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Diagnosis of a new variant of soybean yellow mottle mosaic virus with extended host-range in India

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Abstract

Soybean yellow mottle mosaic virus (SYMMV, genus Carmovirus) was previously known to occur in South Korea and USA causing bright yellow mosaic in soybean. In this study, SYMMV (Car-Mb14 isolate) was isolated from mungbean (Vigna radiata) exhibiting mild mottling and puckering symptoms in the experimental field at Indian Agricultural Research Institute, New Delhi during 2012. The virus isolate, Car-Mb14 induced veinal mottling, mild mottling, chlorotic blotching, local and systemic necrosis in soybean, mungbean, blackgram, French bean and guar bean, respectively. The symptomatology of the present isolate of SYMMV was different from the previously reported South Korean isolate, as the later did not induce symptoms in any of the above legumes other than soybean. The present isolate was phylogenetically distinct and shared 90–93 % sequence identity in coat protein (CP) of 52 SYMMV isolates reported from Korea and USA. In order to know the serological relationships, the CP gene of the present isolate was over expressed as a 39 kDa protein in E. coli and an antiserum of 1:16,000 titer against the recombinant CP was produced. Serological cross reactivity analysis revealed that SYMMV was serologically related to blackgram mottle virus but not to cowpea mottle virus, the other legume infecting carmoviruses. The antiserum was used to detect prevalence of SYMMV in legume crops by ELISA. Out of 145 field samples of legumes (mungbean, blackgram, French bean and soybean) collected from different places in India, SYMMV was detected only in 16 samples of mungbean and one sample of blackgram. The natural infection of SYMMV in mungbean and blackgram was further confirmed based on CP gene sequence. This study provides evidence of occurrence of a new variant of SYMMV with distinct symptom phenotype and extended host-range in India.

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Acknowledgments

The INSPIRE fellowship to the first author provided by Department of Science and Technology (DST), New Delhi and the financial support by National Agricultural Science Fund, ICAR is thankfully acknowledged.

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Authors and Affiliations

  1. Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute (IARI), New Delhi, 110012, India

    Nagamani Sandra, Alok Kumar, Reetika Kapoor, Rakesh Kumar Jain & Bikash Mandal

  2. Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu (SKUAST-J), Jammu and Kashmir, 180009, India

    Prachi Sharma

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  1. Nagamani Sandra
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  2. Alok Kumar
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  3. Prachi Sharma
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  4. Reetika Kapoor
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  5. Rakesh Kumar Jain
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  6. Bikash Mandal
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Correspondence to Bikash Mandal.

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Sandra, N., Kumar, A., Sharma, P. et al. Diagnosis of a new variant of soybean yellow mottle mosaic virus with extended host-range in India. VirusDis. 26, 304–314 (2015). https://doi.org/10.1007/s13337-015-0288-2

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  • DOI: https://doi.org/10.1007/s13337-015-0288-2

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