Abstract
The aim of this investigation was to study the effect of physical (ultraviolet light) and chemical mutagen (methyl salicylate) on common bean (Phaseolus vulgaris L.) and how they had positive or negative effect on alpha-amylase activity, phenological plant traits (days to flowering, days to maturity, days of pod fill) and agronomic traits (number of pods per plant; weight of pods per plant; Number of seeds per pod; number of seeds per plant; weight of seeds per plant; plant height; pod harvest index), seed and pod morphological characteristics (seed length; seed width; pod length; pod width). Seeds of common bean were exposed to ultraviolet (UV) light for different periods of exposure time (3 and 5 h) and were soaked with two different methyl salicylate (MeSA) concentrations (0.01 and 0.05 mM). The results indicated that the 5 h exposure to UV light plants were indicated to positively affect alpha-amylase activity, phenological and agronomic traits, seed and pod morphological characteristics as compared to control and other treatments while 0.01 mM MeSA significantly negatively impacted them. Traits of plants exposed to UV light for 5 h were significantly improved. This research could provide a valuable source of materials for common bean breeding.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- MeSA:
-
Methyl salicylate
- UV:
-
Ultraviolet light
- SA:
-
Salicylic acid
- UV-3 h:
-
Seeds were treated with UV light in 3 h
- UV-5 h:
-
Seeds were treated with UV light in 5 h
- MeSA-0.01:
-
Seeds were treated with MeSA at 0.01 mM concentrations
- MeSA-0.05:
-
Seeds were treated with MeSA at 0.05 mM concentrations
References
Acosta-Gallegos JA, Kelly JD, Gepts P (2007) Pre-Breeding in common bean and use of genetic diversity from wild germplasm. Crop Sci 47:44–59. https://doi.org/10.2135/cropsci2007.04.0008ipbs
Ahmed MA, Chakraborty N (2017) The effect of physical and chemical mutagen on tomato plant. Adv Biol Res 11:64–69. https://doi.org/10.5829/idosi.abr.2017.64.69
Ali HMA, Shah SA (2013) Evaluation and selection of rapeseed (Brassica napus L.) mutant lines for yield performance using augmented design. J Anim Plant Sci 23(4):1125–1130
Asfaw A, Ambachew D, Shah T, Blair MW (2017) Trait associations in diversity panels of the two common bean (Phaseolus vulgaris L.) gene pools grown under well-watered and water-stress conditions. Front Plant Sci 8:733. https://doi.org/10.3389/fpls.2017.00733
Babak P, Mohammad RS (2012) UV Irradiation effects on seed germination and growth, protein content, peroxidase, and protease activity in red bean. Int J Eng Sci 1(3):107–113
Bandurska H, Niedziela J, Chadzinikolau T (2013) Separate and combined responses to water deficit and UV- B radiation. Plant Sci 213:98–105. https://doi.org/10.1016/j.plantsci.2013.09.003
Bashir S, Wani AA, Nawchoo IA (2013) Mutagenic sensitivity of gamma rays EMS and sodium azide in trigonella foenum-graecum L. Sci Res Report 3(1):20–26
Beebe SE (2012) Common bean breeding in the tropics. Plant Breed Rev 36:357–426. https://doi.org/10.1002/9781118358566.ch5
Boschin G, Arnoldi A (2011) Legumes are valuable sources of tocopherols. Food Chem 127:1199–1203. https://doi.org/10.1016/j.foodchem.2011.01.124
Broughton WJ, Hernández G, Blair M, Beebe S, Gepts P, Vanderleyden J (2003) Beans (Phaseolus spp.)-model food legumes. Plant Soil 252:55–128. https://doi.org/10.1023/A:1024146710611
Dai Q, Peng S, Chavez AQ, Vergara BS (1995) Effects of UV-B radiation on stomatal density and opening in rice (Oryza sativa L.). Ann Bot 76:65–70. https://doi.org/10.1006/anbo.1995.1079
Deckmyn G, Impens I (1998) Effects of solar UV-B irradiation on vegetative and generative growth of Bromus catharticus. Environ Exp Bot 40:179–185. https://doi.org/10.1016/S0098-8472(98)00035-5
