Skip to main content
SpringerLink
Log in

Antioxidant and antibacterial potential of crude extract of soil fungus Periconia sp. (SSS-8)

  • Research Article-Biological Sciences
  • Published:
Arabian Journal for Science and Engineering Aims and scope Submit manuscript

Abstract

The present study aimed at screening nine soil fungi isolated from Puttaparthi, India for their antioxidant and antibacterial activities. Isolate (SSS-8), with notable antioxidant and antibacterial activities was identified as Periconia sp. using ITS gene sequence and morphology. The crude culture extract of SSS-8 obtained by growing the fungus in Potato Dextrose Broth (PDB) for 14 days at 30 °C and 120 rpm, exhibited DPPH free radical scavenging potential of about 89.62% at 1 mg/mL concentration. SSS-8 also showed promising antibacterial activity against bacteria Staphylococcus aureus, Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA) and moderate activity against Enterococcus faecalis using disk diffusion method at 25 mg/mL concentration. Chemical profiling of the crude culture extract of SSS-8 was carried out using gas chromatography-mass spectrometry (GC–MS). Some of the major compounds identified such as 2,4-di-tert-butylphenol, 1-tetracosanol, 2-phenylethanol, p-menthan-3-one are known to be antibacterial compounds and compounds such as methyl 3,4-dihydroxybenzoate, 1-octadecene, 2,4-di-tert-butylphenol, 1-tetracosanol are potential antioxidative agents. In conclusion, soil fungus Periconia sp. (SSS-8) could be explored as an effective producer of antioxidant and antibacterial compounds for utility in pharmaceutical and nutraceutical applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Xu, L.L.; Cao, F.; Tian, S.S.; Zhu, H.J.: Alkaloids and polyketides from the soil fungus Aspergillus terreus and their antibacterial activities. Chem. Nat. Compd. 53, 1212–1215 (2017). https://doi.org/10.1007/s10600-017-2243-5

    Article  Google Scholar 

  2. Bérdy, J.: Bioactive microbial metabolites. J. Antibiot. (Tokyo) 58, 1–26 (2005). https://doi.org/10.1038/ja.2005.1

    Article  Google Scholar 

  3. Sharma, D.; Pramanik, A.; Kumar, P.: Evaluation of bioactive secondary metabolites from endophytic fungus Pestalotiopsis neglecta BAB-5510 isolated from leaves of Cupressus torulosa D. Don. Biotech 6, 1–14 (2016). https://doi.org/10.1007/s13205-016-0518-3

    Article  Google Scholar 

  4. Feofilova, E.P.: The kingdom fungi : heterogeneity of physiological and biochemical properties and relationships with plants, animals and prokaryotes. Review 37, 124–137 (2001)

    Google Scholar 

  5. Mohamed, H.F.: Molecular analysis and anticancer properties of two identified isolates, Fusarium solani and Emericella nidulans isolated from Wady El–Natron soil in Egypt against Caco–2 (ATCC) cell line. Asian Pac J Trop Biomed 2, 863–869 (2012). https://doi.org/10.1016/S2221-1691(12)60244-5

    Article  Google Scholar 

  6. Abdel-Aziz, M.S.; Ghareeb, M.A.; Saad, A.M.; Refahy, L.A.; Hamed, A.A.: Chromatographic isolation and structural elucidation of secondary metabolites from the soil-inhabiting fungus Aspergillus fumigatus 3T-EGY. Acta Chromatogr 30, 243–249 (2018). https://doi.org/10.1556/1326.2017.00329

    Article  Google Scholar 

  7. Strobel, G.A.: Endophytes as sources of bioactive products. Microbes Infect 5, 535–544 (2003). https://doi.org/10.1016/S1286-4579(03)00073-X

    Article  Google Scholar 

  8. Pham-Huy, L.A.; He, H.; Pham-Huy, P.C.: Free radicals, antioxidants in disease and health. Int J Biomed Sci 4, 89–96 (2008)

    Google Scholar 

  9. Behera, B.C.; Verma, N.; Sonone, A.; Makhija, U.: Determination of antioxidative potential of lichen Usnea ghattensis in vitro. LWT Food Sci Technol 39, 80–85 (2006). https://doi.org/10.1016/j.lwt.2004.11.007

    Article  Google Scholar 

  10. Patil, M.P.; Patil, R.H.; Maheshwari, V.L.: Biological activities and identification of bioactive metabolite from endophytic Aspergillus flavus L7 Isolated from Aegle marmelos. Curr Microbiol 71, 39–48 (2015). https://doi.org/10.1007/s00284-015-0805-y

    Article  Google Scholar 

  11. Gunasekaran, R.; Janakiraman, D.; Rajapandian, S.G.K.; Appavu, S.P.; Namperumalsamy Venkatesh, P.; Prajna, L.: Periconia species - an unusual fungal pathogen causing mycotic keratitis. Indian J Med Microbiol 39, 36–40 (2021). https://doi.org/10.1016/j.ijmmb.2020.10.006

