Abstract
Seeds of two commercially marketable small shrubs, Ardisia crenata and Ardisia japonica, do not germinate if they are stored for more than few weeks in conditions where they are allowed to dehydrate, and they are considered as recalcitrant. The berries of these plants remain attached for a long period of time after an approximately 34 weeks period of development. The proteins in the developing seeds, germinating seeds, and seeds stored for various periods of time in moist or dry conditions and at 10°C or 25°C were examined by a polyacrylamide gel electrophoresis and an immunoblot analysis with antibodies to dehydrin and oleosin. Both dehydrin- and oleosin-like proteins were detected in early stages of seed development, as were proteins that are likely to function as seed storage proteins. Storage of seeds in dry conditions induced the expression of both dehydrin- and oleosin-like proteins, but only after an 8 weeks storage period. The levels of these proteins were considerably lower in the less dehydration tolerant A. japonica.
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Literature cited
Bailey, L.H. 1925. The standard cyclopedia of horticulture. McMillan Co., London, England.
Battaglia, M., Y. Olvera-Carrillo, A. Garciarrubio, F. Campos, and A. Covarrubias. 2008. The enigmatic LEA proteins and other hydrophilins. Plant Physiol. 148:6–24.
Berjak, P. and N.W. Pammenter. 2008. From Avicennia to Zizania: Seed recalcitrance in perspective. Ann. Bot. 101:213–228.
Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analy. Biochem. 72:248–254.
Close, T. 1996. Dehydrins: Emergence of a biochemical role of the family of plant dehydration proteins. Physiol. Plant. 97:795–803.
Farnsworth, E. 2000. The ecology and physiology of viviparous and recalcitrant seeds. Ann. Rev. Ecol. Syst. 31:107–138.
Farrant, J.M., N.W. Pammenter, P. Berjak, E.J. Farnsworth, and C.W. Vertucci. 1996. Presence of dehydrin-like proteins and levels of abscisic acid in recalcitrant (desiccation sensitive) seeds may be related to habitat. Seed Sci. Res. 6:175–182.
Finch-Savage, W.E., S.K. Pramanik, and J.D. Bewley. 1994. The expression of dehydrin proteins in dessication-sensitive (recalcitrant) seeds of temperate trees. Planta 193:478–485.
Greggains, V., W.E. Finch-Savage, W.P. Quick, and N.M. Atherton. 2000. Putative desiccation tolerance mechanisms in orthodox and recalcitrant seeds of the genus Acer. Seed Sci. Res. 10:317–327.
Hanin, M., F. Brini, C. Ebel, Y. Toda, S. Takeda, and K. Masmoudi. 2011. Plant dehydrins and stress tolerance. Versatile proteins for complex mechanisms. Plant Signaling Behavior 6:1503–509
Herman, E. 1987. Immunogold-localization and synthesis of an oil-body membrane protein in developing soybean seeds. Planta 172:336–345.
Herman, E. 1995. Cell and molecular biology of seed oil bodies, p. 195–214. In: G. Galili (ed.). Seed development and germination. Marcel Dekker, New York.
Hoekstra, F.A., E.A. Golovina, and J. Buitink. 2001. Mechanisms of plant desiccation tolerance. Trends in Plant Sci. 6:431–438.
Kermode, A.R. 1995. Regulatory mechanisms in the transition from seed development to germination: Interactions between the embryo and the seed environment, p. 273–332. In: G. Galili (ed.). Seed development and germination. Marcel Dekker, Inc., New York.
Kermode, A.R. 1997. Approaches to elucidate the basis of desiccationtolerance in seeds. Seed Sci. Res. 7:75–95
Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685.
Lee, A.-K. 1998. Ecological studies on Ardisia native to Korea and the significance as a potential indoor horticultural crop. Ph. D. Diss., Dankook University, Cheonan, Korea.
Lee, A.-K., J.-K. Suh, M.S. Roh, and J.P. Slovin. 2003. Analysis of genetic relationships of Ardisia spp. using RAPD markers. J. Hort. Sci. Biotechnol. 78:24–28.