Devi AS, Mullainathan L (2011) Physical and chemical mutagenesis for improvement of chilli (Capsicum annuum L.). World Appl Sci J 15:108–113
Dhakshanamoorthy D, Selvaraj R, Chidambaran A (2010) Physical and chemical mutagenesis in Jatropha Curcas Lto induce variability in seed germination, growth and yield traits. Rom J Biol Plant Biol 55:113–125
Frohnmeyer H, Staiger D (2003) Ultraviolet-B radiation-mediated responses in plants. Balancing damage and protection. Plant Physiol 133:1420–1428. https://doi.org/10.1104/pp.103.030049
Ha PTT, Dac HV, Thu NNM, Tram NTN, Khang LM, Ngoc PDB (2019) Effect of Methyl Salicylate (MeSA) mutagenesis in mustard brassica (Brassica juncea) under salinity stress. Indian Hortic J 9:13–21
Ha PTT, Tra NT, Nguyen NDT, Khang LM, Khang DT (2020) Evaluate the effects of salt stress on physico-chemical characteristics in the germination of rice (Oryza sativa L.) in response to methyl salicylate (MeSA). Biocatal Agric Biotechnol 23:101470. https://doi.org/10.1016/j.bcab.2019.101470
Jabeen N, Mirza B (2004) Ethyl Methane Sulfonate induces morphological mutations in Capsicum annuum. Int J Agri Biol 6:340–345
Kalaivani K, Kalaiselvi MM, Senthil-Nathan S (2016) Effect of methyl salicylate (MeSA), an elicitor on growth, physiology and pathology of resistant and susceptible rice varieties. Sci Rep 6:34498. https://doi.org/10.1038/srep34498
Koo YM, Heo AY, Choi HW (2020) Salicylic acid as a safe plant protector and growth regulator. Plant Pathol J 36:1–10. https://doi.org/10.5423/PPJ.RW.12.2019.0295
Lal GM, Toms B, Lal SS (2009) Mutagenic sensitivity in early generation in blackgram. Asian J Agric Res 1:9–11
Lee YH, Park W, Kim KS, Jang YS, Lee JE, Cha YL, Moon YH, Song YS, Lee K (2018) EMS-induced mutation of an endoplasmic reticulum oleate desaturase gene (FAD2- 2) results in elevated oleic acid content in rapeseed (Brassica napus L.). Euphytica 214:28. https://doi.org/10.1007/s10681-017-2106-y
Mowaffaq AA, Nandita C, Yasser T, Saad SD, Ida SI, Raffeezul M, Muhammad AY, Doblin S (2017) The Effect of physical and chemical mutagen on tomato plant. Adv Biol Res 11(2):64–69. https://doi.org/10.5829/idosi.abr.2017.64.69
Parry MA, Madgwick PJ, Bayon C, Tearall K, Hernandez-Lopez A, Baudo M, Rakszegi M, Hamada W, Al-Yassin A, Ouabbou H, Labhilili M, Phillips AL (2009) Mutation discovery for crop improvement. J Exp Bot 60(10):2817–2825. https://doi.org/10.1093/jxb/erp189
Reddy KVG, Geetha RR (2013) Classical ragas: a new protein supplement in plants. Indian J Life Sci 3:97–103
Reyes-Bastidas M, Reyes-Fernandez EZ, Lopez-Cervantes J, Milan-Carrillo J, Loarca-Pina GF, Reyes-Moreno C (2010) Physicochemical, nutritional and antioxidant properties of tempeh flour from common bean (Phaseolus vulgaris L.). Food Sci Technol Int 16:427–434. https://doi.org/10.1177/1082013210367559
Ries G, Heller W, Puchta H, Sandermann H, Seidlitz HK, Hohn B (2000) Elevated UV-B radiation reduces genome stability in plants. Nature 406:98–101. https://doi.org/10.1038/35017595
Rondini EA, Barrett KG, Bennink MR (2012) Nutrition and human health benefits of dry beans and pulses. Dry Beans and Pulses Prod Process Nutr. https://doi.org/10.1002/9781118448298.ch14
Saile-Mark M, Tevini M (1997) Effects of solar UV-B radiation on growth, flowering and yield of central and southern European bush bean cultivars (Phaseolus vulgaris L.). Plant Ecol 128:115–125. https://doi.org/10.1007/978-94-011-5718-6_11
Saile-Mark M, Tevini M, Mark U (2008) Effects of solar UVB radiation on growth, flowering, and yield of central and southern European maize cultivars (Zea mays L.). Photochem Photobiol 64:457–463. https://doi.org/10.1111/j.1751-1097.1996.tb03091.x
