Skip to main content
SpringerLink
Log in

Survey, symptomatology, transmission, host range and characterization of begomovirus associated with yellow mosaic disease of ridge gourd in southern India

  • Original Article
  • Published:
VirusDisease Aims and scope Submit manuscript

Abstract

Ridge gourd is an important vegetable crop and is affected by several biotic and abiotic factors. Among the different biotic factors, ridge gourd yellow mosaic disease (RgYMD) is new emerging threat for the production of ridge gourd. The incidence of the RgYMD varied from 30 to 100% in southern India with highest disease incidence of 100% observed in Belagavi district of Karnataka state. The infected plants showed chlorosis, mosaic, cupping of leaves, blistering, reduction in leaf size and stunted growth. The varieties/hybrids grown in the farmer’s fields were found to be susceptible to the disease. Begomovirus was detected in 61 out 64 samples collected from different areas of southern India. Further, all the samples failed to give amplification for beta and alpha satellites. The transmission studies revealed that single whitefly (Bemisia tabaci) is enough to transmit the virus, however, 100% transmission was observed with 10 whiteflies. The minimum acquisition access period and inoculation access period for transmission of virus by whitefly was 15 min. Among the 56 host plants belonging to diversified families tested for host range, sponge gourd, ash gourd, bottle gourd, pumpkin, cucumber, summer squash, cluster bean, tobacco and datura were shown to be susceptible. Seventy six varieties/hybrids evaluated for identifying the resistance source for RgYMD, all were found highly susceptible. Sequence analysis of DNA-A revealed that the causal virus shared highest nucleotide sequence identity (92.3%) with Tomato leaf curl New Delhi virus (ToLCNDV) infecting sponge gourd from northern India. Sequence and phylogenetic analysis of both DNA-A and DNA-B components showed that the begomovirus associated with RgYMD is found to be strain of ToLCNDV. This is first report of ToLCNDV association with RgYMD from southern India.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Anirban R, Spoorthi P, Panwar G, Bag MK, Prasad TV, Kumar G, Gangopadhyay KK, Dutta M. Molecular evidence for occurrence of tomato leaf curl New Delhi virus in ash gourd (Benincasa hispida) germplasm showing a severe yellow stunt disease in India. Indian J Virol. 2013;24(1):56–63.

    Google Scholar 

  2. Bandaranayake WMEK, Wickramarachchi WART, Wickramasinghe HAM, Rajapakshe RGAS, Dissanayake DMKK. Molecular detection and characterization of begomoviruses associated with cucurbitaceae vegetables in Sri Lanka. J Natl Sci Found Sri Lanka. 2014;42:265–71.

    Google Scholar 

  3. Berrie LC, Rybicki EP, Rey MEC. Complete nucleotide sequence and host range of South African cassava mosaic virus: further evidence for recombination amongst begomoviruses. J Gen Virol. 2001;82:53–8.

    Article  CAS  PubMed  Google Scholar 

  4. Briddon RW, Mansoor S, Bedford ID, Pinner MS, Saunders K, Stanley J, Zafar Y, et al. Identification of DNA components required for induction of cotton leaf curl disease. Virology. 2001;285:234–43.

    Article  CAS  PubMed  Google Scholar 

  5. Briddon RW, Bull SE, Mansoor S, Amin I, Markham PG. Universal primers for the PCR mediated amplification of DNA-β: a molecule associated with monopartite begomoviruses. Mol Biotechnol. 2002;20:315–8.

    Article  CAS  PubMed  Google Scholar 

  6. Briddon RW, Bull SE, Amin I, Idris AM, Mansoor S, Bedford ID, Dhawan P, et al. Diversity of DNA β, a satellite molecule associated with some monopartite begomoviruses. Virology. 2003;312(1):106–21.

    Article  CAS  PubMed  Google Scholar 

  7. Briddon RW, Bull SE, Amin I, Mansoor S, Bedford ID, Rishi N, Siwatch SS, et al. Diversity of DNA 1; a satellite-like molecule associated with monopartite begomovirus-DNA β complexes. Virology. 2004;324:462–74.

    Article  CAS  PubMed  Google Scholar 

  8. Brown JK, Zerbini FM, Castillo JN, Moriones E, Sobrinho RR, Silva J, Olive EF, et al. Revision of begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol. 2015;60:1593–619.

    Article  Google Scholar 

  9. Bull SE, Briddon RW, Markham PG. Universal primers for the PCR-mediated amplification of DNA 1: a satellite-like molecule associated with begomovirus-DNA β complexes. Mol Biotechnol. 2003;23:83–6.

    Article  CAS  PubMed  Google Scholar 

  10. Chakravarthy ML. Monograph of Indian cucurbitaceous (Taxonomy and distribution). Rec Bot Surv India. 1959;17:6–7.

    Google Scholar 

  11. Chakravarty HH. Cucurbits in India and their role in development of vegetable crops. In: Bates DM, Robinson RW, Jeffrey C, editors. Biology and utilization of the Cucurbitaceae. Ithaca, New York: Cornell University Press; 1990. p. 325–48.

