Abstract
The aim of our experiments was to investigate the effect of chromosome 5A on the thiol-dependent redox environment and on the transcription of cold- and vernalization-related genes during the vegetative/generative transition in crowns and leaves of wheat. Chinese Spring, a moderately freezing-tolerant variety, and its more and less tolerant substitution lines — [CS(Ch5A)] and [CS(Tsp5A)], respectively — with different combinations of vernalization alleles were compared. At low temperature, the amount of cystine and glutathione disulphide and the related redox potentials increased in the crowns but not in the leaves. In the crowns of the substitution lines, the concentration and redox state of thiols were different only at the vegetative and double ridge (start of the generative transition) stages. The expression of the vernalization-related VRN1 gene increased significantly during the transition both in the crowns and leaves. The transcription of the freezing tolerance-related CBF14, COR14b and COR39 genes markedly increased in both organs after 2 weeks at 4 °C when the seedlings were still in the vegetative stage. This increment was greater in CS(Ch5A) than in CS(Tsp5A). The Ch5A chromosome in CS genetic background enhanced the expression of CBF regulon even in the generative phase in crown that is the key organ for overwintering and freezing tolerance. At certain developmental stages, both the thiol and the transcript levels differed significantly in the two substitution lines.
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Acknowledgments
This work was supported by the European Union [FP7-KBBE-2011-5, 289842 – ADAPTAWHEAT], by the Hungarian Research Technology and Innovation Fund [EU BONUS 12-1-2012-0024 and TéT_12_CN-1-2012-0002] and by the Hungarian Scientific Research Fund [OTKA K83642, CNK80781]. Ildikó Vashegyi is Bolyai Fellow of the Hungarian Academy of Sciences.
The authors wish to thank A. Horváth and M. Fehér for their help in plant cultivation and treatment and Gabriella Szalai for help in the HPLC measurement of thiols. Thanks are due to R. Boussicut, F. Taulemesse and V. Allard (INRA, UMR 1095 GDEC, France) for providing the VRN1 and VRN3 primer sequences.
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Communicated by: Andrzej Górny
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Boldizsár, Á., Carrera, D.Á., Gulyás, Z. et al. Comparison of redox and gene expression changes during vegetative/generative transition in the crowns and leaves of chromosome 5A substitution lines of wheat under low-temperature condition. J Appl Genetics 57, 1–13 (2016). https://doi.org/10.1007/s13353-015-0297-2
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DOI: https://doi.org/10.1007/s13353-015-0297-2