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Differentiating tomato leaf curl viruses possessing mono/bi partite genomes using replicase gene based PCR assay: implications for developing virus specific resistance

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Abstract

Tomato leaf curl disease (ToLCD) is associated with begomoviruses, with their generic names as Tomato leaf curl viruses (ToLCVs) and Tomato yellow leaf curl viruses (TYLCVs). ToLCVs exist both with mono/bipartite genomes, whereas TYLCVs carry monopartite genomes. For management of these viruses, three resistance loci Ty-1 to Ty-3 are being deployed, which provides different degree of resistance. These viruses encode a replication initiator protein (Rep), indispensible for virus replication. The conserved/divergent sequences of rep gene(s) were used to develop mono/bipartite virus specific PCR assay. The implications of distinguishing viruses with mono/bipartite genomes for using virus specific resistance loci through breeding have been discussed.

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Fig. 1
Fig. 2

Abbreviations

ToLCD:

Tomato leaf curl disease

ToLCV:

Tomato leaf curl virus

TYLCV:

Tomato yellow leaf curl virus

Rep/rep :

Replication initiator protein/gene

RCR:

Rolling circle replication

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Acknowledgments

This work was supported by ICAR funding under NPTC project on transgenic tomato. We are also thankful to Department of Biotechnology (BIRAC) under BIPP program, Government of India for providing fellowship to Varsha Garg and Vipin Permar. SP is thankful to VPP program of KSU.

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Authors and Affiliations

  1. Advanced Center for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Varsha Garg, Vipin Permar & Shelly Praveen

  2. Advanta Ltd., Krishnama House, Road No.7, Banjara Hills, Hyderabad, 500034, India

    Vilas Parkhi & Elangovan Mani

  3. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Abdulrahman A. Alatar & Mohammad Faisal

Authors
  1. Varsha Garg
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  2. Vipin Permar
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  3. Vilas Parkhi
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  4. Elangovan Mani
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  5. Abdulrahman A. Alatar
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  6. Mohammad Faisal
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  7. Shelly Praveen
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Corresponding author

Correspondence to Shelly Praveen.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Table 1

List of 20 viruses (ToLCVs /TYLCVs) used in the present study for designing primers capable of distinguishing viruses having mono/bipartite genomes. (DOCX 14 kb)

Supplementary Table 2

List of viral cultures with their accession numbers for validation of primers. (DOCX 17 kb)

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Garg, V., Permar, V., Parkhi, V. et al. Differentiating tomato leaf curl viruses possessing mono/bi partite genomes using replicase gene based PCR assay: implications for developing virus specific resistance. J. Plant Biochem. Biotechnol. 24, 461–465 (2015). https://doi.org/10.1007/s13562-015-0299-5

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