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Exploring an alternative source of DIETer to mitigate ammonia inhibition of swine manure by inoculum treating brewery wastewater

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Abstract

Bioaugmentation of ammonia inhibition was hindered by the availability of DIETer inoculum. The unacclimated ethanol-fed inoculum treating brewery wastewater was compared with ammonia acclimated inoculum to explore a way of alternative DIETer source. The overall performance, evolution of microbial community and metabolic functions were investigated. The unacclimated inoculum (194.71 ± 1.98 mL CH4/VSadded) demonstrated feasible recovery in methane yield under ammonia stress (4000 mg/L) compared with acclimated inoculum (191.10 ± 1.46 mL CH4/VSadded). The Methanothrix in the unacclimated group significantly (p < 0.05) decreased 31.60% than the acclimated group. Functional analysis indicated that the unacclimated Methanothrix-dominated inoculum had higher relative abundances in key enzymes of hydrogenotrophic methanogenesis. These statistical results revealed that the inoculum treating brewery wastewater serves as a firm foundation for the alternative DIETer inoculum source, broadening inoculums for mitigating ammonia stress. The threshold-type inhibition function also inspired a strategy to replace ethanol with ammonia (3000 ~ 8000 mg/L) for promoting DIETer, which could maintain direct interspecies electron transfer as the predominant working mode of interspecies electron exchange.

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Acknowledgements

This work was supported by the Major Science and Technology Program for Water Pollution Control and Treatment of China (2017ZX07102-001). The authors would like to thank Daan Van Hauwermeiren from Ghent University for the global optimism of the inhibition function.

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Authors and Affiliations

  1. State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China

    Dawei Yu, Buhe Chulu & Yuansong Wei

  2. Department of Water Pollution Control Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

    Dawei Yu, Buhe Chulu & Yuansong Wei

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Dawei Yu, Buhe Chulu & Yuansong Wei

  4. Department of Mathematical Modelling, Statistics and Bioinformatics, BIOMATH, Ghent University, B-9000, Gent, Belgium

    Min Yang

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  1. Dawei Yu
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Correspondence to Yuansong Wei.

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Yu, D., Chulu, B., Yang, M. et al. Exploring an alternative source of DIETer to mitigate ammonia inhibition of swine manure by inoculum treating brewery wastewater. Biomass Conv. Bioref. 13, 11905–11916 (2023). https://doi.org/10.1007/s13399-021-02119-z

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  • DOI: https://doi.org/10.1007/s13399-021-02119-z

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