Leprince, O., A.C. van Aelst, H.W. Pritchard, and D.J. Murphy. 1998. Oleosins prevent oil-body coalescence during seed imbibition as suggested by a low-temperature scanning electron microscope study of desiccation-tolerant and -sensitive oilseeds. Planta 204:109–119.
Levi, A., G.R. Panta, C.M. Parmentier-Line, M. Muthailif, R. Arora, S. Shanker, and L.J. Rowland. 1999. Complementary DNA cloning, sequencing and expression of an unusual dehydrin from blueberry floral buds. Physiol. Plant. 107:98–109.
McCarty, D.R. 1995. Genetic control and integration of maturation and germination pathways in seed development. Ann. Rev. Plant Physiol. Plant Mole. Biol. 46:71–93.
Moline, H.E., K.S. Johnson, and J.D. Anderson. 1983. Evaluation of two-dimensional polyacrylamide gel electrophoresis of acidic proteins of ribosome preparations for identifying plant pathogenic soft-rotting bacteria. Phytopathology 73:224–227.
Neal, J.W., J.C. Davis, J.A. Bentz, J.D. Warthen, R.J. Griesbach, and F.S. Santamour. 1998. Allelochemical activity in Ardisia species (Myrsinaceae) against selected arthropods. J. Economic Entomol. 91:608–617.
Newell, A.M.B., G.G. Yousef, M.A. Lila, M.V. Ramires-Mares, and E. Gonzalez de Mejia. 2010. Comparative in vitro bioactivities of tea extracts from six species of Ardisia and their effect on growth inhibition of HepG2 cells. J. Ethnopharmacol. 130:536–544.
Pammenter, N.W. and P. Berjak. 1999. A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms. Seed Sci. Res. 9:13–37.
Parthibane, V., S. Rajakumari, V. Ventkateshwari, R. Iyappan, and R. Rajasekharan. 2012. Oleosin is bifunctional enzyme that has both monoacylglycerol acyltransferase and phospholipase activities. J. Biol. Chem. 287:1946–1954.
Roberts, E.H. 1973. Predicting the storage life of seeds. Seed Sci. Technol. 1:499–514.
Sumino, M., T. Sekine, N. Ruangrungsi, K. Igarashi, and F. Ikegami. 2000. Ardisiophenols and other antioxidant principles from the fruits of Ardisia colorata. Chem. Pharm. Bull. 50:1484–1487.
Sun, W.Q. and Y.H. Liang. 2001. Discrete levels of dessiccation sensitivity in various seeds as determined by the equilibrium dehydration method. Seed Sci. Res. 11:317–323.
Tweddle, J.C., J.B. Dickie, C.C. Baskin, and J.M. Baskin. 2003. Ecological aspects of seed desiccation sensitivity. J. Ecol. 91: 294–304.
Yang, I.S. and W. Kim. 1973. Conspectus relation between the distribution of evergreen broad-leaved trees and the climatic factor in the Southern area of Korea. J. Kor. Plant Taxon. 4:11–18.
Yang, Q.H., Q.Y. Lan, H.S. Yang, Y.H. Tan, and W.H. Ye. 2011. Germination, desiccation, storage and germination-accelerating pretreatment of Ardisia virens seeds. Seed Sci. Technol. 39:327–337.
Yang, Q.H., W.H. Ye, Z.M. Wang, and X.J. Yin. 2009. Seed germination physiology of Ardisia crenata var. bicolor. Seed Sci. Technol. 37:291–302.
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Lee, A.K., Slovin, J.P. & Suh, J.K. Dehydration intolerant seeds of Ardisia species accumulate storage and stress proteins during development. Hortic. Environ. Biotechnol. 53, 530–538 (2012). https://doi.org/10.1007/s13580-012-0073-8
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DOI: https://doi.org/10.1007/s13580-012-0073-8