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Antioxidant and Anti-inflammatory Metabolites of a Soil-Derived Fungus Aspergillus arcoverdensis SSSIHL-01

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Abstract

Soil houses a vast array of microbial diversity. Cultured soil microbes have been a good source of many commercial drugs. In the present study, a fungal culture (SSSIHL-01) isolated from soil has been identified as Aspergillus arcoverdensis through morphology and ITS gene sequence. Extracellular culture extract and mycelial extract of the strain SSSIHL-01 were obtained using specific conditions and were evaluated for antioxidant and anti-inflammatory activities. Culture extract at 700 µg/mL concentration, showed strong DPPH free radical scavenging capacity with 95.06% comparable with the standard ascorbic acid. At 1 mg/mL concentration, mycelial extract inhibited heat induced Bovine Serum Albumin denaturation of about 31.54% compared to that of 51% produced by the standard diclofenac sodium. Chemical profiling of both the culture and mycelial extracts were investigated using gas chromatography-mass spectrometry. Some of the major compounds identified from the culture extract were 2,4-ditert-butylphenol, 1-heptacosanol, 1-octadecene, 1-nonadecene that are known to be antioxidative. Mycelial extract presented some major compounds such as ethyl linoleate, oleic acid, n-hexadecanoic acid and ethyl palmitate that are reported to exhibit anti-inflammatory activity. Thus, our study highlights the significance of Aspergillus arcoverdensis as an effective producer of antioxidant and anti-inflammatory compounds for future utility in pharmaceutical and cosmeceutical applications.

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Acknowledgements

The authors are grateful to Bhagawan Sri Sathya Sai Baba for constant motivation and support. The authors acknowledge UGC-SAP (DRS), DST-FIST and DBT-BIF, Government of India for the infrastructural support to the Department of Biosciences, SSSIHL, Prasanthi Nilayam. Skanda S is thankful to UGC, New Delhi, for the award of UGC-BSR fellowship.

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  1. Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Puttaparthi, Andhra Pradesh, India

    S. Skanda & B. S. Vijayakumar

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Correspondence to S. Skanda.

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Skanda, S., Vijayakumar, B.S. Antioxidant and Anti-inflammatory Metabolites of a Soil-Derived Fungus Aspergillus arcoverdensis SSSIHL-01. Curr Microbiol 78, 1317–1323 (2021). https://doi.org/10.1007/s00284-021-02401-3

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