    Article  Google Scholar 

  12. Markovskaja, S.; Kačergius, A.: Morphological and molecular characterisation of Periconia pseudobyssoides sp. nov. and closely related P. byssoides. Mycol Prog 13, 291–302 (2014). https://doi.org/10.1007/s11557-013-0914-6

    Article  Google Scholar 

  13. Zhang, D.; Tao, X.; Chen, R.; Liu, J.; Li, L.; Fang, X.; Yu, L.; Dai, J.: Pericoannosin A, a polyketide synthase-nonribosomal peptide synthetase hybrid metabolite with new carbon skeleton from the endophytic fungus periconia sp. Org Lett 17, 4304–4307 (2015). https://doi.org/10.1021/acs.orglett.5b02123

    Article  Google Scholar 

  14. Teles, H.L.; Sordi, R.; Silva, G.H.; Castro-Gamboa, I.; da Silva Bolzani, V.; Pfenning, L.H.; de Abreu, L.M.; Costa-Neto, C.M.; Young, M.C.; Araújo, Â.R.: Aromatic compounds produced by Periconia atropurpurea, an endophytic fungus associated with Xylopia aromatica. Phytochemistry 67, 2686–2690 (2006). https://doi.org/10.1016/j.phytochem.2006.09.005

    Article  Google Scholar 

  15. Shin DS, Oh MN, Yang HC, Oh KB (2005) Biological characterization of periconicins, bioactive secondary metabolites, produced by Periconia sp. OBW 15

  16. Subba Rao, N.; John Devadas, D.: Fluoride incidence in groundwater in an area of Peninsular India. Environ Geol 45, 243–251 (2003). https://doi.org/10.1007/s00254-003-0873-3

    Article  Google Scholar 

  17. Waksman, S.A.: A method for counting the number of fungi in the soil. J Bacteriol 7, 339–33941 (1922)

    Article  Google Scholar 

  18. Arora, D.S.; Chandra, P.: Antioxidant activity of Aspergillus fumigatus. ISRN Pharmacol 2011, 1–11 (2011). https://doi.org/10.5402/2011/619395

    Article  Google Scholar 

  19. Brand-Williams, W.; Cuvelier, M.E.; Berset, C.: Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28, 25–30 (1995). https://doi.org/10.1016/S0023-6438(95)80008-5

    Article  Google Scholar 

  20. Kumar, R.; Sukhvinder, S.; Purewal, S.: Phenolic content, antioxidant potential and DNA damage protection of pearl millet (Pennisetum glaucum) cultivars of North Indian region. J Food Meas Charact 11, 126–133 (2017). https://doi.org/10.1007/s11694-016-9379-z

    Article  Google Scholar 

  21. Balouiri, M.; Sadiki, M.; Ibnsouda, S.K.: Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 6, 71–79 (2016). https://doi.org/10.1016/j.jpha.2015.11.005

    Article  Google Scholar 

  22. Monggoot, S.; Pichaitam, T.; Tanapichatsakul, C.; Pripdeevech, P.: Antibacterial potential of secondary metabolites produced by Aspergillus sp., an endophyte of Mitrephora wangii. Arch Microbiol 200, 951–959 (2018). https://doi.org/10.1007/s00203-018-1511-5

    Article  Google Scholar 

  23. Zhang, Y.J.; Zhang, S.; Liu, X.Z.; Wen, H.A.; Wang, M.: A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Lett Appl Microbiol (2010). https://doi.org/10.1111/j.1472-765X.2010.02867.x

    Article  Google Scholar 

  24. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols. pp. 315–322. Elsevier

  25. Cantrell, S.A.; Hanlin, R.T.; Emiliano, A.: Periconia variicolor sp. nov., a new species from Puerto Rico. Mycologia 99, 482–487 (2007). https://doi.org/10.1080/15572536.2007.11832573

    Article  Google Scholar 

  26. Arora, D.S.; Chandra, P.: In vitro antioxidant potential of some soil fungi: screening of functional compoundsand their purification from penicillium citrinum. Appl Biochem Biotechnol 165, 639–651 (2011). https://doi.org/10.1007/s12010-011-9282-3

    Article  Google Scholar 

  27. Gao, W.; Chai, C.; He, Y.; Li, F.; Hao, X.; Cao, F.; Gu, L.; Liu, J.; Hu, Z.; Zhang, Y.: Periconiastone a, an antibacterial ergosterol with a pentacyclo heptadecane system from Periconia sp. TJ403-rc01. Org Lett 21, 8469–8472 (2019). https://doi.org/10.1021/acs.orglett.9b03270

