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Screening different methods of tetraploid induction in watermelon [Citrullus lanatus (thunb.) Manst. and Nakai]

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Abstract

To screen a suitable method for inducing tetraploid with high frequency, five diploid inbred lines of watermelon, WC-8C-2C, WD-2-6K, GW11, GW7, and GW2 were selected. The efficiency of chromosome doubling with three methods direct seed (DS), shoot apex (SA), and inverted hypocotyl (IH) were tested by applying two different concentrations (0.1% and 0.2%) of antimitotic agent, colchicine, during the period January, 2010 to August, 2011 at Watermelon Experiment Station, Daesan, Gochang, Jeonbuk, South Korea. Mean chloroplast counts were 12 and 19 in diploid and tetraploid, respectively. The highest rate of efficiency of generating tetraploids (29.5%) was recorded in 0.2% IH method and considered as s best method. A total of 71 new tetraploids were generated successfully using present methods and techniques. WC-8C-2C and GW7 produced maximum number of tetraploids.

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Authors and Affiliations

  1. Watermelon Experiment Station, Jeollabuk-do Agricultural Research & Extension Services, Gochang, 585-863, Korea

    Jaejong Noh, Sameena Sheikh, Hyong Gwon Chon, Mun Ho Seong, Jeong Hyeon Lim, So Gun Lee & Gi Tai Jung

  2. Specialization Crop Research Institute, Jeollabuk-do Agricultural Research & Extension Services, Jinan, 567-807, Korea

    Jeong Man Kim

  3. Department of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, 561-756, Korea

    Ho-Jong Ju

  4. National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon, 440-706, Korea

    Yun Chan Huh

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  1. Jaejong Noh
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  2. Sameena Sheikh
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  3. Hyong Gwon Chon
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  4. Mun Ho Seong
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Correspondence to Jaejong Noh.

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Noh, J., Sheikh, S., Chon, H.G. et al. Screening different methods of tetraploid induction in watermelon [Citrullus lanatus (thunb.) Manst. and Nakai]. Hortic. Environ. Biotechnol. 53, 521–529 (2012). https://doi.org/10.1007/s13580-012-0055-x

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  • DOI: https://doi.org/10.1007/s13580-012-0055-x

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