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Development of gene-based markers for the Bs2 bacterial spot resistance gene for marker-assisted selection in pepper (Capsicum spp.)

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Abstract

The Bs2 gene of pepper (Capsicum spp.) confers resistance to the most commonly detected races of Xanthomonas campestris pv vesicatora (Xcv). Using information from a published database, we developed gene-based markers for selection of Bs2 in pepper. High polymorphism was detected in exon 3, intron 2 and 3’-UTR of the Bs2 gene. The three regions were sequenced and numerous SNPs and In/Dels were identified between resistant and susceptible inbred lines. Seven SNPs were selected to develop SNP markers using the tetra-primer amplification refractory mutation system-PCR methodology (Tetra-primer ARMS-PCR). One SNP (G/C) was used to develop a molecular marker that can perfectly identify the presence of the bacterial spot resistance. The gene-based markers were further validated to test association with resistance in 80 lines and cultivars. The markers successfully identified a bell pepper commercial cultivar 62-2 that harbors the Bs2 gene. The successful development of the markers in this study provides a useful tool for MAS to screen the Bs2 gene in pepper.

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Authors and Affiliations

  1. Vegetable Research Division, Department of Horticultural Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, Suwon, 441-440, Korea

    Hai Thi Hong Truong, Ki-Taek Kim, Su Kim, Myoung-Cheol Cho, Hyun-Ran Kim & Jong-Gyu Woo

  2. Department of Horticulture Science, Agronomy Faculty, Hue University of Agriculture and Forestry, 102 Phung Hung, Hue, Vietnam

    Hai Thi Hong Truong

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  1. Hai Thi Hong Truong
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  2. Ki-Taek Kim
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  3. Su Kim
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  4. Myoung-Cheol Cho
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  5. Hyun-Ran Kim
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  6. Jong-Gyu Woo
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Correspondence to Ki-Taek Kim.

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Truong, H.T.H., Kim, KT., Kim, S. et al. Development of gene-based markers for the Bs2 bacterial spot resistance gene for marker-assisted selection in pepper (Capsicum spp.). Hortic. Environ. Biotechnol. 52, 65–73 (2011). https://doi.org/10.1007/s13580-011-0142-4

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  • DOI: https://doi.org/10.1007/s13580-011-0142-4

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