    Google Scholar 

  12. Cui XF, Tao XR, Xie Y, Fauquet CM, Zhou XPA. Zhou XPADNA-β associated with Tomato yellow leaf curl China virus is required for symptom induction. J Virol. 2004;78:13966–74.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Gopalakrishnan TR. Vegetable crops. In: Peter KV, editor. Horticulture science series, vol. 4. New Delhi: New India Publishing Agency; 2007. p. 343.

    Google Scholar 

  14. Jayashree K, Pun KB, Doraiswamy S. Virus-vector relationship of yellow vein mosaic virus and whitefly (Bemisia tabaci) in pumpkin. Indian Phytopathol. 1999;52:10–3.

    Google Scholar 

  15. Jeske H, Lutgemeier M, Preib W. DNA forms indicate rolling circle and recombination-dependent replication of Abutilon mosaic virus. EMBO J. 2001;20:6158–67.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Jose J, Usha R. Bhendi yellow vein mosaic disease in India is caused by association of DNA β satellite with a begomovirus. Virology. 2003;305:310–7.

    Article  CAS  PubMed  Google Scholar 

  17. Lazarowitz SG, Lazdins IB. Infectivity and complete nucleotide sequence of the cloned genomic components of a bipartite Squash leaf curl virus with a broad host range phenotype. Virology. 1991;180:58–69.

    Article  CAS  PubMed  Google Scholar 

  18. Li ZH, Xie Y, Zhou XP. Tobacco curly shoot virus DNA-β is not necessary for infection but intensifies symptoms in a host-dependent manner. Phytopathology. 2005;95:902–8.

    Article  CAS  PubMed  Google Scholar 

  19. Lodhi MA, Ye GN, Weeden NF, Reisch B. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol Biol Rep. 1994;12:6–13.

    Article  CAS  Google Scholar 

  20. Mandal B, Muniyappa V. Transmission of Croton yellow vein mosaic virus by Bemisia tabaci: virus-vector relationships. Fitopatol Bras. 1991;16:260–2.

    Google Scholar 

  21. Mansoor S, Briddon RW, Zafar Y, Stanley J. Geminivirus disease complexes: an emerging threat. Trends Plant Sci. 2003;8:128–34.

    Article  CAS  PubMed  Google Scholar 

  22. Martin DP, Murrell B, Golden M, Khoosal A,Muhire B. RDP4:Detection and analysis of recombination patterns in virus genomes. Virus Evol. 2015;1;(1),vev003 doi: 10.1093/ve/vev003.

  23. Maruthi MN, Colvin J, Seal SE, Gibson G, Cooper J. Coadaptation between cassava mosaic geminiviruses and their local vector populations. Virus Res. 2002;86:71–85.

    Article  CAS  PubMed  Google Scholar 

  24. Maruthi MN, Rekha AR, Muniyappa V. Pumpkin yellow vein mosaic disease is caused by two distinct begomoviruses: complete viral sequences and comparative transmission by an indigenous Bemisia tabaci and the introduced B-biotype. OEPP/EPPO Bull. 2007;37:412–9.

    Article  Google Scholar 

  25. Mathew AV, Muniyappa V. Transmission of Indian cassava mosaic virus by Bemisia tabaci. Fitopatol Bras. 1991;16:46–9.

    Google Scholar 

  26. Muniyappa V, Reddy HR. Studies on the yellow mosaic disease of horsegram (Dolichos biflorus L)—virus vector relationships. Mysore J Agri Sci. 1976;10:605–10.

    Google Scholar 

  27. Nagarajaiah SB, Prakash J. Chemical composition and bioactive potential of dehydrated peels of Benincasa hispida, Luffa acutangula and Sechium edule. J Herbs Spices Med Plants. 2015;21:193–202.

    Article  Google Scholar 

  28. Nawaz-Ul-Rehman MS, Nahid NS, Mansoor S, Briddo RW, Fauquet CM. Posttranscriptional gene silencing suppressor activity of the alpha-Rep of non-pathogenic alphasatellites associated with begomoviruses. Virology. 2010;405:300–8.

    Article  CAS  PubMed  Google Scholar 

  29. Padidam M, Sawyer S, Fauquet CM. Possible emergence of new geminiviruses by frequent recombination. Virology. 1999;265:218–25.

    Article  CAS  PubMed  Google Scholar 

  30. Phaneendra C, Rao KRSS, Jain RK, Mandal B. Tomato leaf curl New Delhi virus is associated with pumpkin leaf curl: a new disease in northern India. Indian J Virol. 2012;23:42–5.

    Article  PubMed  Google Scholar 

  31. Reddy KS, Yaraguntaiah RC. Virus vector relationship in leaf curl disease of tomato. Indian Phytopathol. 1981;34:310–3.

    Google Scholar 

  32. Rojas MR, Hagen C, Lucas WJ, Gibertson RL. Exploiting chinks in the plant’s armor: evolution and emergence of geminiviruses. Ann Rev Phytopathol. 2005;43:361–94.

