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Biorefinery potential of newly isolated yeast Clavispora lusitaniae for co-production of erythritol and ethanol

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Abstract

This study reports the biorefinery potential of a newly isolated yeast, Clavispora lusitaniae JARR-1, for production of erythritol as a sweet low-calorie food additive, along with co-production of ethanol as a biofuel candidate. The co-production process for synthesis of ethanol and erythritol was optimized using two-stage response surface methodology employing Placket-Burman (PB) and Central Composite Rotatable (CCR) designs. PB design indicated that the medium components, glucose, peptone, urea, and MgSO4, significantly affected (p < 0.05) erythritol production. Furthermore, optimization of erythritol and ethanol co-production process by CCR design revealed the significant (p < 0.05) interactive effects of glucose and peptone on erythritol production and glucose, peptone, and MgSO4 on ethanol production. Under optimized co-production process, maximum titers of erythritol and ethanol were 22.02 ± 0.04 g/L and 68.00 ± 0.02 g/L, respectively. The optimized process resulted in fivefold increase in erythritol production. Thus, C. lusitaniae JARR-1 has potential for sustainable co-production of erythritol and ethanol using biorefinery approach.

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Highlights

• Erythritol production by Clavispora lusitaniae JARR-1

• Erythritol and ethanol co-production optimization by RSM

• fivefold erythritol production (from 4.2 ± 0.19 to 22.02 ± 0.04 g/L) after optimization

• Glucose (20% wt.) & peptone (3% wt.) significantly affected erythritol production

• Maximum ethanol co-production of 68.00 ± 0.02 g/L

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Acknowledgements

The authors thank the University Grants Commission (UGC), Govt. of India, for providing financial support under Dr. D.S. Kothari post-doctoral fellowship scheme and the Central University of Haryana, Mahendergarh, for infrastructural support. JKS acknowledges financial support from SERB-DST (grant no. ECR/2016/000929/LS). H acknowledges CSIR-UGC for her NET-junior research fellowship. Authors also acknowledge Dr. Rishi Gupta for providing his inputs in designing the study.

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Authors and Affiliations

  1. Department of Microbiology, Central University of Haryana, Mahendergarh, 123031, Haryana, India

    Rishikesh Shukla,  Hemansi & Jitendra Kumar Saini

  2. Department of Biotechnology, Atmiya University, Yogidham Gururkul, Kalawad Road, Rajkot, 360005, Gujarat, India

    Rishikesh Shukla

  3. Department of Microbiology, University of Delhi South Campus, New Delhi, 120001, India

    Gaurav Kumar & Ramesh Chander Kuhad

  4. Department of Microbiology, Shree M & N Virani Science College (Autonomous), Atmiya University, Yogidham Gurukul, Kalawad Road, Rajkot, 360005, Gujarat, India

    Shraddha Shukla

  5. Central University of Haryana, Mahendergarh, 123031, Haryana, India

    Ramesh Chander Kuhad

  6. SGT University, Gurugram-Badli Road, Gurugram, Pin-122505, Haryana, India

    Ramesh Chander Kuhad

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  1. Rishikesh Shukla
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Shukla, R., Hemansi, Kumar, G. et al. Biorefinery potential of newly isolated yeast Clavispora lusitaniae for co-production of erythritol and ethanol. Biomass Conv. Bioref. 13, 8061–8073 (2023). https://doi.org/10.1007/s13399-021-02073-w

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