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Genetic analysis of novel resistance sources and genome-wide association mapping identified novel QTLs for resistance to Zymoseptoria tritici, the causal agent of septoria tritici blotch in wheat

  • Plant Genetics • Original Paper
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Abstract

Septoria tritici blotch (STB) caused by Zymoseptoria tritici is one of the most important foliar diseases of wheat causing significant yield losses worldwide. In this study, a panel of bread wheat genotypes comprised 185 globally diverse genotypes were tested against 10 Z. tritici isolates at the seedling stage. Genome-wide association study (GWAS) using high-throughput DArTseq markers was performed and further gene expression analysis of significant markers trait association (MTAs) associated with resistance to STB was analyzed. Disease severity level showed significant differences among wheat genotypes for resistance to different Z. tritici isolates. We found novel landrace genotypes that showed highly resistance spectra to all tested isolates. GWAS analysis resulted in 19 quantitative trait loci (QTLs) for resistance to STB that were located on 14 chromosomes. Overall, 14 QTLs were overlapped with previously known QTLs or resistance genes, as well as five potentially novel QTLs on chromosomes 1A, 4A, 5B, 5D, and 6D. Identified novel resistance sources and also novel QTLs for resistance to different Z. tritici isolates can be used for gene pyramiding and development of durable resistance cultivars in future wheat breeding programs.

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Data availability

The datasets analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors acknowledge the Iranian Seed and Plant Improvement Institute (SPII) for their kind support in germplasm preparation and disease phenotyping experiments.

Funding

Financial support for genotypic analysis was received from Islamic Azad University, Sanandaj Branch, Iran.

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Authors and Affiliations

  1. Department of Agronomy and Plant Breeding, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

    Mozghan Mahboubi & Reza Talebi

  2. Keygene N.V, P.O. Box 216, 6700 AE, Wageningen, Netherlands

    Reza Talebi & Rahim Mehrabi

  3. Department of Biotechnology, College of Agriculture, Isfahan University of Technology, POBox 8415683111, Isfahan, Iran

    Rahim Mehrabi

  4. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahed University, Tehran, Iran

    Amir Mohammad Naji

  5. Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy

    Marco Maccaferri

  6. Laboratory of Phytopathology, Wageningen University and Research, Wageningen, The Netherlands

    Gert H. J. Kema

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  1. Mozghan Mahboubi
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  2. Reza Talebi
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  3. Rahim Mehrabi
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  4. Amir Mohammad Naji
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  5. Marco Maccaferri
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  6. Gert H. J. Kema
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Contributions

MM disease phenotyping, writing, data analysis; RT and RM conceptualization, methodology, resources, writing—review and editing, supervision, funding acquisition; AMJ methodology, software, data analysis; MM and GK review and editing.

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Correspondence to Reza Talebi or Rahim Mehrabi.

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Mahboubi, M., Talebi, R., Mehrabi, R. et al. Genetic analysis of novel resistance sources and genome-wide association mapping identified novel QTLs for resistance to Zymoseptoria tritici, the causal agent of septoria tritici blotch in wheat. J Appl Genetics 63, 429–445 (2022). https://doi.org/10.1007/s13353-022-00696-x

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