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Characterization of a DAPI-RIT-DAPI System for Gas-Phase Ion/Molecule and Ion/Ion Reactions

  • Research Article
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Journal of The American Society for Mass Spectrometry

Abstract

The discontinuous atmospheric pressure interface (DAPI) has been developed as a facile means for efficiently introducing ions generated at atmospheric pressure to an ion trap in vacuum [e.g., a rectilinear ion trap (RIT)] for mass analysis. Introduction of multiple beams of ions or neutral species through two DAPIs into a single RIT has been previously demonstrated. In this study, a home-built instrument with a DAPI-RIT-DAPI configuration has been characterized for the study of gas-phase ion/molecule and ion/ion reactions. The reaction species, including ions or neutrals, can be introduced from both ends of the RIT through the two DAPIs without complicated ion optics or differential pumping stages. The primary reactant ions were isolated prior to reaction and the product ions were mass analyzed after controlled reaction time period. Ion/molecule reactions involving peptide radical ions and proton-transfer ion/ion reactions have been carried out using this instrument. The gas dynamic effect due to the DAPI operation on internal energy deposition and the reactivity of peptide radical ions has been characterized. The DAPI-RIT-DAPI system also has a unique feature for allowing the ion reactions to be carried out at significantly elevated pressures (in 10–1 Torr range), which has been found to be helpful to speed up the reactions. The viability and flexibility of the DAPI-RIT-DAPI system for the study of gas-phase ion reactions have been demonstrated.

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Acknowledgment

This research was supported by the National Science Foundation to Z.O. (Project 0847205-CHE) and to Y.X. (CHE-1308114).

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Authors and Affiliations

  1. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA

    Ziqing Lin, Sandilya Garimella, Linfan Li, Tsung-Chi Chen, Wei Xu & Zheng Ouyang

  2. Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA

    Lei Tan & Yu Xia

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  1. Ziqing Lin
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  2. Lei Tan
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Correspondence to Yu Xia or Zheng Ouyang.

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Lin, Z., Tan, L., Garimella, S. et al. Characterization of a DAPI-RIT-DAPI System for Gas-Phase Ion/Molecule and Ion/Ion Reactions. J. Am. Soc. Mass Spectrom. 25, 48–56 (2014). https://doi.org/10.1007/s13361-013-0757-z

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  • DOI: https://doi.org/10.1007/s13361-013-0757-z

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