Skip to main content
SpringerLink
Log in

Characterization of interspecific hybrids of Triticum aestivum x Aegilops sp. without 5B chromosome for induced homoeologous pairing

  • Short Communication
  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

In the present study we aimed to characterize the interspecific hybrids made between Triticum aestivum cv. Pavon monosomic for chromosome 5B with different accessions of Aegilops kotschyi (UUSS) and Aegilops peregrina (UUSS) at cytological, molecular and morphological basis. Molecular analysis using Ph1 locus specific dominant marker and cytological analysis clearly differentiated between F1 hybrids (ABDUS) with and without 5B chromosome. Plants without chromosome 5B showed stunted and bushy growth habit with reduced height and more number of tillers per plant while those with 5B plants showed normal growth. Agreement of morphological observations with the cytological and molecular results indicates that the morphological characteristics could also be used to screen plants without 5B chromosome. The results clearly demonstrated that the absence of chromosome 5B through the use of Pavon monosomic for chromosome 5B can be an efficient way to induce homoeologous pairing between chromosomes of wheat and Aegilops species for precise introgression of useful variability.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Abbreviations

PCR:

Polymerase chain reaction

PMCs:

Pollen mother cells

CTAB:

Cetyl trimethylammonium bromide

References

  • Ayala M, Guzmán C, Alvarez JB, Pena RJ (2013) Characterization of genetic diversity of puroindoline genes in mexican wheat landraces. Euphytica 190:53–63

    Article  Google Scholar 

  • Baum M, Lagudah ES, Appels R (1992) Wild crosses in cereals. Annu Rev Plant Phys 43:117–143

    Article  Google Scholar 

  • Cai X, Chen PD, Xu SS, Oliver RE, Chen X (2005) Utilization of alien genes to enhance Fusarium head blight resistance in wheat-A review. Euphytica 142:309–318

    Article  Google Scholar 

  • Distelfield A, Cakmak I, Peleg Z, Ozturk L, Yazici AM, Saranga Y, Fahima T (2007) Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations. Physiol Plantarum 129:635–643

    Article  Google Scholar 

  • Dyck PL, Kerber ER, Marters JW (1990) Transfer of a gene for stem rust resistance from Aegilops caudata to common wheat. Can J Plant Sci 70:931–34

    Article  Google Scholar 

  • Gill KS, Gill BS, Endo TR, Mukai Y (1993) Fine physical mapping of Ph1, a chromosome pairing regulator gene in polyploid wheat. Genetics 134:1231–1236

    PubMed  PubMed Central  Google Scholar 

  • Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73:199–212

    Article  Google Scholar 

  • Koebner RMD, Shepherd KW (1986) Controlled introgression to wheat of genes from rye chromosome arm 1RS by induction of allosyndesis, 1. Isolation of recombinants. Theor Appl Genet 73:197–208

    Article  PubMed  Google Scholar 

  • Liu Z, Sun Q, Ni Z, Nevo E, Yang T (2002) Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica 123:21–29

    Article  Google Scholar 

  • Qi L, Friebe B, Zhang P, Gill BS (2007) Homoeologous recombination, chromosome engineering and crop improvement. Chromosome Res 15:3–19

    Article  PubMed  Google Scholar 

  • Qu LJ, Foote TN, Roberts MA, Money TA, Aragon-Alcaide L (1998) A simple PCR-based method for scoring the ph1b deletion in wheat. Theor Appl Genet 96:371–375

    Article  PubMed  Google Scholar 

  • Rawat N, Tiwari VK, Singh N, Randhawa GS, Singh K, Chhuneja P, Dhaliwal HS (2009) Evaluation and utilization of Aegilops and wild Triticum species for enhancing iron and zinc content in wheat. Genet Resour Crop Ev 56:53–65

    Article  Google Scholar 

  • Riley R, Chapman V (1958) Genetic control of the cytogenetically diploid behaviour of hexaploid wheat. Nature 182:713–715

    Article  Google Scholar 

  • Riley R, Kimber G, Chapman V (1961) The origin of genetic control of diploid like behaviour of polyploid wheat. J Hered 52:22–25

    Google Scholar 

  • Riley R, Chapman V, Johnson R (1968) The incorporation of alien disease resistance in wheat by genetic interference with the regulation of meiotic chromosome synapsis. Genet Res 12:199–219

    Article  Google Scholar 

  • Schneider A, Molnár I, Molnár-Láng M (2008) Utilization of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat. Euphytica 163:1–19

    Article  Google Scholar 

  • Sears ER (1972) Agropyron-wheat transfers through induced homoeologous pairing. Can J Genet Cytol 14:736

    Google Scholar 

  • Sears ER (1977) An induced mutant with homoeologous pairing in common wheat. Can J Genet Cytol 19:585–593

    Article  Google Scholar 

  • Vasil IK (2003) The science and politics of plant biotechnology—a personal perspective. Nat Biotechnol 21:849–851

    Article  PubMed  Google Scholar 

Download references

Author information

Author notes

    Authors and Affiliations

    1. Akal College of Agriculture, Eternal University, Sirmour, Himachal Pradesh, India

      Imran Sheikh, Prachi Sharma, Dharmendra Singh, Vishal Chugh & Harcharan Singh Dhaliwal

    2. Department of Biotechnology, Indian Institute of Technology, Roorkee, Uttarakhand, India

      Shailender Kumar Verma & Satish Kumar

    3. Department of Molecular Biology and Genetic Engineering, CBSH, GB Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India

      Sachin Malik, Priyanka Mathpal, Upendra Kumar & Sundip Kumar

    Authors
    1. Imran Sheikh
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Prachi Sharma
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Shailender Kumar Verma
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Satish Kumar
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Sachin Malik
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Priyanka Mathpal
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Upendra Kumar
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Dharmendra Singh
      View author publications

      You can also search for this author in PubMed Google Scholar

    9. Sundip Kumar
      View author publications

      You can also search for this author in PubMed Google Scholar

    10. Vishal Chugh
      View author publications

      You can also search for this author in PubMed Google Scholar

    11. Harcharan Singh Dhaliwal
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Vishal Chugh.

    Additional information

    Imran Sheikh and Prachi Sharma contributed equally to this work.

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Sheikh, I., Sharma, P., Verma, S.K. et al. Characterization of interspecific hybrids of Triticum aestivum x Aegilops sp. without 5B chromosome for induced homoeologous pairing. J. Plant Biochem. Biotechnol. 25, 117–120 (2016). https://doi.org/10.1007/s13562-015-0307-9

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s13562-015-0307-9

    Keywords

    Search

    Navigation