    Article  CAS  Google Scholar 

  33. Rybicki EP. A phylogenetic and evolutionary justification for 3 genera of Geminiviridae. Arch Virol. 1994;139:49–77.

    Article  CAS  PubMed  Google Scholar 

  34. Sanz AI, Fraile A, Garcia-Arenal F, Zhou X, Robinson DJ, Khalid S, Harrison BD. Multiple infection, recombination and genome relationships among begomovirus isolates found in cotton and other plants in Pakistan. J Gen Virol. 2000;81:1839–49.

    Article  CAS  PubMed  Google Scholar 

  35. Saunders K, Bedford ID, Briddon RW, Markham PG, Wong SM, Stanley J. A unique virus complex causes Ageratum yellow vein disease. Proceedings of the National Academy of Sciences. USA. 2000:97:6890–6895.

  36. Schnippenkoetter WH, Martin DP, Hughes F, Fyvie M, Willmen JA, James D, et al. The biological and genomic characterization of three mastreviruses. Arch Virol. 2001;146:1075–88.

    Article  CAS  PubMed  Google Scholar 

  37. Seal SE, Van Den Bosch F, Jeger MJ. Factors influencing begomovirus evolution and their increasing global significance: implications for sustainable control. Crit Rev Pl Sci. 2006;25:23–46.

    Article  Google Scholar 

  38. Seal SE, Jeger MJ, Van Den Bosch F. Begomovirus evolution and disease management. Adv Virus Res. 2006;67:297–316.

    Article  CAS  PubMed  Google Scholar 

  39. Sohrab SS, Yasir M, El-Kafrawy SA, Mousa MAA, Bakhashwain AA. First report of Begomovirus causing yellow mosaic disease of ridge gourd in Saudi Arabia. In: 6th Euro Virology Congress and Expo. 2016, held during 10-12 March, 2016 at Madrid, Spain.

  40. Sohrab SS, Mandal B, Ali A, Varma A. Chlorotic curly stunt: a severe Begomovirus disease of bottle gourd in Northern India. Indian J Virol. 2010;21:56–63.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Stecher SG, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870–4.

    Article  PubMed  Google Scholar 

  42. Strange RN, Scott PR. Plant disease: a threat to global food security. Ann Rev Phytopathol. 2005;43:83–116.

    Article  CAS  Google Scholar 

  43. Tiwari AK, Snehi SK, Singh R, Raj SK, Rao GP, Sharma PK. Molecular identification and genetic diversity among six begomovirus isolates affecting cultivation of cucurbitaceous crops in Uttar Pradesh. India. Arch Phytopathol Plant Prot. 2012;45:62–72.

    Article  CAS  Google Scholar 

  44. Varma A, Malathi VG. Emerging geminivirus problems: a serious threat to crop production. Ann Appl Biol. 2003;142:145–64.

    Article  CAS  Google Scholar 

  45. Venkataravanappa V, Lakshminarayanareddy CN, Salil J, Krishnareddy M. Molecular characterization of distinct bipartite begomovirus infecting bhendi (Abelomoschus esculentus L.) in India. Virus Genes. 2012;44:522–35.

    Article  CAS  PubMed  Google Scholar 

  46. Zhou X, Liu Y, Calvert L, Munoz C, Otim-Nape GW, Robinson DJ, Harrison BD. Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by inter specific recombination. J Gen Virol. 1997;78:2101–11.

    Article  CAS  PubMed  Google Scholar 

  47. Zhou X, Xie Y, Tao X, Zhang Z, Li Z, Fauquet CM. Characterization of DNA-beta associated with begomoviruses in China and evidence for coevolution with their cognate viral DNA-A. J Gen Virol. 2003;84:237–47.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgement

This work supported by Science and Engineering Research Board (SERB), New Delhi, India. We are thankful to Dr. M. S. Kulkarni, Dean, KRC College of Horticulture, Arabhavi, India and The Director, Indian Vegetable Research Institute (IVRI), Varanasi, India for providing laboratory facilities and germplasms respectively.

Author information

Author notes

  1. K. S. Shankarappa

    Present address: Department of Plant Pathology, College of Horticulture, University of Horticultural Sciences, Bagalkot, Bengaluru, Karnataka, 560 065, India

Authors and Affiliations

  1. Department of Plant Pathology, K. R. C. College of Horticulture, Arabhavi, Karnataka, 591 218, India

    Chandrakant V. Patil, S. V. Ramdas, U. Premchand & K. S. Shankarappa

Authors
  1. Chandrakant V. Patil
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. V. Ramdas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. U. Premchand
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. S. Shankarappa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. S. Shankarappa.

Additional information

All authors have contributed equally to this work.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 407 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Patil, C.V., Ramdas, S.V., Premchand, U. et al. Survey, symptomatology, transmission, host range and characterization of begomovirus associated with yellow mosaic disease of ridge gourd in southern India. VirusDis. 28, 146–155 (2017). https://doi.org/10.1007/s13337-017-0376-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13337-017-0376-6

Keywords

Search

Navigation