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Biomass colonization and bioconversion of the molecular characterized Oxalobacter formigenes to mitigate calcium oxlate urolithiasis

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Abstract

Calcium oxalate (CaOx) is one of the common causes of kidney stones and accounts for 40 to 50% of all uroliths in cats. Oxalobacter formigenes, an oxalate-degrading intestinal microbiota, has been hypothesized to play a protective role against CaOx urolithiasis due to its capability to degrade oxalate. This study was designed to reveal the association between biomass colonization of O. formigenes and clinical occurrence of CaOx urolithiasis in household tomcats. Fifteen tomcats were allocated into three groups (healthy control (n = 5), static chronic kidney disease (static CKD) (n = 4), and progressive CKD (n = 6)) based on diagnosis of CaOx urolithiasis and disease progression. Fecal samples were collected from all tomcats, genomic DNA was extracted, and oxc, a gene specific for O. formigenes, was quantified using real-time PCR. Additionally, the clinical association between blood serum urea, creatinine, and relative abundance of oxc gene among different groups was examined. The oxc gene was detected in all cats in various frequency; however, its relative abundance was significantly higher in progressive CKD group compared to static CKD and control groups. In summary, our results suggest a protective role of O. formigenes against calcium oxalate urolithiasis only in static CKD. Further studies are required in a larger group of cats to help illustrate the protective role of O. formigenes in the pathophysiology of calcium oxalate urolithiasis in cats.

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Acknowledgements

We gratefully acknowledge the clinic crew for animal handling during sampling and examination procedure.

Author information

Authors and Affiliations

  1. Department of Animal Medicine, College of Veterinary Medicine, Kafr-Elsheikh University, Kafr El-Shaikh, Egypt

    Mohamed Donia, Naglaa Gomaa, Midhat Nassif, Yamen Hegazy & Mohamed Abdelmegeid

  2. Department of Animal Medicine, College of Veterinary Medicine, Benha University, Banha, Egypt

    Mohamed Zeineldin

  3. Centro Universitario UAEM-Temascaltepec, Universidad Autónoma del Estado de México, Toluca, Mexico

    Jose Cedillo Monroy

  4. Unidad Académica de Ciencias Agropecuarias Y Ambientales, Universidad Autónoma de Guerrero, Iguala de La Independencia, Guerrero, Mexico

    Edson Brodeli Figueroa Pacheco

  5. Instituto de Ciencias Biomedicas, Departamento De Ciencias Quimico-Biologicas, Universidad Autónoma de Ciudad Juárez, Ciudad Juárez, Mexico

    Raymundo Rene Rivas-Caceres

  6. Higher Colleges of Technology, Abu Dhabi, UAE

    Rabiha Seboussi

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  1. Mohamed Donia
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Contributions

MD, MZ, NG, MN, YH, RS, and MA conceived and designed the experiment; MD, MA, and MZ conducted the experiment and data analysis; MD, MZ, NG, MN, YH, RS, JCM, RS, MA, and RR prepared the manuscript. All authors approved of the manuscript.

Corresponding author

Correspondence to Raymundo Rene Rivas-Caceres.

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Ethics approval

Animal studies have been approved by ethical committee. The research was performed in accordance with the ethical standard laid down in the 1996 declaration of Helsinki and its later amendments.

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The authors declare no competing interests.

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Highlights

Oxalobacter formigenes is a well-characterized oxalate metabolizing bacterium.

Oxalobacter formigenes play a vital role in reducing the exogenous urinary oxalates.

Oxalobacter formigenes has a protective role against CaOx urolithiasis in tomcats.

The oxc gene was detected in higher abundance in progressive CKD in tomcats.

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Donia, M., Zeineldin, M., Gomaa, N. et al. Biomass colonization and bioconversion of the molecular characterized Oxalobacter formigenes to mitigate calcium oxlate urolithiasis. Biomass Conv. Bioref. 14, 5197–5203 (2024). https://doi.org/10.1007/s13399-022-02704-w

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  • DOI: https://doi.org/10.1007/s13399-022-02704-w

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