Jahns, and A.R. Holzwarth. 2012. On the relationship between non-photochemical quenching and photoprotection of photosystem II. Biochim. Biophy. Acta. 1817:760–769.
Lee, H.Y., Y.I. Park, C.G. Kim, and Y.N. Hong. 2006. Photosynthetic responses and photoprotection in Korean hot pepper (Capsicum annuum L.) against high light stress. Kor. J. Environ. Agric. 25:107–117.
Maxwell, K. and G.N. Johnson. 2000. Chlorophyll fluorescence-a practical guide. J. Expt. Bot. 51:659–668.
Muller. P., X.P. Li, and K.K. Niyogi. 2001. Non-photochemical quenching: A response to excess light energy. Plant Physiol. 125:1558–1566.
Rahimi, A., A. Biglarifard, and M. Firozabadi. 2011. Influence of NaCl salinity on some physiological aspect of strawberry cv. Camarosa. Russian Agri. Sci. 37:378–384.
Razavi, F., B. Pollet, K. Steppe, and M.C. Vanlabeke. 2008. Chlorophyll fluorescence as a tool for evaluation of drought stress in strawberry. Photosynthetica 46:631–633.
Rohacek, K., J. Soukupova, and M. Bartak. 2008. Chlorophyll fluorescence: A wonderful tool to study plant physiology and plant stress. Res. Signpost. p. 41–104.
Russell, A.W., C. Critchley, S.A. Robinson, L.A. Franklin, G.G.R. Seaton, W.S. Chow, J.M. Anderson, and C.B. Osmond. 1995. Photosystem II Regulation and dynamics of the chloroplast Di protein in Arabidopsis leaves during photosynthesis and photoinhibition. Plant Physiol. 107:943–952.
Sayed, O.H. 2003. Chlorophyll fluorescence as a tool in cereal crop research. Photosynthetica 41:321–330.
Schreiber, U., U. Schliwa, and W. Bilger. 1986. Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth. Res. 10:51–62.
Shao, L., Z. Shu, S.L. Sun, and C.L. Peng. 2007. Antioxidant of anthocyanins in photosynthesis under high temperature stress. J. Integr. Plant Biol. 49:1341–1351.
U, Z.K., S.J. Song, and U. Hansen. 1994. Stress effects on photosynthesis of greenhouse plants as measured by the fluorescence method. Kor. J. Environ. Agric. 12:183–190.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Na, YW., Jeong, H.J., Lee, SY. et al. Chlorophyll fluorescence as a diagnostic tool for abiotic stress tolerance in wild and cultivated strawberry species. Hortic. Environ. Biotechnol. 55, 280–286 (2014). https://doi.org/10.1007/s13580-014-0006-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13580-014-0006-9