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Nicaraven-loaded electrospun wound dressings promote diabetic wound healing via proangiogenic and immunomodulatory functions: a preclinical investigation

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Abstract

The current study developed a biopolymer-based wound dressing by electrospinning of Nicaraven-loaded collagen solution. Firstly, collagen was dissolved in acetic acid, and then Nicaraven was added to the polymeric solution at three different concentrations of 2 w/w%, 4 w/w%, and 6 w/w%. The resulting solution was then electrospun. Various experiments were performed to characterize the produced wound dressings. In vitro studies showed that Nicaraven-loaded scaffolds were not toxic against L929 fibroblast cells and protected them against oxidative stress. Wound healing potential of different formulations of Nicaraven-loaded collagen wound dressings was studied in a rat model of the excisional diabetic wound. The study showed that the collagen/4% Nicaraven and collagen/6% Nicaraven wound dressings exhibited a significantly higher percentage of wound closure, the thickness of the epithelium, and collagen deposition compared with collagen/2% Nicaraven, collagen-only, and sterile gauze groups. Gene expression study showed that the developed wound dressings reduced the tissue expression levels of glutathione peroxidase, NFKβ, and matrix metalloproteinase 9 (MMP9) genes. In addition, in the wounds treated with collagen/4% Nicaraven and collagen/6% Nicaraven scaffolds, wound healing was associated with a higher tissue expression level of b-FGF, VEGF, and collagen type I genes. Overall, wound healing activity of collagen/4% Nicaraven and collagen/6% Nicaraven wound dressings was not significantly different. This study implies that collagen wound dressings incorporated with 4% and 6% Nicaraven can be considered a potential candidate to treat diabetic wounds in the clinic.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia

    Marwah Suliman Maashi

  2. Regenerative Medicine Unit, King Fahad Medical Research Centre, Jeddah, 11211, Saudi Arabia

    Marwah Suliman Maashi

  3. Department of Medical Laboratory Science, Fakeeh College for Medical Sciences, Jeddah, Saudi Arabia

    Shatha G. Felemban

  4. Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia

    Hibah Ali Almasmoum

  5. German Cancer Research Center, Toxicology and Chemotherapy Unit Heidelberg, 69120, Heidelberg, Germany

    Mostafa Jarahian

Authors
  1. Marwah Suliman Maashi
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  2. Shatha G. Felemban
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  3. Hibah Ali Almasmoum
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  4. Mostafa Jarahian
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Contributions

Marwah Suliman Maashi and Shatha G. Felemban contributed to the study conception and design. Material preparation, data collection, and analysis were performed by all the authors. The first draft of the manuscript was written by Marwah Suliman Maashi and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Marwah Suliman Maashi.

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The animal studies were performed in accordance with the U.K. Animals (Scientific Procedures) Act, 1986 and associated guidelines, EU Directive 2010/63/EU for animal experiments. All institutional guidelines were followed in animal experiments and caring.

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Suliman Maashi, M., Felemban, S.G., Almasmoum, H.A. et al. Nicaraven-loaded electrospun wound dressings promote diabetic wound healing via proangiogenic and immunomodulatory functions: a preclinical investigation. Drug Deliv. and Transl. Res. 13, 222–236 (2023). https://doi.org/10.1007/s13346-022-01176-9

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