Abstract
The smaller tea tortrix, Adoxophyes honmai Yasuda (Lepidoptera: Tortricidae), is one of the most common pests that affect the tea plant, Camellia sinensis (L.) O. Kuntze. Diacylhydrazine-analog insect growth regulators, such as tebufenozide, have been used as controls; however, the species began showing resistance to this compound in approximately 2004. Previously, we identified an amino acid mutation (A415V) in the ecdysone receptor gene (EcR) as a principal factor in tebufenozide resistance and developed a molecular diagnostic method to detect the EcR A415V mutation through polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP). However, for the continuous management of tebufenozide resistance in A. honmai, a simple and quick genetic diagnostic method that does not require specialized equipment is preferable. In this study, we developed a novel technique to detect the mutation using allele-specific loop-mediated isothermal amplification (AS-LAMP). This method enables the monitoring and detection of tebufenozide-resistance alleles much faster than PCR–RFLP and with moderate accuracy. Therefore, it could be a powerful tool for the early detection of tebufenozide-resistant A. honmai in tea plantations.
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26 October 2021
A Correction to this paper has been published: https://doi.org/10.1007/s13355-021-00763-5
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Acknowledgements
We are grateful to Ms. Akemi Inoue of NARO for assistance with DNA extraction. This research was supported by research grants from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics-based Technology for Agricultural Improvement; PRM-02), and the NARO Gender Equality Program.
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Uchibori-Asano, M., Uchiyama, T., Jouraku, A. et al. Development of allele-specific loop-mediated isothermal amplification (AS-LAMP) to detect the tebufenozide-resistant allele in the smaller tea tortrix, Adoxophyes honmai (Lepidoptera: Tortricidae). Appl Entomol Zool 57, 93–99 (2022). https://doi.org/10.1007/s13355-021-00756-4
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DOI: https://doi.org/10.1007/s13355-021-00756-4