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pCtFSG2, a flower-specific promoter with suitable promoter activity in safflower

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Abstract

Safflower (Carthamus tincorius L.) is an economically important plant. Flowers are a source of dyes and active ingredients in medicines. The development of flower-specific promoters is of great significance for the directed regulation of the biosynthesis of targeted products. However, few flower-specific promoters have been reported in safflower. In this study, transcriptome analysis of various safflower tissues was performed. A total of 40.37 Gb of clean data were obtained, and 100 safflower-specific genes were screened. Three genes (CtFSG1, CtFSG2 and CtFSG3) were identified by RT–PCR, and all had high expression in flowers. The promoters of the three genes (pCtFSG1, pCtFSG2 and pCtFSG3) were cloned, and the promoter sequences were analysed. Promoter activity analysis showed that pCtFSG2 had suitable promoter activity in protoplasts of tubiform floret in safflower. This promoter specificity was further characterized by Agrobacterium-mediated transformation of pCtFSG2::GUS to Arabidopsis thaliana. The results showed that GUS staining was detectable only in flowers. Moreover, deletion analysis of the pCtFSG2 promoter region revealed that the DNA segments from − 1953 to − 1534 and − 980 to − 490 are critical for the expression of pCtFSG2. Our results indicated that pCtFSG2 is a flower-specific promoter with suitable promoter activity in safflower, which has potential value for directional modification of safflower.

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Abbreviations

GUS:

β-Glucuronidase

CHS :

Chalcone synthase gene

CtFSG :

Flower-specific genes in safflower (Carthamustincorius L.)

SON-PCR:

Single oligonucleotide nested PCR

CDS:

Coding sequence

LUC:

Luciferase

SRA:

Sequence read archive

qRT–PCR:

Real-time quantitative RT–PCR

DAA:

Day after anthesis

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Acknowledgements

We acknowledge Dr Jianlan Zhou (Biomarker Biological Technology Co., Ltd, Beijing) for the data analysis and American Journal Experts (AJE) for language editing. This work was supported by the National Natural Science Foundation of China (Nos: 82274039, U19A2010), the China Postdoctoral Science Foundation (203638), National Multidisciplinary Interdisciplinary Innovation Team Project of Traditional Chinese Medicine (ZYYCXTD-D-202209) and Sichuan Province’s “14th Five-Year Plan” crop and livestock breeding research project (2021YFYZ0012-5).

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Authors and Affiliations

  1. State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, Sichuan, China

    Jiang Chen, Chaoxiang Ren, Qinghua Wu & Jin Pei

  2. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, Sichuan, China

    Jiang Chen, Chaoxiang Ren, Jie Wang, Bin Xian, Rui Wang, Qinghua Wu & Jin Pei

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  1. Jiang Chen
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  2. Chaoxiang Ren
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  3. Jie Wang
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  5. Rui Wang
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  7. Jin Pei
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Correspondence to Jin Pei.

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Chen, J., Ren, C., Wang, J. et al. pCtFSG2, a flower-specific promoter with suitable promoter activity in safflower. J. Plant Biochem. Biotechnol. 32, 478–486 (2023). https://doi.org/10.1007/s13562-023-00829-1

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