    Article  Google Scholar 

  28. Kim, S.; Shin, D.S.; Lee, T.; Oh, K.B.: Periconicins, two new fusicoccane diterpenes produced by an endophytic fungus Periconia sp. with antibacterial activity. J Nat Prod 67, 448–450 (2004). https://doi.org/10.1021/np030384h

    Article  Google Scholar 

  29. Zhu, Y.-J.; Zhou, H.-T.; Hu, Y.-H.; Tang, J.-Y.; Su, M.-X.; Guo, Y.-J.; Chen, Q.-X.; Liu, B.: Antityrosinase and antimicrobial activities of 2-phenylethanol, 2-phenylacetaldehyde and 2-phenylacetic acid. Food Chem 124, 298–302 (2011). https://doi.org/10.1016/j.foodchem.2010.06.036

    Article  Google Scholar 

  30. Haiyan, G.; Lijuan, H.; Shaoyu, L.; Chen, Z.; Ashraf, M.A.: Antimicrobial, antibiofilm and antitumor activities of essential oil of Agastache rugosa from Xinjiang, China. Saudi J Biol Sci 23, 524–530 (2016). https://doi.org/10.1016/j.sjbs.2016.02.020

    Article  Google Scholar 

  31. Aissaoui, N.; Mahjoubi, M.; Nas, F.; Mghirbi, O.; Arab, M.; Souissi, Y.; Hoceini, A.; Masmoudi, A.S.; Mosbah, A.; Cherif, A.; Klouche-Khelil, N.: Antibacterial potential of 2,4-Di-tert-butylphenol and calixarene-based prodrugs from thermophilic Bacillus licheniformis isolated in Algerian hot spring. Geomicrobiol J 36, 53–62 (2019). https://doi.org/10.1080/01490451.2018.1503377

    Article  Google Scholar 

  32. Tsuda, T.; Watanabe, M.; Ohshima, K.; Yamamoto, A.; Kawakishi, S.; Osawa, T.: Antioxidative components isolated from the seed of tamarind (Tamarindus indica L.). J Agric Food Chem 42, 2671–2674 (1994). https://doi.org/10.1021/jf00048a004

    Article  Google Scholar 

  33. Yoon, M.; Jeong, T.; Park, D.; Ming-zhe, X.; Hyun-Woo, O.; Kyoung-Bin, S.; Woo Song, L.; Ho-Yong, P.: Antioxidant effects of quinoline alkaloids and 2,4-Di-tert-butylphenol isolated from Scolopendra subspinipes. Biol Pharm Bull 29, 735–739 (2006)

    Article  Google Scholar 

  34. Tonisi, S.; Okaiyeto, K.; Hoppe, H.; Mabinya, L.V.; Nwodo, U.U.: Chemical constituents, antioxidant and cytotoxicity properties of Leonotis leonurus used in the folklore management of neurological disorders in the Eastern Cape, South Africa. 3 Biotech 10, 1–14 (2020). https://doi.org/10.1007/s13205-020-2126-5

    Article  Google Scholar 

  35. Faridha Begum, I.; Mohankumar, R.; Jeevan, M.; Ramani, K.: GC–MS analysis of bio-active molecules derived from Paracoccus pantotrophus FMR19 and the antimicrobial activity against bacterial pathogens and MDROs. Indian J. Microbiol. 56, 426–432 (2016). https://doi.org/10.1007/s12088-016-0609-1

    Article  Google Scholar 

Download references

Acknowledgements

The authors dedicate this work to Bhagawan Sri Sathya Sai Baba, Founder Chancellor of the Sri Sathya Sai Institute of Higher Learning. The authors acknowledge UGC-SAP (DRS) and DST-FIST, Government of India for the infrastructural support to the Department of Biosciences, SSSIHL, Prasanthi Nilayam. Skanda S is thankful to UGC, New Delhi, for the award of UGC-BSR fellowship. The authors thank Dr Pradeep BE, Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, for providing the bacterial cultures used in the study.

Author information

Authors and Affiliations

  1. Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam Campus, Puttaparthi, Andhra Pradesh, 515134, India

    S. Skanda & B. S. Vijayakumar

Authors
  1. S. Skanda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. S. Vijayakumar
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

SS carried out the laboratory work and drafted the manuscript; VBS supervised the study design and reviewed the manuscript. The authors approved the submission of the final version of the manuscript for publication.

Corresponding author

Correspondence to S. Skanda.

Ethics declarations

Conflict of interest

Skanda S and Vijayakumar B S declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Skanda, S., Vijayakumar, B.S. Antioxidant and antibacterial potential of crude extract of soil fungus Periconia sp. (SSS-8). Arab J Sci Eng 47, 6707–6714 (2022). https://doi.org/10.1007/s13369-021-06061-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13369-021-06061-0

Keywords

Search

Navigation