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Microwave-assisted pretreatment of harmful algal blooms for microbial oil-centered biorefinery approach

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Abstract

Utilization of harmful algal blooms (HABs) for the cultivation of oleaginous microorganisms can provide dual benefits of mitigating the toxicity from the aquatic reservoirs and generation of copious media for biodiesel production. In the present investigation, microwave-assisted dilute alkali-freeze pretreatment was optimized to develop a low-cost growth medium from HAB dried biomass. The electron micrographs along with the elemental analysis confirmed the efficient breakage of HABs after the microwave-assisted hydrolysis treatment as compared with the acid hydrolysis. Moreover, the sugar analysis revealed ~ 46% higher carbohydrate content in microwave-assisted hydrolysate as compared with acid hydrolysate. The microwave-assisted hydrolysate and conventional dilute acid hydrolysate were then used to cultivate microalga (Chlorella minutissima) and yeast (Trichosporon cutaneum) for biomass and lipid accumulation and compared to artificial media. Microalga showed ~ 1.3- and 2-fold higher dry cell weight (DCW) and lipid content, respectively, while the yeast growth increased by ~ 27% with lipid content of 30%. The fatty acid profiles and biodiesel properties were also amenable to the international biodiesel standards. Hence, the present study provides a proof-of-concept of utilizing HAB hydrolysate for culturing oleaginous microorganisms for potential biodiesel production.

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Funding

This research work was carried out by the grant provided by DST, Govt. of India under the project (Indo Russian -INT/RUS/RFBR/347). MSV acknowledge the receipt of grant for research work from Russian Foundation for Basic Research (Indo-Russian project No. 18-58-45009).

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Authors and Affiliations

  1. Algal Research and Bioenergy Lab, Department of Chemistry, Uttaranchal University, Dehradun, Uttarakhand, 248007, India

    Vinod Kumar, Neha Arora, Shivam Pandey & Krishna Kumar Jaiswal

  2. Department of Biotechnology, Dolphin (P.G.) Institute of Biomedical and Natural Sciences, Dehradun, Uttarakhand, 248001, India

    Manisha Nanda

  3. Joint Institute for High Temperatures of the Russian Academy of Sciences, 13/2 Izhorskaya St, Moscow, 125412, Russia

    M. S. Vlaskin

  4. Faculty of Applied Sciences and Biotechnology, Shoolini University, Solan, HP, India

    P. K. Chauhan

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  1. Vinod Kumar
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  2. Neha Arora
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  3. Shivam Pandey
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  4. Krishna Kumar Jaiswal
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  5. Manisha Nanda
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  6. M. S. Vlaskin
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Correspondence to Vinod Kumar or Neha Arora.

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Kumar, V., Arora, N., Pandey, S. et al. Microwave-assisted pretreatment of harmful algal blooms for microbial oil-centered biorefinery approach. Biomass Conv. Bioref. 12, 3097–3105 (2022). https://doi.org/10.1007/s13399-020-00941-5

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