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Pineapple peel as alternative substrate for bacterial nanocellulose production

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Abstract

Due to its flexible properties, bacterial nanocellulose (BNC) has been attracting tremendous attention. In this study, BNC was produced by Acetobacter xylinum ATCC2376 and a local isolate, namely, Bacillus cereus MMS1. The production of BNC was done by utilising pineapple peel extract (PPE) (wastes discarded after cutting the fruit) as the alternative carbon source substituting the commercial D-glucose (control) in Hestrin–Schramm (HS) medium under agitated conditions. This research is aimed to investigate the synthesis of BNC by an isolated bacterial strain from termite’s gut using an agro-industrial waste which is the pineapple peel extract. Six bacterial strains, namely, F8, F5, M1, M6, H7 and H11, were screened and identified for potential BNC producer. The selected bacterial strain was identified as Bacillus cereus MMS1 using 16S rRNA nucleotide sequences. Then, the production of BNC was done by B. cereus MMS1 using pineapple peel extract, while A. xylinum ATCC2376 acted as a control. The BNC production in this study was attained at 2% (w/v) glucose concentration, 12 days of incubation period and 150 rpm agitation speed which was 5.83 g/L by A. xylinum ATCC2376 in HS medium using commercial glucose as carbon source. Meanwhile, 4.42 g/L and 2 g/L of BNC were produced by B. cereus MMS1 after 12 days of incubation with an initial concentration of 2% (w/v) using commercial glucose and pineapple peel extract, respectively.

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Data availability

The datasets generated during and/or analysed during the current study are available in the manuscript itself.

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Acknowledgements

The authors would like to express their gratitude to AlafPutra Biowealth Sdn Bhd, Kota Tinggi, Johor, for the collaboration and supply of samples.

Funding

This research was funded by the Universiti Putra Malaysia under Putra Grant Scheme (UPM/700–2/1/Geran Putra — 959700).

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Authors and Affiliations

  1. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Adriana Connie Lee, Mohamad Faizal Ibrahim, Ezyana Kamal Bahrin, Mohd Azwan Jenol & Suraini Abd-Aziz

  2. Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia

    Madihah Md Salleh

Authors
  1. Adriana Connie Lee
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  2. Madihah Md Salleh
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  6. Suraini Abd-Aziz
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Contributions

All authors contributed to the study conception and design. Adriana Connie Lee, data collection and data analysis; Adriana Connie Lee and Suraini Abd-Aziz, data collection, data analysis and interpretation and drafting the article. Adriana Connie Lee, Madihah Md Salleh, Mohamad Faizal Ibrahim, Ezyana Kamal Bahrin and Suraini Abd-Aziz, conception or design of the work, critical revision of the article and final approval of the version to be published. All authors read and approved the final manuscript.

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Correspondence to Suraini Abd-Aziz.

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Lee, A.C., Salleh, M.M., Ibrahim, M.F. et al. Pineapple peel as alternative substrate for bacterial nanocellulose production. Biomass Conv. Bioref. 14, 5541–5549 (2024). https://doi.org/10.1007/s13399-022-03169-7

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