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Effectiveness of hesperidin against toxic effect of silver nanoparticles on the brain via apoptotic/antiapoptotic pathway in adult albino rats

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Abstract

Objective

The existing work aims to assess the role of hesperidin against silver oxide nanoparticles-induced toxic changes in brain of adult albino rats.

Methods

50 adult male albino rats were divided into four groups: group Ia (−ve control); group Ib (+ve control; saline group); group II (hesperidin (HN); each rat received 50 mg/kg/day orally); group III (AgNPs; each rat received 1 mg/kg/day IP); and group IV (HN and AgNPs in the previous doses) all for 28 days. Then rats were anesthetized and killed, and specimens from the brain were taken for biochemical and histopathological studies.

Results

AgNPs exposure showed significantly increased tissue malondialdehyde (MDA), inducible nitric oxide synthase, caspase 8 and caspase 3 activities with significant depletions of superoxide dismutase activities. This was accompanied with alteration and damage in the histological structure of the brain. These changes were improved in the group receiving HN with AgNPs.

Conclusion

The results of this study showed that hesperidin had protective effects against AgNPs-induced toxicity in rat brain.

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Acknowledgements

Thanks are due to all the staff work at the animal house of Zagazig Faculty of Medicine and the staff members of the medical histology and cell biology department.

Funding

This study had no special funding from any organization.

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Authors and Affiliations

  1. Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Abeer Ramzy Hussieny Mahmoud & Nashwa Mohamad Mohamad Shalaby

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  1. Abeer Ramzy Hussieny Mahmoud
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  2. Nashwa Mohamad Mohamad Shalaby
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Contributions

All authors provided a contribution to the conception and design or data analysis in drafting or revising the manuscript.

Corresponding author

Correspondence to Abeer Ramzy Hussieny Mahmoud.

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Conflict of interest

Abeer Ramzy Hussieny Mahmoud and Nashwa Mohamad Mohamad Shalaby declare that we have no conflict of interest.

Ethical approval

The experimental work was done according to the basic instructions advised through the Institutional Research Board for the use and care of experimental animals and approved by The Institutional Animal Care and Use Committee, Zagazig University, with approval code ZU-IACUC/ 3/F/260/2022.

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Mahmoud, A.R.H., Shalaby, N.M.M. Effectiveness of hesperidin against toxic effect of silver nanoparticles on the brain via apoptotic/antiapoptotic pathway in adult albino rats. Toxicol. Environ. Health Sci. 15, 119–125 (2023). https://doi.org/10.1007/s13530-022-00165-2

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