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Vacuum Ultraviolet Action Spectroscopy of Polysaccharides

  • Research Article
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Journal of The American Society for Mass Spectrometry

Abstract

We studied the optical properties of gas-phase polysaccharides (maltose, maltotetraose, and maltohexaose) ions by action spectroscopy using the coupling between a quadrupole ion trap and a vacuum ultraviolet (VUV) beamline at the SOLEIL synchrotron radiation facility (France) in the 7 to 18 eV range. The spectra provide unique benchmarks for evaluation of theoretical data on electronic transitions of model carbohydrates in the VUV range. The effects of the nature of the charge held by polysaccharide ions on the relaxation processes were also explored. Finally the effect of isomerization of polysaccharides (with melezitose and raffinose) on their photofragmentation with VUV photons is presented.

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Acknowledgments

The authors thank the Pôle Scientifique de Modélisation Numérique (PSMN) at Lyon, France, for generous computational facilities. SOLEIL support is acknowledged under project #20110002. This work was supported by the Agence Nationale de la Recherche Scientifique, France, under the project ANR-08-BLAN-0065. The authors also thank the general technical staff of SOLEIL for running the facility. They thank J.-F. Gil for his help in the alignment of the ion trap with respect to the beamline. They thank Stéphane Chambert, Sébastien Redon, and Francis Canon for helpful discussion.

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Authors and Affiliations

  1. Université Lyon 1-CNRS, Université de Lyon, 69622, Villeurbanne cedex, France

    Quentin Enjalbert, Claire Brunet, Arnaud Vernier, Abdul-Rahman Allouche, Rodolphe Antoine, Philippe Dugourd & Jérôme Lemoine

  2. UMR5306, Institut Lumière Matière, Villeurbanne, France

    Quentin Enjalbert, Claire Brunet, Arnaud Vernier, Abdul-Rahman Allouche, Rodolphe Antoine & Philippe Dugourd

  3. UMR5180, Sciences Analytiques, Villeurbanne, France

    Quentin Enjalbert, Arnaud Vernier & Jérôme Lemoine

  4. R&D Proteomique, bioMérieux SA, 69280, Marcy l’Etoile, France

    Arnaud Vernier

  5. SOLEIL, l’Orme des Merisiers, St Aubin, BP48, , 91192, Gif sur Yvette Cedex, France

    Alexandre Giuliani & Laurent Nahon

  6. UAR1008, Cepia, Institut National de la Recherche Agronomique (INRA), BP 71627, 44316, Nantes Cedex 3, France

    Alexandre Giuliani

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  1. Quentin Enjalbert
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  2. Claire Brunet
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  4. Abdul-Rahman Allouche
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Correspondence to Rodolphe Antoine.

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Enjalbert, Q., Brunet, C., Vernier, A. et al. Vacuum Ultraviolet Action Spectroscopy of Polysaccharides. J. Am. Soc. Mass Spectrom. 24, 1271–1279 (2013). https://doi.org/10.1007/s13361-013-0657-2

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  • DOI: https://doi.org/10.1007/s13361-013-0657-2

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