Abstract
Chacoan peccary (Catagonus wagneri, 2n=20) is the most endangered of three extant species of Tayassuidae. Its karyotype has been studied only by differential chromosome staining methods so far. To establish a comparative cytogenetic map of the peccary, we used cross-species hybridization with porcine (Sus scrofa, 2n=38) painting probes. Painting revealed 30 evolutionary conserved autosomal segments between pig and peccary. The q-arm of the submetacentric chromosome X is homologous to the porcine X chromosome, while the p-arm is composed of heterochromatin. Nucleolar organizer regions were detected on chromosomes 8 and 9 which are homologous to pig chromosomes 8 and 4/18, respectively. Fusions of chromosomes homologous to pig chromosomes 4/7 and 4/18 and fission of chromosome 7 are synapomorphic characters shared by Catagonus wagneri and Tayassu pecari but not by Pecari tajacu. Our results confirmed a high rate of karyotype evolution in Tayassuidae and a closer relationship of Catagonus wagneri with Tayassu pecari than with Pecari tajacu.
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The authors are grateful to Jiri Vodicka, veterinarian in Prague Zoo, for long-term collaboration and providing the samples for analysis.
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This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic under the project CEITEC 2020 (LQ1601).
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PM performed the experiments, analyzed images, and wrote the manuscript; HC and SK prepared painting probes and revised the manuscript; and JR revised the manuscript. All authors read and approved the final manuscript.
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All procedures performed in this study were in accordance with the ethical standards of the Veterinary Research Institute (Brno, Czech Republic), which complies with the Czech and European Union Legislation for the protection of animals used for scientific purposes. The blood from the living animals was collected by a ZOO veterinarian during other medical procedures. Collaborating Prague ZOO have license issued by the Ministry of the Environment of the Czech Republic (Act No 162/2003 Coll.).
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Communicated by: Maciej Szydlowski
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Musilova, P., Kubickova, S., Cernohorska, H. et al. Comparative chromosome painting in Chacoan peccary, Catagonus wagneri. J Appl Genetics 62, 319–321 (2021). https://doi.org/10.1007/s13353-021-00619-2
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DOI: https://doi.org/10.1007/s13353-021-00619-2