Abstract
Aphis gossypii Glover is a cosmopolitan and polyphagous aphid species. When neonicotinoid-resistant clones of A. gossypii were recently found in Japan, it was subsequently discovered that a point mutation (R81T) in the D-loop region of the β1 subunit of the nicotinic acetylcholine receptor gene had conferred this resistance. To quickly identify this neonicotinoid-resistant allele, we developed a molecular diagnostics test using a multiplex polymerase chain reaction method that targeted the R81T mutation.
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References
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Acknowledgments
We are grateful to Mrs S. Okazaki (Oita Prefectural Agriculture, Forestry and Fisheries Research Center), T. Okamoto (Wakayama Research Center of Agriculture, Forestry and Fisheries) and S. Kubota (Ehime Research Institute of Agriculture, Forestry and Fisheries) for providing aphid samples. We also thank Ms N. Inose for helping with the molecular analysis. This work was supported by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics-based Technology for Agricultural Improvement; PRM-03).
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Toda, S., Hirata, K., Yamamoto, A. et al. Molecular diagnostics of the R81T mutation on the D-loop region of the β1 subunit of the nicotinic acetylcholine receptor gene conferring resistance to neonicotinoids in the cotton aphid, Aphis gossypii (Hemiptera: Aphididae). Appl Entomol Zool 52, 147–151 (2017). https://doi.org/10.1007/s13355-016-0449-9
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DOI: https://doi.org/10.1007/s13355-016-0449-9