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Neural tissue regeneration by a gabapentin-loaded cellulose acetate/gelatin wet-electrospun scaffold

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Abstract

In the present study, gabapentin (GBP), an anticonvulsant drug used as an analgesic to control the neuropathic pains, was incorporated with cellulose acetate (CA) and gelatin (Gel) in order to develop a potential scaffold for neural tissue engineering applications. The wet-electrospinning method was used to produce the drug-loaded three-dimensional scaffolds from CA/Gel [1:1 (w/w)] solution in the water/ethanol (3:7) (v/v) coagulation baths containing 3%, 6% and 12% (w/v) of GBP. The scaffolds were evaluated regarding their morphology, contact angle, porosity, tensile strength and cellular response. The scaffold obtained from 6% (w/v) GBP bath was chosen as the optimum scaffold for further in vivo study in a sciatic nerve defect model in Wistar rats. The results of sciatic functional index, hot plate latency, weight-loss percentage of the wet gastrocnemius muscle and the histopathological examination using hematoxylin–eosin staining demonstrated that the GBP-containing scaffold significantly enhanced the regeneration of the created injury, which demonstrates its applicability for neural tissue engineering applications.

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Author notes

  1. Saeed Farzamfar and Mahdi Naseri-Nosar are co-first authors and contributed equally to this work.

Authors and Affiliations

  1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Saeed Farzamfar, Farshid Esmaeilpour & Hadi Samadian

  2. Tissue Engineering and Stem Cell Research Center, Shahroud University of Medical Sciences, Shahroud, 3613773955, Iran

    Mahdi Naseri-Nosar & Majid Salehi

  3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Ahmad Vaez & Arash Goodarzi

  4. Department of Mechanical and Aerospace Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Arian Ehterami

  5. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Hamed Sahrapeyma

  6. Hematology, Oncology and Stem Cell Transplantation Children Medical Center, Tehran University of Medical Sciences, Tehran, Iran

    Amir-Ali Hamidieh

  7. Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Sadegh Ghorbani & Arian Azimi

Authors
  1. Saeed Farzamfar
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  2. Mahdi Naseri-Nosar
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Correspondence to Majid Salehi.

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Farzamfar, S., Naseri-Nosar, M., Vaez, A. et al. Neural tissue regeneration by a gabapentin-loaded cellulose acetate/gelatin wet-electrospun scaffold. Cellulose 25, 1229–1238 (2018). https://doi.org/10.1007/s10570-017-1632-z

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  • DOI: https://doi.org/10.1007/s10570-017-1632-z

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