Abstract
The screening of new strains of high-efficiency ammonia–nitrogen degrading bacteria and the research on the treatment of high-concentration ammonia–nitrogen livestock and poultry wastewater by immobilized complex flora is still lacking, especially the research on different ratios between complex flora and operating factors. In this experiment, a new strain of ammonia–nitrogen degrading bacteria was screened from activated sludge. The species was determined by ITS sequencing, and the optimal operating conditions were explored by the simulated wastewater experiment. Build a complex flora with flocculant-producing bacteria preserved in the laboratory. Explore the effects of water temperature, pH, and initial ammonia–nitrogen concentration on the treatment of livestock wastewater with high ammonia–nitrogen by immobilized complex flora. The most similar of this strain is Phialemoniopsis curvata TWCC 58054 s, and the degradation rate of ammonia–nitrogen is 96.90% under the conditions of ammonia–nitrogen concentration of 50.00 mg/L, the water temperature of 30 ℃, pH = 7.2–7.4, and shaking culture for 7 days. At 400.00 mg/L, the water temperature of 30 ℃, pH = 8, shaking culture for 36 h, the degradation rate of ammonia–nitrogen was 57.39%. The optimal ratio of ammonia–nitrogen degrading bacteria to flocculant-producing bacteria is 1:3, and the ammonia–nitrogen degradation rate of immobilized complex bacteria is 85.43–89.84% after shaking culture for 36 h at the influent ammonia–nitrogen concentration of 400.00–500.00 mg/L, the water temperature of 30 ℃ and pH value of 8. The experimental results provided a new type of high-efficiency ammonia–nitrogen degrading bacteria, and described in detail the effects of the ratio of complex bacteria, pH, water temperature, influent concentration, on its treatment of livestock wastewater. It fills the gap of the new strain in ammonia–nitrogen degradation ability and immobilized complex flora.
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References
Yuan J. Research status of ammonia-nitrogen wastewater treatment methods [J]. Sci technol horiz, 2020, 160–161
Yang R, Zhang W, He R et al (2020) Summary of ammonia-nitrogen wastewater treatment technology [J]. Zhejiang Chem Ind 51:42–45
Tong W, Wang J, Liu D. Present situation and development of high concentration ammonia-nitrogen wastewater treatment [J]. Ind water treat, 2002, 9–12
Li H, Bi J (2020) Construction and effect of denitrifying complex flora [J]. Environ pollut prev 42:707–711 +716
Zhu X (2020) Bioaugmentation technology and its application in water pollution control [J]. Environ Dev 32(119):121
Wang Y, Cai W, Wang H (2020) Review of research on bioaugmentation technology and its application in sewage treatment [J]. J Ecol Environ 29:1062–1070
Ma H, He Q. Effective application of bioaugmentation technology in water pollution control [J]. Sci technol innov appl 2020 165–166
Guo Y. Application status and future trend of bioaugmentation technology in sewage treatment [J].Chem Manag 2020 54–55
Zhan Y, Wei Y, Tao X et al (2021) Screening, identification and application in aerobic composting of high-temperature oil-degrading strains [J]. Environ pollut prev 43:812–818
Wu Y, Ren X, Kou Y et al (2020) Strengthening effect of compound microbial inoculum on the treatment of high concentration ammonia-nitrogen wastewater [J]. Sci Technol Eng 20:10544–10549
Ding Q, Xie M, Chen F et al (2019) Isolation and identification of a strain with high efficiency of removing ammonia-nitrogen and nitrate nitrogen and its application in biological floc shrimp culture [J]. China Fish Sci 26:959–970
Motamedi H, Jafari M (2020) Screening heterotrophic ammonia removal and aerobic denitrifying bacteria from wastewater of ammonia production units of a petrochemical industry [J]. Curr Microbiol 77:2207–2214
Xu X. Preparation of embedded anaerobic ammonium oxidation bacteria and experimental study on treatment of low temperature and low ammonia-nitrogen wastewater [D]. Shenyang Jianzhu university 2020 p 104
Wang W, Ding Y, Wang Y et al (2016) Treatment of rich ammonia nitrogen wastewater with polyvinyl alcohol immobilized nitrifier biofortified constructed wetlands [J]. Ecol Eng 94:7–11
Wei D. Study on the treatment of ammonia and nitrite nitrogen in aquaculture water by immobilized compound bacteria [D]. Ocean University of China,2014 p 76
Yuan Z. Screening of high-efficient strains from domestic sewage in villages and towns and study on their degradation ability [D]. Anhui University of Engineering, 2011 p 72
Wang J, Wang L, Li H et al (2019) Screening and degradation characteristics of ammonia-nitrogen degrading bacteria in polluted water [J]. Environ Pollut Prev 41(772–776):782
Zhu P. Degradation of ammonia-nitrogen in urban landscape water by complex immobilized nitrifying bacteria [D]. Wuhan University 2019
Zhu P, Zhao W, Zhang Q (2019) Degradation of ammonia-nitrogen in small landscape water by complex immobilized nitrifying bacteria [D]. Water Treat Technol 45:18–22
Zhang C, Shang R, Wang J et al (2018) Screening, identification and degradation characteristics of an ammonia-nitrogen degrading bacterium [J]. Anhui Agric Sci 46:53–56
Chen H, Shine W, Kong W et al (2019) Screening and identification of ammonia-nitrogen degrading bacteria and study on water quality control effect of compound bacteria [J]. J Aquat Biology 43:875–883
Zhang B, Wang M, Qu J et al (2021) Characterization and mechanism analysis of tylosin biodegradation and simultaneous ammonia nitrogen removal with strain Klebsiella pneumoniae TN-1 [J]. Bioresource Technol 336:125342
Sun X, Shen Q, Wu Y et al (2020) Screening and preliminary application of ammonia-nitrogen degrading microorganisms [J]. Zhejiang Agric J 32:1683–1691
Cui L. Study on the construction of a compound microbial agent and its treatment of domestic sewage [D]. Northeastern University 2008 p 120
State Environmental Protection Administration. GB18596–2001, Discharge standards of pollutants for livestock and poultry breeding [S] 2003
Harrison JP, Hallsworth JE, Cockell CS (2015) Reduction of the temperature sensitivity of Halomonas hydrothermalis by iron starvation combined with microaerobic conditions [J]. Appl Environ Microb 81:2156–2162
Gu Y, Yu K, Ren Y et al (2021) Screening, identification and fermentation conditions optimization of a high concentration ammonia-nitrogen tolerant bacteria [J]. Food Ind Sci Technol 42:110–118
Zhang ZQ, Lin B, Xia SQ et al (2007) Construction of complex bacterial flora and kinetics of the produced microbial flocculants [J]. J Environ Eng 1(1):4
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This study was supported by Changzhou Science and Technology Support Program (CE20205002) and Jiangsu Postgraduate Research Innovation Program (KYCX20_2562, KYCX20_2605).
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Ruyi Li: Methodology, Visualization, Writing—original draft. Xian Xiao and Yuan Zhao: Writing—review & editing, Conceptualization, Visualization. Baohua Tu and Yimin Zhang: Writing—review & editing.
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Li, R., Xiao, X., Zhao, Y. et al. Screening of efficient ammonia–nitrogen degrading bacteria and its application in livestock wastewater. Biomass Conv. Bioref. 14, 8513–8521 (2024). https://doi.org/10.1007/s13399-022-03049-0
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DOI: https://doi.org/10.1007/s13399-022-03049-0