Salama HM, Al Watban AA, Al-Fughom AT (2011) Effect of ultraviolet radiation on chlorophyll, carotenoid, protein and proline contents of some annual desert plants. Saudi J Biol Sci 18:79–86. https://doi.org/10.1016/j.sjbs.2010.10.002
Schnurbush T, Mollers C, Becker HC (2000) A mutant of B. napus with increased palmitic acid content. Plant Breed 119:141–144. https://doi.org/10.1046/j.1439-0523.2000.00481.x
Scully BT, Wallace DH (1990) Variation in and relationship of biomass, growth rate, harvest index, and phenology to yield of common bean. J Am Soc Hortic Sci 115:218–225. https://doi.org/10.21273/JASHS.115.2.218
Shaukat SS, Zaidi S, Khan MA (2011) Effect of supplemental UV-B radiation on germination seedling growth, and biochemical responses of sunflower (Helianthus annuus L.). FUUAST J Biol 1:27–33
Simonova EN, Ayrapetyan MM, Eroshenko AA, Vertiy NS (2019) The Impact of UV irradiation of winter wheat seeds on enzymatic activity in the germination period. Indian J Agric Sci 54:232–236. https://doi.org/10.18805/IJARe.A-467
Souza TLPO, Faleiro FG, Dessaune SN, Paula-Junior TJ, Moreira MA, Barros EG (2013) Breeding for common bean (Phaseolus vulgaris L.) rust resistance in Brazil. Trop Plant Pathol 38:361–374. https://doi.org/10.1590/S1982-56762013005000027
Teramura AH, Sullivan JH, Lydon J (1990) Effects of UV-B radiation on soybean yield and seed quality: a 6-year field study. Physiol Plant 80:5–11. https://doi.org/10.1111/j.1399-3054.1990.tb04367.x
Tharanathan RN, Mahadevamma S (2003) Grain legumes: a boon to human nutrition. Trends Food Sci Tech 14:507–518. https://doi.org/10.1016/j.tifs.2003.07.002
Weih M, Johanson U, Gwynn-Jones D (1998) Growth and nitrogen utilization in seedlings of mountain birch (Betula pubescens ssp. tortuosa ) as affected by ultraviolet radiation (UV-A and UV-B) under laboratory and outdoor conditions. TreesStruct Funct 12:201–207. https://doi.org/10.1007/PL00009711
Yaldagard M, Mortazavi SA, Tabatabaie F (2008) The effect of ultrasound in combination with thermal treatment on the germinated barley’s alpha-amylase activity. Korean J Chem Eng 25:517–523. https://doi.org/10.1007/s11814-008-0087-1
Yaqoob M, Rashid R (2001) Induced mutation studies in some mung bean (Vigna radiata L.) Wilczek cultivars. Online J Biol Sci 1:805–808. https://doi.org/10.3923/jbs.2001.805.808
Zargar SM, Nazir M, Gupta N, Farhat S, Mahajan R, Salgotra RK, Rakwal R, Agrawal GK, Mir RA (2014) Molecular marker assisted approaches (MMAA) for enhancing low water stress tolerance in common bean: an update. Mol Plant Breed 5:1–12. https://doi.org/10.5376/mpb.2014.05.0014
Zhang Y, Peng D, Song JC, Ji J, Wang G, Li X, Guan C (2020) Enhancement of methyl salicylate accumulation promotes early flowering in transgenic tobacco plants by overexpressing a carboxymethyl transferase (SAMT) gene from Lycium chinense. Mol Breed 40:52. https://doi.org/10.1007/s11032-020-01131-x
Zlatev ZS, Lidon FJC, Kaimakanova M (2012) Plant physiological responses to UV-B radiation. Emir J Food Agric 24:481–501. https://doi.org/10.9755/ejfa.v24i6.481501
Zu Y, Pang HH, Yu JH, Li DW, Wei XX, Gao YX, Tong L (2010) Responses in the morphology, physiology and biochemistry of Taxus chinensis var. mairei grown under supplementary UV-B radiation. J Photochem Photobiol B 98:152–158. https://doi.org/10.1016/j.jphotobiol.2009.12.001
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Ha, P.T.T., Tran, N.T.B., Tram, N.T.N. et al. Effects of ultraviolet light and methyl salicylate on some phenological and agronomic and morphological characteristics of common bean (Phaseolus vulgaris L.). J. Plant Biochem. Biotechnol. 31, 907–914 (2022). https://doi.org/10.1007/s13562-022-00769-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13562-022-00769-2